Recombinant Human Anti-H2N2 HA Antibody (2G1) (CAT#: PABZ-186)

Recombinant Human Antibody (2G1) is capable of binding to H2N2 HA, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-H2N2 HA mAb and CH1-3 region of human IgG and a light chain (LC) encoding VL from anti-H2N2 HA proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. MAbs 8F8, 8M2, and 2G1, that were generated with hybridoma technology from the peripheral blood of middle-aged donors. These three antibodies showed a similar spectrum.

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Figure 1 Microneutralization data of H2/H3-specific human MAbs

Figure 1 Microneutralization data of H2/H3-specific human MAbs

Krause, J. C., Tsibane, T., Tumpey, T. M., Huffman, C. J., Albrecht, R., Blum, D., ... & Basler, C. F. (2012). Human monoclonal antibodies to pandemic 1957 H2N2 and pandemic 1968 H3N2 influenza viruses. Journal of virology, JVI-07158.

Figure 2 Therapeutic efficacy of MAbs against disease caused by the A/Albany/6/1958 H2N2 virus in mice.

Figure 2 Therapeutic efficacy of MAbs against disease caused by the A/Albany/6/1958 H2N2 virus in mice.

Mice were inoculated on day 0 and treated on day 1 with the indicated antibody (8F8, 8M2, 2G1, or a human IgG control) and dose. In each group, six mice were monitored for survival (A) and weight (B). At the 8F8 200-μg dose (P < 0.01), the 8F8 20-μg dose (P < 0.05), the 8M2 200-μg dose (P < 0.01), the 2G1 200-μg dose (P < 0.01), and the 2G1 20-μg dose (P < 0.01), treatment conferred a survival advantage by log rank test.

Krause, J. C., Tsibane, T., Tumpey, T. M., Huffman, C. J., Albrecht, R., Blum, D., ... & Basler, C. F. (2012). Human monoclonal antibodies to pandemic 1957 H2N2 and pandemic 1968 H3N2 influenza viruses. Journal of virology, JVI-07158.


Specifications

  • Immunogen
  • IAV Hemagglutinin
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG
  • Specificity
  • Tested positive against native H2N2 HA
  • Species Reactivity
  • IAV
  • Clone
  • 2G1
  • Applications
  • Neut Assay-Dependent
    FuncS Assay-Dependent

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • The antibody 2G1 has been reported in applications of Neut, FuncS. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.

Target

  • Alternative Names
  • HA; Hemagglutinin

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Mouse Antibody

CAT Product Name Application Type
MRO-274CT Anti-IAV HA Recombinant Antibody (HC31) ELISA, Funcs, IP, Neut
MRO-275CT Anti-IAV HA Recombinant Antibody (HC100) Funcs, IP, Neut
MRO-276CT Anti-IAV HA Recombinant Antibody (12D1) ELISA, WB, Neut, Funcs, IHC, Inhib
MRO-277CT Anti-IAV HA Recombinant Antibody (7A7) ELISA, WB, Neut, Funcs, Inhib, IHC
MRO-278CT Anti-IAV HA Recombinant Antibody (39A4) ELISA, WB, Neut, Funcs, IHC, Inhib

Humanized Antibody

CAT Product Name Application Type
PABX-070-S (P) Recombinant Mouse Anti-HA1 Antibody scFv Fragment (Fab17/9) WB, ELISA, Neut, FuncS scFv

Rabbit Monoclonal Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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