Recombinant Human Anti-H3N2 HA Antibody (F045-092) (CAT#: PABC-233)

Recombinant Human Antibody (F045-092) is capable of binding to H3N2 HA, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-H3N2 HA mAb and CH1-3 region of human IgG and a light chain (LC) encoding VL from anti-H3N2 HA proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. The antibody, F045-092, that possesses broadly neutralizing activity against the entire H3 subtype and accommodates.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Datasheet
  • MSDS
  • COA
WB

Figure 1 Human Anti-H3N2 HA Antibody (F045-092) in WB.

Figure 1 Human Anti-H3N2 HA Antibody (F045-092) in WB.

F045-092 and F026-427 binding to and neutralizing activities against virus strains. Western blot of three H3 strain viruses and one H1 strain virus with Fab-cp3 of four clones under nonreducing conditions.

Ohshima, N., Iba, Y., Kubota-Koketsu, R., Asano, Y., Okuno, Y., & Kurosawa, Y. (2011). Naturally occurring antibodies in humans can neutralize a variety of influenza virus strains, including H3, H1, H2, and H5. Journal of Virology, 85(21), 11048-11057.

ELISA

Figure 2 Human Anti-H3N2 HA Antibody (F045-092) in ELISA.

Figure 2 Human Anti-H3N2 HA Antibody (F045-092) in ELISA.

F045-092 and F026-427 binding to and neutralizing activities against virus strains. Binding activities of IgG from F045-092 (gray) and F026-427 (white) to 12 H3 viruses and 1 H1 virus were measured by ELISA. The OD values at 492 nm are indicated.

Ohshima, N., Iba, Y., Kubota-Koketsu, R., Asano, Y., Okuno, Y., & Kurosawa, Y. (2011). Naturally occurring antibodies in humans can neutralize a variety of influenza virus strains, including H3, H1, H2, and H5. Journal of Virology, 85(21), 11048-11057.

Neut

Figure 3 Human Anti-H3N2 HA Antibody (F045-092) in Neut.

Figure 3 Human Anti-H3N2 HA Antibody (F045-092) in Neut.

F045-092 and F026-427 binding to and neutralizing activities against virus strains. Virus-neutralizing activities of F045-092 (green) and F026-427 (red) against H3N2 (Aichi/68, Kitakyushu/93), H3N8 (Budgerigar/Aichi/77), H2N2 (Okuda/57, Duck/Hong Kong/78), H1N1 (New Caledonia/99, Suita/2009 pdm, Swine/Hokkaido/81), and H5N1 (Duck/Mongolia/2001, Vietnam/2004, Anhui/2005, Indonesia/2005) viruses were measured by focus reduction assay.

Ohshima, N., Iba, Y., Kubota-Koketsu, R., Asano, Y., Okuno, Y., & Kurosawa, Y. (2011). Naturally occurring antibodies in humans can neutralize a variety of influenza virus strains, including H3, H1, H2, and H5. Journal of Virology, 85(21), 11048-11057.

FC

Figure 4 Human Anti-H3N2 HA Antibody (F045-092) in FC.

Figure 4 Human Anti-H3N2 HA Antibody (F045-092) in FC.

FCM analyses of the cells expressing HA and HA1 of Aichi/68 virus. FCM signals for mock transfection (gray-filled area), HA-expressing cells (green), and HA1-expressing cells (pink) are shown. F49 Ab binds to an epitope on HA2 that is commonly present on all H3 subtype viruses. Anti-V5 Ab binds to the V5 tag located at the membrane-proximal end of HA. F003-137 binds to the B2/D site located on the globular head of HA.

Ohshima, N., Iba, Y., Kubota-Koketsu, R., Asano, Y., Okuno, Y., & Kurosawa, Y. (2011). Naturally occurring antibodies in humans can neutralize a variety of influenza virus strains, including H3, H1, H2, and H5. Journal of Virology, 85(21), 11048-11057.

ELISA

Figure 5 Human Anti-H3N2 HA Antibody (F045-092) in ELISA.

Figure 5 Human Anti-H3N2 HA Antibody (F045-092) in ELISA.

Competition for binding to HA in ELISA between C179 and F045-092 or F026-427. Formalin-inactivated New Caledonia/99 virus particles were coated onto an immunoplate. (Left) A total of 50 μl of 0.5 μg/ml of C179 was mixed with 50 μl of 2 μg/ml of Fab-cp3 of F026-427, F045-092, or PBS (No Fab-cp3) and applied to the immunoplate. Bound C179 was detected with anti-mouse IgG-HRP. (Right) A total of 50 μl of 0.5 μg/ml of Fab-cp3 of F026-427, F045-092, or PBS (No Fab-cp3) was mixed with 50 μl of 2 μg/ml of C179 (C179+) or with 50 μl PBS (C179−) and applied to the immunoplate.

Ohshima, N., Iba, Y., Kubota-Koketsu, R., Asano, Y., Okuno, Y., & Kurosawa, Y. (2011). Naturally occurring antibodies in humans can neutralize a variety of influenza virus strains, including H3, H1, H2, and H5. Journal of Virology, 85(21), 11048-11057.

FC

Figure 6 Human Anti-H3N2 HA Antibody (F045-092) in FC.

Figure 6 Human Anti-H3N2 HA Antibody (F045-092) in FC.

Effect of one amino acid substitution at residue 136 of HA on the binding activity of F045-092 and F026-427. Amino acid sequences of HA1 of various strains. The sequence of the Aichi/68 H3N2 virus strain was used as a standard for comparison. The bars indicate the same amino acid as in the standard. Amino acids that differ from those of the standard are indicated by using a different color. Amino acid sequences in sites A, B1, B2, and D are boxed. The amino acids at residue 136 are shaded in yellow.

Ohshima, N., Iba, Y., Kubota-Koketsu, R., Asano, Y., Okuno, Y., & Kurosawa, Y. (2011). Naturally occurring antibodies in humans can neutralize a variety of influenza virus strains, including H3, H1, H2, and H5. Journal of Virology, 85(21), 11048-11057.

ELISA

Figure 4 Human Anti-H3N2 HA Antibody (F045-092) in ELISA.

Figure 4 Human Anti-H3N2 HA Antibody (F045-092) in ELISA.

F045-092 and F026-427 recognize HA1 and show HI activities.Competition for binding to HA in ELISA between C179 and F045-092 or F026-427. Formalin-inactivated New Caledonia/99 virus particles were coated onto an immunoplate. (Left) total of 50 μl of 0.5 μg/ml of C179 was mixed with 50 μl of 2 μg/ml of Fab-cp3 of F026-427, F045-092, or PBS (No Fab-cp3) and applied to the immunoplate.

Ohshima, N., Iba, Y., Kubota-Koketsu, R., Asano, Y., Okuno, Y., & Kurosawa, Y. (2011). Naturally occurring antibodies in humans can neutralize a variety of influenza virus strains, including H3, H1, H2, and H5. Journal of Virology, 85(21), 11048-11057.

FC

Figure 5 Human Anti-H3N2 HA Antibody (F045-092) in FC.

Figure 5 Human Anti-H3N2 HA Antibody (F045-092) in FC.

F045-092 and F026-427 recognize HA1 and show HI activities. FCM analyses of the cells expressing HA and HA1 of Aichi/68 virus. FCM signals for mock transfection (gray-filled area), HA-expressing cells (green), and HA1-expressing cells (pink) are shown. F49 Ab binds to an epitope on HA2 that is commonly present on all H3 subtype viruses.

Ohshima, N., Iba, Y., Kubota-Koketsu, R., Asano, Y., Okuno, Y., & Kurosawa, Y. (2011). Naturally occurring antibodies in humans can neutralize a variety of influenza virus strains, including H3, H1, H2, and H5. Journal of Virology, 85(21), 11048-11057.


Specifications

  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG
  • Specificity
  • Tested positive against native H3N2 HA
  • Species Reactivity
  • H3N2
  • Clone
  • F045-092
  • Applications
  • Can be useful in applications such as: Neutralization

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Target

  • Alternative Names
  • Hemagglutinin; HA; H3N2; Influenza A virus

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone F045-092"

See other products for "HA"

Humanized Antibody

CAT Product Name Application Type
PABX-070-S (P) Recombinant Mouse Anti-HA1 Antibody scFv Fragment (Fab17/9) WB, ELISA, Neut, FuncS scFv

Rabbit Monoclonal Antibody

Submit a review or a question
There are currently no Customer reviews or questions for PABC-233. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

© 2024 Creative Biolabs.
  • 0
  • 0
Cart

    Go to compare