Recombinant Human Anti-H3N2 HA Antibody (F045-092) (CAT#: PABC-233)
Recombinant Human Antibody (F045-092) is capable of binding to H3N2 HA, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-H3N2 HA mAb and CH1-3 region of human IgG and a light chain (LC) encoding VL from anti-H3N2 HA proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. The antibody, F045-092, that possesses broadly neutralizing activity against the entire H3 subtype and accommodates.
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SDS-PAGE
Figure 1 Recombinant Human Anti-H3N2 HA Antibody (F045-092) (PABC-233) in SDS-PAGE
SDS-PAGE analysis of PABC-233 In non-reduced (lane 1) and β-mercaptoethanol-reduced (lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 130-180 kDa.
ELISA
Figure 2 Recombinant Human Anti-H3N2 HA Antibody (F045-092) (PABC-233) in ELISA
ELISA analysis of PABC-233 was performed by coating with Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag).
The secondary antibody: HRP-Anti-Human IgG (H+L)
ELISA
Figure 3 Recombinant Human Anti-H3N2 HA Antibody (F045-092) (PABC-233) in ELISA
ELISA analysis of PABC-233 was performed by coating with Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag).
The secondary antibody: HRP-Anti-Human IgG (H+L)
ELISA
Figure 4 Recombinant Human Anti-H3N2 HA Antibody (F045-092) (PABC-233) in ELISA
ELISA analysis of PABC-233 was performed by coating with Influenza A H2N2 (A/Japan/305/1957) Hemagglutinin / HA Protein (His Tag).
The secondary antibody: HRP-Anti-Human IgG (H+L)
WB
Figure 5 Recombinant Human Anti-H3N2 HA Antibody (F045-092) (PABC-233) in WB
Western blot analysis of PABC-233 was performed by loading Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag), Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag), Influenza A H2N2 (A/Japan/305/1957) Hemagglutinin / HA Protein (His Tag).
PABC-233 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Left: Reducing Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag) (0.8 μg)
Middle: Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag) (0.8 μg)
Right: Influenza A H2N2 (A/Japan/305/1957) Hemagglutinin / HA Protein (His Tag) (0.8 μg)
DB
Figure 6 Recombinant Human Anti-H3N2 HA Antibody (F045-092) (PABC-233) in DB
Dot Blot analysis of PABC-233 was performed by coating with Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag), Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag), Influenza A H2N2 (A/Japan/305/1957) Hemagglutinin / HA Protein (His Tag).
PABC-233 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Top: Reducing Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag)
Middle: Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag)
Bottom: Influenza A H2N2 (A/Japan/305/1957) Hemagglutinin / HA Protein (His Tag)
Specifications
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native H3N2 HA
- Species Reactivity
- H3N2
- Clone
- F045-092
- Applications
- Can be useful in applications such as: Neutralization
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
- Alternative Names
- Hemagglutinin; HA; H3N2; Influenza A virus
- UniProt ID
- R9U684
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-203 | Recombinant Human Anti-IAV HA Antibody scFv Fragment (3.1) | WB, ELISA, FuncS | scFv |
| PSBL-204 | Recombinant Human Anti-IAV HA Antibody scFv Fragment (39.29) | Neut, FuncS | scFv |
| PSBL-205 | Recombinant Human Anti-IAV HA Antibody scFv Fragment (1957) | WB, Neut, FuncS | scFv |
| PSBL-206 | Recombinant Human Anti-IAV HA Antibody scFv Fragment (1F1) | WB, ELISA, FuncS | scFv |
| PSBL-207 | Recombinant Human Anti-IAV HA Antibody scFv Fragment (5J8) | WB, ELISA, FuncS | scFv |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABC-233, RRID: AB_3111579)
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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