Mouse Anti-E2F1 Recombinant Antibody (clone 4G8-2C2-C12) (CAT#: VS3-QX336)
This product is a mouse antibody that recognizes E2F1. The antibody can be used in applications: WB, IF, IP.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to centrosome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29735/1143_F6_4_selected.jpg
(Immunofluorescent staining of human cell line RH-30 shows localization to centrosome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29735/1143_F6_4_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & centrosome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/329/951_D1_2_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & centrosome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/329/951_D1_2_selected.jpg
Figure 3 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8003/ihc_selected.jpg
(Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8003/ihc_selected.jpg
Figure 4 IHC staining of human colon
(Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/ihc_selected.jpg
(Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/ihc_selected.jpg
Figure 5 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_B_9_5.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_B_9_5.jpg
Figure 6 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_3.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_3.jpg
Figure 7 Liver
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_4.jpg
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_4.jpg
Figure 8 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_5.jpg
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_5.jpg
Figure 9 Testis
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_4_6.jpg
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_4_6.jpg
Figure 10 Bone marrow
(Hematopoietic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_B_4_4.jpg
(Hematopoietic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_B_4_4.jpg
Figure 11 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_9_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_9_8.jpg
Figure 12 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101412-E2F1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101412-E2F1
Specifications
- Immunogen
- Purified recombinant human E2F-1 protein fragments expressed in E. coli.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2b
- Specificity
- E2F-1
- Species Reactivity
- Human, Rat
- Clone
- 4G8-2C2-C12
- Applications
- WB, IF, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) and 50% glycerol.
- Preservative
- 0.03% Proclin300
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
Bladder Cancer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4001z | Mouse Anti-E2F1 Recombinant Antibody (clone 30B9) | WB, IP, IF, ELISA | Mouse IgG2b, κ |
| MOB-0156CT | Recombinant Mouse anti-Human E2F1 Monoclonal antibody (8G9) | ICC/IF, IP, WB | |
| ZG-0266J | Mouse Anti-E2F1 Recombinant Antibody (ZG-0266J) | WB, ICC | Mouse IgG |
| VS3-FY466 | Recombinant Rabbit Anti-E2F1 Antibody (clone R03-5G3) | WB, IHC-F, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS3-FY2693 | Mouse Anti-E2F1 Recombinant Antibody (VS3-FY2693) | WB, ICC, IP | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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