Anti-Human MIF Recombinant Antibody (TAB-H41)
CAT#: TAB-H41
Recombinant human antibody to Human MIF
Tested Data
Gene Expression
SDS-PAGE
Figure 1 Anti-Human MIF Recombinant Antibody (TAB-H41) in SDS-PAGE
SDS-PAGE analysis of TAB-H41 in β-mercaptoethanol-reduced (Lane 1, 1.5 μg) and non-reduced (Lane 2, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 2 Anti-Human MIF Recombinant Antibody (TAB-H41) in SEC-HPLC
The purity of TAB-H41 was greater than 98% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
DB
Figure 3 Anti-Human MIF Recombinant Antibody (TAB-H41) in DB
Dot Blot analysis of TAB-H41 was performed by coating with human MIF protein (His tag).
TAB-H41-hIgG1 incubation concentration: 2 μg/mL.
HRP conjugated Anti-Human IgG Fc as a secondary antibody (1: 2000)
ELISA
Figure 4 Anti-Human MIF Recombinant Antibody (TAB-H41) in ELISA
ELISA analysis of TAB-H41 was performed by coating with human MIF protein (His tag). Then blocked with BSA and incubated with TAB-H41-hIgG1. The HRP conjugated Anti-human IgG Fc as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 5 Anti-Human MIF Recombinant Antibody (TAB-H41) in WB
WB analysis of TAB-H41 was performed by coating with human MIF protein (His tag).
TAB-H41 incubation concentration: 1 μg/mL.
HRP conjugated Anti-Human IgG Fc as a secondary antibody (1: 2000)
Lane 1: Reduced Antigen (0.1 μg)
Lane 2: Reduced Antigen (0.3 μg)
Lane 3: Reduced Antigen (0.6 μg)
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3868/if_selected.jpg
Normal Tissue
Figure 2 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_8_5.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_5_6.jpg
Normal Tissue
Figure 4 Prostate
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5284/13362_A_1_5.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (HEK93)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000240972-MIF
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Specifications
- Host Species
- human
- Derivation
- human
- Type
- IgG1 - kappa
- Specificity
- MIF (macrophage migration inhibitory factor, GIF, GLIF, glycosylation-inhibiting factor) [Homo sapiens]
- Species Reactivity
- Human
- Applications
- Suitable for use in FuncS, IF, Neut, ELISA, FC, IP, IHC and most other immunological methods.
- Related Disease
- Cancers
Product Property
- Purity
- >95.0% as determined by Analysis by RP-HPLC & analysis by SDS-PAGE.
Target
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Effective MIF Inhibition
The Anti-MIF Recombinant Antibody (TAB-H41) was highly effective in our functional assays. We used it to neutralize Macrophage Migration Inhibitory Factor activity in immune cell cultures. The antibody showed potent inhibitory effects, helping us elucidate the role of MIF in inflammatory responses. The high purity of the product was evident in the consistent results we obtained.
Reliable Immunofluorescence
We employed this antibody for immunofluorescence staining to visualize intracellular MIF distribution. The staining pattern was specific and localized to the cytoplasm as expected. The recombinant nature of the antibody provides assurance of its specificity, which is crucial for differentiating MIF from other cytokines in complex tissue samples.
Q&As
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Is this antibody suitable for functional inhibition of MIF?
A: Yes, this Recombinant Human Anti-MIF Antibody (TAB-H41) is validated for Functional Studies (FuncS) and Neutralization (Neut). It can be used to inhibit the biological activity of Macrophage Migration Inhibitory Factor in your experimental models.
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What other applications can I use this antibody for?
A: Besides functional assays, this antibody is versatile and suitable for Immunofluorescence (IF), ELISA, Flow Cytometry (FC), Immunoprecipitation (IP), and Immunohistochemistry (IHC). It is a robust tool for comprehensive MIF research.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-H41, RRID: AB_3714369)
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Biosimilar Overview
Please refer to Imalumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Imalumab.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| HPAB-1328LY | Human Anti-MIF Recombinant Antibody (HPAB-1328LY) | Inhib, ELISA | Human IgG |
| CAT | Product Name | Application | Type |
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| HPAB-1328LY-F(E) | Human Anti-MIF Recombinant Antibody; Fab Fragment (HPAB-1328LY-F(E)) | Inhib, ELISA | Human Fab |
| CAT | Product Name | Application | Type |
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| HPAB-1329LY-F(E) | Human Anti-MIF Recombinant Antibody; Fab Fragment (HPAB-1329LY-F(E)) | Inhib, ELISA | Human Fab |
| CAT | Product Name | Application | Type |
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| HPAB-1330LY-F(E) | Human Anti-MIF Recombinant Antibody; Fab Fragment (HPAB-1330LY-F(E)) | Inhib, ELISA | Human Fab |
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