Recombinant monoclonal antibody specifically binds to IAV HA, expressed in Chinese Hamster Ovary cells(CHO).
Figure 1 Lung pathologic findings following PR8 virus challenge.
Histopathology in the lung of PR8-infected mice at 6 or 8 d. Inflammation was most severe in infected mice on day 6. After infection with PR8, WT mice treated with Y8 (WT/Y8) or H36 mAb (WT/H36) exhibited few focal areas of epithelial necrosis and peribronchial hemorrhage; however, the WT mice treated with PBS solution or irrelevant IgG, or FcRn-KO mice inoculated with Y8 mAb, showed severe peribronchial inflammation and bronchiolar necrosis with necrotic epithelial cells present in the lumen, and submucosal edema and vascular congestion. A mixed infiltration of inflammatory cells was present throughout at day 6 after infection. All unprotected animals died between days 6 and 7 after infections. Positive control infected mice had similar bronchiolar necrosis as control IgG-inoculated mice. Mock-infected mice had no lesions.
Bai, Y., Ye, L., Tesar, D. B., Song, H., Zhao, D., Björkman, P. J., ... & Zhu, X. (2011). Intracellular neutralization of viral infection in polarized epithelial cells by neonatal Fc receptor (FcRn)-mediated IgG transport. Proceedings of the national academy of sciences, 108(45), 18406-18411.
Figure 2 Characterization of influenza PR8 HA specific monoclonal antibody.
MDCK cells or their transfectants (1 × 10⁶) were infected with influenza virus PR8 (100 pfu/cell) for 1.5 h at 4 °C (A) or switched to 37 °C (B) for 30 min. Cells were fixed with 3.7% paraformaldehyde for 15 min under either nonpermeabilized or permeabilized (0.2% Triton X-100 in PBS solution) conditions as indicated. Cells were incubated with affinity-purified PR8 HA-specific IgG mAb H36-4 (H36) or Y8 (400 μg/mL), followed by Alexa Fluor 488-conjugated IgG (green). Staining was not observed in the presence of isotype matched IgG. Images were originally obtained at a magnification of 40×. (Scale bars: 5 μm.)
Bai, Y., Ye, L., Tesar, D. B., Song, H., Zhao, D., Björkman, P. J., ... & Zhu, X. (2011). Intracellular neutralization of viral infection in polarized epithelial cells by neonatal Fc receptor (FcRn)-mediated IgG transport. Proceedings of the national academy of sciences, 108(45), 18406-18411.
Figure 3 Characterization of influenza PR8 HA specific monoclonal antibody.
HI titer of H36 and Y8 mAb under different pH conditions. H36 or Y8 mAb (400 μg/mL) was incubated with PR8 virus at pH 5.0 or pH 7.4 buffer conditions for 1 h, readjusted to pH 7.4, and followed by HI assay with 0.5% chicken red blood cells for 30 min at room temperature. HI titers were expressed as the reciprocal of the highest dilution of the mAb that completely inhibited hemagglutination (**P < 0.01).
Bai, Y., Ye, L., Tesar, D. B., Song, H., Zhao, D., Björkman, P. J., ... & Zhu, X. (2011). Intracellular neutralization of viral infection in polarized epithelial cells by neonatal Fc receptor (FcRn)-mediated IgG transport. Proceedings of the national academy of sciences, 108(45), 18406-18411.
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CAT | Product Name | Application | Type |
---|---|---|---|
PABL-203 | Recombinant Human Anti-IAV HA Antibody (3.1) | WB, ELISA, FuncS | IgG |
PABL-204 | Recombinant Human Anti-IAV HA Antibody (39.29) | Neut, FuncS | IgG |
PABL-205 | Recombinant Human Anti-IAV HA Antibody (1957) | WB, Neut, FuncS | IgG |
PABL-206 | Recombinant Human Anti-IAV HA Antibody (1F1) | WB, ELISA, FuncS | IgG |
PABL-207 | Recombinant Human Anti-IAV HA Antibody (5J8) | FuncS | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-203 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (3.1) | WB, ELISA, FuncS | Fab |
PFBL-204 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (39.29) | Neut, FuncS | Fab |
PFBL-205 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (1957) | WB, Neut, FuncS | Fab |
PFBL-206 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (1F1) | WB, ELISA, FuncS | Fab |
PFBL-207 | Recombinant Human Anti-IAV HA Antibody Fab Fragment (5J8) | WB, ELISA, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBW-051 | Recombinant Mouse Anti-H3N2 NA Antibody scFv Fragment (Mem5) | WB, ELISA | scFv |
HPAB-N0122-YC-S(P) | Recombinant Mouse Anti-HA Antibody scFv Fragment (AF4H1K1) | IF, Neut, FuncS | Mouse scFv |
HPAB-N0123-YC-S(P) | Recombinant Humanized Anti-HA Antibody scFv Fragment (809) | Neut, ELISA | Humanized scFv |
HPAB-N0124-YC-S(P) | Recombinant Human Anti-HA Antibody scFv Fragment (mAb6137) | ELISA, Inhib, FuncS | Human scFv |
HPAB-N0125-YC-S(P) | Recombinant Human Anti-HA Antibody scFv Fragment (S9-1-10/5-1) | ELISA, Neut | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-268CT | Anti-IAV HA Recombinant Antibody (D6-1) | WB, FC, IP | Human antibody |
MRO-269CT | Anti-IAV HA Recombinant Antibody (IgA 59) | WB, Neut | Human antibody |
MRO-270CT | Anti-IAV HA Recombinant Antibody (IgA 119) | WB, Neut | Human antibody |
MRO-271CT | Anti-IAV HA Recombinant Antibody (IgA 340) | WB, Neut | Human antibody |
MRO-272CT | Anti-IAV HA Recombinant Antibody (IgG 134) | WB, Neut | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-307CT-S(P) | Anti-IAV HA Recombinant Antibody scFv Fragment (8982) | WB, ELISA | |
MRO-308CT-S(P) | Anti-IAV HA Recombinant Antibody scFv Fragment (8153) | WB, ELISA | |
MRO-309CT-S(P) | Anti-IAV HA Recombinant Antibody scFv Fragment (H36-7) | WB, ELISA | |
MRO-310CT-S(P) | Anti-IAV HA Recombinant Antibody scFv Fragment (H36-5) | WB, ELISA | |
MRO-311CT-S(P) | Anti-IAV HA Recombinant Antibody scFv Fragment (H36-17) | WB, ELISA |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-070-S (P) | Recombinant Mouse Anti-HA1 Antibody scFv Fragment (Fab17/9) | WB, ELISA, Neut, FuncS | scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-4209 | Hi-Affi™ Recombinant Rabbit Anti-HA Monoclonal Antibody (SI287DS) | ELISA, ICC/IF, IF, FC | IgG |
MOR-4210 | Hi-Affi™ Recombinant Rabbit Anti-HA Monoclonal Antibody (SI288DS) | ELISA, ICC/IF, IF, FC | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-1169-FY | Llama Anti-HA Recombinant Single Domain Antibody (HPAB-1169-FY) | Neut | Llama VHH |
HPAB-1170-FY | Llama Anti-HA Recombinant Single Domain Antibody (HPAB-1170-FY) | Neut | Llama VHH |
HPAB-1171-FY | Llama Anti-HA Recombinant Single Domain Antibody (HPAB-1171-FY) | Neut | Llama VHH |
HPAB-1172-FY | Llama Anti-HA Recombinant Single Domain Antibody (HPAB-1172-FY) | Neut | Llama VHH |
HPAB-1173-FY | Llama Anti-HA Recombinant Single Domain Antibody (HPAB-1173-FY) | Neut | Llama VHH |
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