Anti-IAV HA Recombinant Antibody (H36-4) (CAT#: MRO-312CT)

Recombinant monoclonal antibody specifically binds to IAV HA, expressed in Chinese Hamster Ovary cells(CHO).


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IHC

Figure 1 Lung pathologic findings following PR8 virus challenge.

Figure 1 Lung pathologic findings following PR8 virus challenge.

Histopathology in the lung of PR8-infected mice at 6 or 8 d. Inflammation was most severe in infected mice on day 6. After infection with PR8, WT mice treated with Y8 (WT/Y8) or H36 mAb (WT/H36) exhibited few focal areas of epithelial necrosis and peribronchial hemorrhage; however, the WT mice treated with PBS solution or irrelevant IgG, or FcRn-KO mice inoculated with Y8 mAb, showed severe peribronchial inflammation and bronchiolar necrosis with necrotic epithelial cells present in the lumen, and submucosal edema and vascular congestion. A mixed infiltration of inflammatory cells was present throughout at day 6 after infection. All unprotected animals died between days 6 and 7 after infections. Positive control infected mice had similar bronchiolar necrosis as control IgG-inoculated mice. Mock-infected mice had no lesions.

Bai, Y., Ye, L., Tesar, D. B., Song, H., Zhao, D., Björkman, P. J., ... & Zhu, X. (2011). Intracellular neutralization of viral infection in polarized epithelial cells by neonatal Fc receptor (FcRn)-mediated IgG transport. Proceedings of the national academy of sciences, 108(45), 18406-18411.

ICC

Figure 2 Characterization of influenza PR8 HA specific monoclonal antibody.

Figure 2 Characterization of influenza PR8 HA specific monoclonal antibody.

MDCK cells or their transfectants (1 × 10⁶) were infected with influenza virus PR8 (100 pfu/cell) for 1.5 h at 4 °C (A) or switched to 37 °C (B) for 30 min. Cells were fixed with 3.7% paraformaldehyde for 15 min under either nonpermeabilized or permeabilized (0.2% Triton X-100 in PBS solution) conditions as indicated. Cells were incubated with affinity-purified PR8 HA-specific IgG mAb H36-4 (H36) or Y8 (400 μg/mL), followed by Alexa Fluor 488-conjugated IgG (green). Staining was not observed in the presence of isotype matched IgG. Images were originally obtained at a magnification of 40×. (Scale bars: 5 μm.)

Bai, Y., Ye, L., Tesar, D. B., Song, H., Zhao, D., Björkman, P. J., ... & Zhu, X. (2011). Intracellular neutralization of viral infection in polarized epithelial cells by neonatal Fc receptor (FcRn)-mediated IgG transport. Proceedings of the national academy of sciences, 108(45), 18406-18411.

Inhib

Figure 3 Characterization of influenza PR8 HA specific monoclonal antibody.

Figure 3 Characterization of influenza PR8 HA specific monoclonal antibody.

HI titer of H36 and Y8 mAb under different pH conditions. H36 or Y8 mAb (400 μg/mL) was incubated with PR8 virus at pH 5.0 or pH 7.4 buffer conditions for 1 h, readjusted to pH 7.4, and followed by HI assay with 0.5% chicken red blood cells for 30 min at room temperature. HI titers were expressed as the reciprocal of the highest dilution of the mAb that completely inhibited hemagglutination (**P < 0.01).

Bai, Y., Ye, L., Tesar, D. B., Song, H., Zhao, D., Björkman, P. J., ... & Zhu, X. (2011). Intracellular neutralization of viral infection in polarized epithelial cells by neonatal Fc receptor (FcRn)-mediated IgG transport. Proceedings of the national academy of sciences, 108(45), 18406-18411.


Specifications

  • Immunogen
  • Influenza virus PR8 Hemagglutinin
  • Host Species
  • Mouse
  • Derivation
  • Mouse hybridoma cell line
  • Specificity
  • IAV
  • Species Reactivity
  • IAV
  • Clone
  • H36-4
  • Applications
  • IHC, ICC, Inhib, WB, ELISA
  • Related Disease
  • Influenza A infection

Applications

  • Application Notes
  • The ImmunoassayV HA antibody has been reported in applications of Immunohistochemistry, Immunocytochemistry, Inhibition, Western Blot, Enzyme-linked Immunosorbent Assay.

Target

  • Alternative Names
  • HA; hemagglutinin; HA1; HA2

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Human Antibody

Humanized Antibody

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Rabbit Monoclonal Antibody

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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