Rabbit Anti-IL21R Recombinant Antibody (clone SI341DS)
CAT#: MOR-4261
Recombinant Rabbit anti-IL21R monoclonal antibody for ELISA, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42296/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42296/94512_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42296/94512_A_8_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42296/94512_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42296/94512_A_6_6.jpg
Normal Tissue
Figure 6 Bone marrow
Hematopoietic cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42296/94512_B_6_4.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42296/94512_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (Daudi, HDLM-2, Karpas-707, RH-30, RPMI-8226, U-266/70)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000103522-IL21R
❮
❯
Specifications
- Immunogen
- Recombinant human IL-21R protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human IL21R
- Species Reactivity
- Human
- Clone
- SI341DS
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- ELISA 0.1-0.2 μg/mL
Target
REVIEWS AND Q&AS
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Sensitive ELISA Detection
We used clone SI341DS for ELISA detection of IL21R. The recommended concentration of 0.1-0.2 μg/mL worked perfectly, providing a strong signal with low background. The recombinant rabbit format offers great consistency.
Reliable Rabbit Monoclonal
This antibody is a reliable reagent for researching the interleukin-21 receptor. Its production via phage display technology seems to ensure high affinity. We appreciate the purity and the detailed Certificate of Analysis provided.
Q&As
-
What is the recommended concentration for ELISA?
A: For ELISA applications, we recommend using this recombinant rabbit anti-IL21R monoclonal antibody at a concentration of 0.1-0.2 μg/mL. However, optimal conditions should be determined by the user for their specific assay setup.
-
What is the source of this antibody?
A: This antibody is a Hi-Affi™ recombinant rabbit monoclonal antibody developed using phage display library technology. It avoids the use of animals for ascites production and ensures high specificity and sensitivity for the target IL21R.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "IL21R"
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| TAB-583LC | Rat Anti-IL21R Recombinant Antibody (TAB-583LC) | ELISA, WB | Rat IgG |
| CAT | Product Name | Application | Type |
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| TAB-584LC | Rat Anti-IL21R Recombinant Antibody (TAB-584LC) | ELISA, WB | Rat IgG |
| CAT | Product Name | Application | Type |
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| TAB-585LC | Human Anti-IL21R Recombinant Antibody (TAB-585LC) | ELISA, FC | Human IgG2, κ |
| CAT | Product Name | Application | Type |
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| TAB-586LC | Human Anti-IL21R Recombinant Antibody (TAB-586LC) | ELISA, FC | Human IgG2, κ |
| CAT | Product Name | Application | Type |
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| TAB-587LC | Human Anti-IL21R Recombinant Antibody (TAB-587LC) | ELISA, FC | Human IgG2, κ |
| CAT | Product Name | Application | Type |
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| TAB-588LC | Anti-human IL21R Recombinant Antibody (MUF) (TAB-588LC) | ELISA, FC, Block | Human antibody |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| TAB-584LC-S(P) | Rat Anti-IL21R Recombinant Antibody; scFv Fragment (TAB-584LC-S(P)) | ELISA, WB | Rat scFv |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| HPAB-0668-CN-S(P) | Human Anti-IL21R Recombinant Antibody (clone 18A5); scFv Fragment | ELISA, FC, Neut | Human scFv |
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| HPAB-0669-CN-F(E) | Human Anti-IL21R Recombinant Antibody (clone ABS); Fab Fragment | ELISA, FC, Neut | Human Fab |
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| CAT | Product Name | Application | Type |
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| HPAB-0247CQ-F(E) | Human Anti-IL21R Recombinant Antibody (clone 19F5); Fab Fragment | ELISA, FC, Inhib | Human Fab |
| CAT | Product Name | Application | Type |
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| HPAB-0247CQ-S(P) | Human Anti-IL21R Recombinant Antibody (clone 19F5); scFv Fragment | ELISA, FC, Inhib | Human scFv |
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| HPAB-AP685-YC-F(E) | Human Anti-IL21R Recombinant Antibody (clone C2); Fab Fragment | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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