Recombinant Mouse Antibody (HYHEL-63) is capable of binding to Lysozyme.
Figure 1 (A) Binding of Fab HyHEL-63 to immobilized wild-type HEL. (B) Scatchard plot of the binding of Fab HyHEL-63 to wild-type HEL derived from the data depicted in panel A after correction for nonspecific binding. (C) Binding of Fab HyHEL-63 to the immobilized HEL K97A mutant. (D) Scatchard plot derived from the data depicted in panel C after correction for nonspecific binding.
(A) Fab HyHEL-63 was injected at 14 different concentrations ranging from 5.3 to 64 nM over a surface to which 550 RU of wild-type HEL had been coupled. Buffer flow rates were 5 µL/min. Equilibrium binding levels were reached within 50 min. After equilibrium had been reached, residual bound protein was eluted using a 1 min pulse of 10 mM HCl. (B) Req is the corrected equilibrium response at a given concentration C. The plot is linear with a correlation coefficient of 0.99. The apparent KA is 3.6 × 108 M⁻¹. The predicted maximum binding capacity (1900 RU) indicates that about 30% of the immobilized wild-type HEL molecules are available for binding. (C) Fab HyHEL-63 was injected at 10 different concentrations ranging from 0.1 to 3.2 µM over a surface to which 420 RU of HEL K97A had been coupled. (D) The plot is linear with a correlation coefficient of 0.99, and the apparent KA is 9.4 × 105 M⁻¹. Approximately 35% of the immobilized HEL K97A is available for binding as calculated from the predicted maximum binding capacity (1500 RU).
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
For Research Use Only. Not For Clinical Use.