Mouse Anti-LYZ Recombinant Antibody (clone HYHEL-63) (CAT#: PABL-268)

Recombinant Mouse Antibody (HYHEL-63) is capable of binding to Lysozyme.

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Figure 1 (A) Binding of Fab HyHEL-63 to immobilized wild-type HEL. (B) Scatchard plot of the binding of Fab HyHEL-63 to wild-type HEL derived from the data depicted in panel A after correction for nonspecific binding. (C) Binding of Fab HyHEL-63 to the immobilized HEL K97A mutant. (D) Scatchard plot derived from the data depicted in panel C after correction for nonspecific binding.

(A) Fab HyHEL-63 was injected at 14 different concentrations ranging from 5.3 to 64 nM over a surface to which 550 RU of wild-type HEL had been coupled. Buffer flow rates were 5 µL/min. Equilibrium binding levels were reached within 50 min. After equilibrium had been reached, residual bound protein was eluted using a 1 min pulse of 10 mM HCl. (B) Req is the corrected equilibrium response at a given concentration C. The plot is linear with a correlation coefficient of 0.99. The apparent KA is 3.6 × 108 M⁻¹. The predicted maximum binding capacity (1900 RU) indicates that about 30% of the immobilized wild-type HEL molecules are available for binding. (C) Fab HyHEL-63 was injected at 10 different concentrations ranging from 0.1 to 3.2 µM over a surface to which 420 RU of HEL K97A had been coupled. (D) The plot is linear with a correlation coefficient of 0.99, and the apparent KA is 9.4 × 105 M⁻¹. Approximately 35% of the immobilized HEL K97A is available for binding as calculated from the predicted maximum binding capacity (1500 RU).

Li, Y., Li, H., Smith-Gill, S. J., & Mariuzza, R. A. (2000). Three-dimensional structures of the free and antigen-bound Fab from monoclonal antilysozyme antibody HyHEL-63.Biochemistry, 39(21), 6296-6309.

Figure 2 Pareto optimal and near-optimal HEL designs independently disrupting each antibody using 1 to 3 mutations.

Choi, Y., Furlon, J. M., Amos, R. B., Griswold, K. E., & Bailey-Kellogg, C. (2018). DisruPPI: structure-based computational redesign algorithm for protein binding disruption.Bioinformatics, 34(13), i245-i253.


Specifications

  • Immunogen
  • Chicken lysozyme (renal amyloidosis)
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Chicken LYZ
  • Species Reactivity
  • Chicken
  • Clone
  • HYHEL-63
  • Applications
  • WB, ELISA, FuncS

Target

  • Alternative Names
  • LYZ; lysozyme (renal amyloidosis); 1; LYZC; lysozyme C; dystrophin; allergen Gal d IV; egg white lysozyme; 4-beta-N-acetylmuramidase C; 1,4-beta-N-acetylmuramidase C

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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Fab Fragment Antibody

CAT Product Name Application Type
PFBL-267 Human Anti-LYZ Recombinant Antibody (clone HYHEL-10); Fab Fragment WB, IHC, FuncS Human Fab
PFBL-268 Mouse Anti-LYZ Recombinant Antibody (clone HYHEL-63); Fab Fragment WB, ELISA, FuncS Mouse Fab
FAMAB-0212CQ-F(E) Recombinant Mouse Anti-LYZ Antibody Fab Fragment (Hyb.C1) ELISA Mouse Fab
FAMAB-0213CQ-F(E) Recombinant Mouse Anti-LYZ Antibody Fab Fragment (Gloop2) ELISA Mouse Fab
FAMAB-0214CQ-F(E) Recombinant Mouse Anti-LYZ Antibody Fab Fragment (Gloop3) ELISA Mouse Fab

Single-domain Antibody

CAT Product Name Application Type
PNBL-036 Recombinant Anti-Chicken LYZ VHH Single Domain Antibody (1D2L19) WB, FuncS Llama VHH
PNBL-051 Recombinant Anti-Chicken LYZ VHH Single Domain Antibody (cAbBCII-10) ELISA Llama VHH
PNBL-052 Recombinant Anti-LYZ (D67H) VHH Single Domain Antibody (cAb-HuL6) WB, ELISA, FuncS Llama VHH
PNBL-053 Recombinant Anti-Chicken LYZ VHH Single Domain Antibody (cAb-Lys2) WB, ELISA, FuncS Llama VHH
PNBL-054 Recombinant Anti-Chicken LYZ VHH Single Domain Antibody (cAb-Lys3) WB, FuncS Llama VHH

Recombinant Antibody

CAT Product Name Application Type
PABZ-015 Mouse Anti-LYZ Recombinant Antibody (clone HyHEL-5) WB, FuncS Mouse IgG
PABZ-022 Mouse Anti-LYZ Recombinant Antibody (clone D44.1) WB, IF, FuncS Mouse IgG
PABW-022 Human Anti-LYZ Recombinant Antibody (clone dAb HEL4) ELISA Human IgG
PABC-023 Recombinant Mouse Anti-Chicken LYZ Antibody (F10.6.6) WB, IF, FuncS IgG
PABZ-023 Mouse Anti-LYZ Recombinant Antibody (clone F9.13.7) WB, IHC, IF, FuncS Mouse IgG

scFv Fragment Antibody

CAT Product Name Application Type
PSBW-128 Recombinant Mouse Anti-Turkey LYZ Antibody scFv Fragment (1F9) ELISA scFv
PSBC-129 Recombinant Mouse Anti-Turkey LYZ Antibody scFv Fragment (HyHEL-10 (SFSF) WB, ELISA scFv
MOB-111YJL-S(P) Human Anti-LYZ Recombinant Antibody (clone HYHEL-10 Y53D); scFv Fragment ELISA, FC, IHC Human scFv
MOB-112YJL-S(P) Human Anti-LYZ Recombinant Antibody (clone HYHEL-10 Y53D,Y58F); scFv Fragment ELISA, FC, IHC Human scFv
PSBS-0138 Human Anti-LYZ Recombinant Antibody (Clone 01JF38); scFv Fragment ELISA, FuncS Human scFv

Humanized Antibody

CAT Product Name Application Type
PABX-073 Recombinant Human Anti-Lysozyme Antibody (HuLys) WB, ELISA, FuncS IgG
PABX-073-F (E) Recombinant Human Anti-Lysozyme Antibody Fab Fragment (HuLys) WB, ELISA, FuncS Fab

Mouse Antibody

CAT Product Name Application Type
PABX-074 Recombinant Mouse Anti-Lysozyme Antibody (HyHEL-5) WB, ELISA, FuncS IgG
PABX-139 Mouse Anti-LYZ Recombinant Antibody (PABX-139) WB, ELISA, FuncS Mouse IgG
PABX-073-S (P) Recombinant Human Anti-Lysozyme Antibody scFv Fragment (HuLys) WB, ELISA, FuncS scFv
PABX-074-S (P) Recombinant Mouse Anti-Lysozyme Antibody scFv Fragment (HyHEL-5) WB, ELISA, FuncS scFv
PABX-139-F (E) Mouse Anti-LYZ Recombinant Antibody; Fab Fragment (PABX-139-F (E)) WB, ELISA, FuncS Mouse Fab

Human Antibody

CAT Product Name Application Type
PABX-138 Recombinant Human Anti-Lysozyme Antibody WB, Neut, FuncS IgG
PABX-138-S (P) Recombinant Human Anti-Lysozyme Antibody scFv Fragment WB, Neut, FuncS scFv
PABX-139-S (P) Mouse Anti-LYZ Recombinant Antibody; scFv Fragment (PABX-139-S (P)) WB, ELISA, FuncS Mouse scFv
PABX-138-F (E) Recombinant Human Anti-Lysozyme Antibody Fab Fragment WB, Neut, FuncS Fab

MHC Tetramer for Other Applications

CAT Product Name Application Type
MHC-LC1532 PE-H2-Ab1/Gallus gallus LYZ (GAMKRHGLDNYRGYSLGN) MHC Tetramer FCM
MHC-LC1134 APC-H2-Aa/Gallus gallus LYZ (AMKRHGLDNYRGYS) MHC Tetramer FCM
MHC-LC1533 APC-H2-Ab1/Gallus gallus LYZ (GAMKRHGLDNYRGYSLGN) MHC Tetramer FCM
MHC-LC1135 BV421-H2-Aa/Gallus gallus LYZ (AMKRHGLDNYRGYS) MHC Tetramer FCM
MHC-LC1534 BV421-H2-Ab1/Gallus gallus LYZ (GAMKRHGLDNYRGYSLGN) MHC Tetramer FCM

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2112 Hi-Affi™ Recombinant Rabbit Anti-LYZ Monoclonal Antibody (DS2112AB) ICC, IHC-P, IP, WB IgG

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For Research Use Only. Not For Clinical Use.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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