Mouse Anti-LYZ Recombinant Antibody (clone HYHEL-63) (CAT#: PABL-268)

Recombinant Mouse Antibody (HYHEL-63) is capable of binding to Lysozyme.

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Figure 1 (A) Binding of Fab HyHEL-63 to immobilized wild-type HEL. (B) Scatchard plot of the binding of Fab HyHEL-63 to wild-type HEL derived from the data depicted in panel A after correction for nonspecific binding. (C) Binding of Fab HyHEL-63 to the immobilized HEL K97A mutant. (D) Scatchard plot derived from the data depicted in panel C after correction for nonspecific binding.

(A) Fab HyHEL-63 was injected at 14 different concentrations ranging from 5.3 to 64 nM over a surface to which 550 RU of wild-type HEL had been coupled. Buffer flow rates were 5 µL/min. Equilibrium binding levels were reached within 50 min. After equilibrium had been reached, residual bound protein was eluted using a 1 min pulse of 10 mM HCl. (B) Req is the corrected equilibrium response at a given concentration C. The plot is linear with a correlation coefficient of 0.99. The apparent KA is 3.6 × 108 M⁻¹. The predicted maximum binding capacity (1900 RU) indicates that about 30% of the immobilized wild-type HEL molecules are available for binding. (C) Fab HyHEL-63 was injected at 10 different concentrations ranging from 0.1 to 3.2 µM over a surface to which 420 RU of HEL K97A had been coupled. (D) The plot is linear with a correlation coefficient of 0.99, and the apparent KA is 9.4 × 105 M⁻¹. Approximately 35% of the immobilized HEL K97A is available for binding as calculated from the predicted maximum binding capacity (1500 RU).

Li, Y., Li, H., Smith-Gill, S. J., & Mariuzza, R. A. (2000). Three-dimensional structures of the free and antigen-bound Fab from monoclonal antilysozyme antibody HyHEL-63.Biochemistry, 39(21), 6296-6309.

Figure 2 Pareto optimal and near-optimal HEL designs independently disrupting each antibody using 1 to 3 mutations.

Choi, Y., Furlon, J. M., Amos, R. B., Griswold, K. E., & Bailey-Kellogg, C. (2018). DisruPPI: structure-based computational redesign algorithm for protein binding disruption.Bioinformatics, 34(13), i245-i253.


Specifications

  • Immunogen
  • Chicken lysozyme (renal amyloidosis)
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Chicken LYZ
  • Species Reactivity
  • Chicken
  • Clone
  • HYHEL-63
  • Applications
  • WB, ELISA, FuncS

Target

  • Alternative Names
  • LYZ; lysozyme (renal amyloidosis); 1; LYZC; lysozyme C; dystrophin; allergen Gal d IV; egg white lysozyme; 4-beta-N-acetylmuramidase C; 1,4-beta-N-acetylmuramidase C

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "LYZ"

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For Research Use Only. Not For Clinical Use.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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