Anti-Mesothelin (MDX-1382)-Duocarmycins ADC (MDX-1204)
CAT#: ADC-L013
This ADC product is composed of an anti-mesothelin antibody (MDX-1382) conjuagated to Duocarmycins (MDX-1382-Duocarmycins). It has demonstrated a response in ovarian and pancreatic cancers treatment by a MOA (Mechanism of Action) of Bind to DNA minor groove and cause DNA damage.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17172/1276_A4_4_selected.jpg
Normal Tissue
Figure 2 Fallopian tube
Ciliated cells (cilia axoneme)
Staining:
High
Intensity: Strong
Ciliated cells (tip of cilia)
Staining:
High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2216/6076_A_7_7.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (CAPAN-2, EFO-21, HeLa, hTCEpi, OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102854-MSLN
❮
❯
Specifications
- Antibody Overview
- Fully human IgG1 monoclonal antibody that binds to mesothelin
- Clone
- MDX-1382
- Antibody Conjugation
- Human
- Linker
- cleavable di-peptide linker
- Linker Class/Description
- Peptide linkers, belonging to Enzymatically cleavable linkers, combine greater systemic stability with rapid enzymatic release of the drug in the target cell. The scission of peptidic bonds relies on lysosomal proteolytic enzymes, which have very low activities in blood due to endogenous inhibitors and the unfavorably high pH value of blood.
- Drug
- Duocarmycins
- Drug Class/Description
- Duocarmycins belong to the minor-groove-binding DNA-alkylating agents (DNA MGBA). The natural duocarmycins are isolated from the culture broth of Streptmyces sp and a series of derivatives has also been synthesized
Target
- Introduction
- This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
- Alternative Names
- MPF; SMRP
- Gene ID
- 10232
- UniProt ID
- Q13421
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for ADC-L013. Click the button above to contact us or submit your feedback about this product.
Powerful DNA Alkylating Agent
The Duocarmycins payload in this ADC is incredibly potent. Our DNA minor groove binding assays confirmed its mechanism of action. It has been a valuable reagent for our studies into high-potency payloads that function independently of the cell cycle phase compared to tubulin inhibitors.
Rapid Enzymatic Release Observed
We tested the cleavable di-peptide linker in this product using lysosomal extract. The release of the toxin was rapid and efficient, consistent with the description of peptide linkers being cleaved by proteolytic enzymes. Targeting of mesothelin-overexpressing cells was also confirmed.
Q&As
-
What is the mechanism of action for the Duocarmycins payload?
A: Duocarmycins belong to the class of minor-groove-binding DNA-alkylating agents. They bind to the minor groove of DNA and cause DNA damage. The natural forms are isolated from Streptomyces, and they are distinct from tubulin inhibitors.
-
How does the cleavable peptide linker function in this ADC?
A: The peptide linker combines systemic stability with rapid enzymatic release. The scission of the peptidic bonds relies on lysosomal proteolytic enzymes. These enzymes have very low activity in the blood due to endogenous inhibitors and pH, ensuring the drug is released only in the target cell.
View the frequently asked questions answered by Creative Biolabs Support.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "MSLN"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1165z | Mouse Anti-MSLN Recombinant Antibody (clone 44C3) | WB, ELISA, IHC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-009 | Recombinant Anti-human MSLN VHH Single Domain Antibody | WB, IHC, FC, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-201 | Anti-Human Mesothelin Recombinant Antibody (TAB-201) | IP, IF, FuncS, FC, Neut, ELISA, IHC | IgG1 - kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G052E | Anti-MSLN immunotoxin RG7787 (Fab)-PE | Cytotoxicity assay, Function study |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G053E | Anti-Msln immunotoxin (IgG)-PE | Cytotoxicity assay, Function study |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L035E | anti-MSLN immunotoxin K1 (IgG)-PE | Cytotoxicity assay, Functional assay |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L063E | anti-MSLN immunotoxin 8B35 (scFv)-PE | Cytotoxicity assay, Functional assay |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-658 | Mouse Anti-MSLN Recombinant Antibody (clone MORAb-009) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-652 | Mouse Anti-MSLN Recombinant Antibody (clone MORAb-009); Fab Fragment | WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-652 | Mouse Anti-MSLN Recombinant Antibody (clone MORAb-009); scFv Fragment | WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0833CL | Human Anti-MSLN Recombinant Antibody (TAB-0833CL) | ELISA, FuncS, Activ, FC | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0834CL | Human Anti-MSLN Recombinant Antibody (TAB-0834CL) | ELISA, FuncS, FC, SPR, Inhib | Human IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0835CL | Human Anti-MSLN Recombinant Antibody (TAB-0835CL) | ELISA, FC, FACS, Inhib | Human IgG1, λ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0833CL-S(P) | Human Anti-MSLN Recombinant Antibody; scFv Fragment (TAB-0833CL-S(P)) | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0834CL-S(P) | Human Anti-MSLN Recombinant Antibody; scFv Fragment (TAB-0834CL-S(P)) | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1467MZ | Recombinant Mouse Anti-Human MSLN Antibody (clone L2) | FC, IHC-Fr, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0376MZ | Chicken Anti-MSLN Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2302 | Hi-Affi™ Recombinant Rabbit Anti-MSLN Monoclonal Antibody (DS2302AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0041-CN | Mouse Anti-MSLN Recombinant Antibody (HPAB-0041-CN) | FC, WB, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0042-CN | Mouse Anti-MSLN Recombinant Antibody (HPAB-0042-CN) | FC, WB, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0041-CN-S(P) | Mouse Anti-MSLN Recombinant Antibody; scFv Fragment (HPAB-0041-CN-S(P)) | FC, WB, ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0042-CN-S(P) | Mouse Anti-MSLN Recombinant Antibody; scFv Fragment (HPAB-0042-CN-S(P)) | FC, WB, ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0043-CN-S(P) | Mouse Anti-MSLN Recombinant Antibody; scFv Fragment (HPAB-0043-CN-S(P)) | FC, WB, ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0045-CN-S(P) | Human Anti-MSLN Recombinant Antibody; scFv Fragment (HPAB-0045-CN-S(P)) | FC, WB, ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0041-CN-F(E) | Mouse Anti-MSLN Recombinant Antibody; Fab Fragment (HPAB-0041-CN-F(E)) | FC, WB, ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0042-CN-F(E) | Mouse Anti-MSLN Recombinant Antibody; Fab Fragment (HPAB-0042-CN-F(E)) | FC, WB, ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0043-CN-F(E) | Mouse Anti-MSLN Recombinant Antibody; Fab Fragment (HPAB-0043-CN-F(E)) | FC, WB, ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0044-CN-F(E) | Human Anti-MSLN Recombinant Antibody; Fab Fragment (HPAB-0044-CN-F(E)) | FC, WB, ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-201 | Afuco™ Anti-MSLN ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-201) | IP, IF, FuncS, FC, Neut, ELISA | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0101 | APC-H-2Db/Human Mesothelin (GQKMNAQAI) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0102 | PE-H-2Db/Human Mesothelin (GQKMNAQAI) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1049 | PE-A*01:01/Human MSLN (TLDTLTAFY) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1050 | APC-A*01:01/Human MSLN (TLDTLTAFY) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1051 | FITC-A*01:01/Human MSLN (TLDTLTAFY) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0391-YC | Recombinant Llama Anti-MSLN Single Domain Antibody (HPAB-M0391-YC) | ELISA, FC, IF | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0392-YC | Recombinant Llama Anti-MSLN Single Domain Antibody (HPAB-M0392-YC) | ELISA, FC, IF | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY69 | Human Anti-MSLN (clone SS) scFv-Fc Chimera | ELISA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC539 | Recombinant Anti-MSLN Vesicular Antibody, EV Displayed (VS-0425-YC539) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4533 | Anti-MSLN Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4534 | Anti-Mouse MSLN Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4535 | Anti-Rat MSLN Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC258 | SmartAb™ Recombinant Anti-MSLN pH-dependent Antibody (VS-0825-YC258) | ELISA, IF, IHC, IP, FC, Neut | Human IgG1 kappa |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, IHC
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: ELISA, WB, BLI, SPR
Application: ELISA, WB, BLI, SPR
Application: WB, ELISA, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
