What is the primary target of the Anti-RNF43 Recombinant Antibody?

The primary target is the RING finger protein 43 (RNF43), a transmembrane E3 ubiquitin ligase involved in regulating Wnt signaling pathways.

In which research applications is this antibody commonly used?

This antibody is commonly used in Western blot (WB), immunoprecipitation (IP), and immunofluorescence (IF) for studying protein expressions and interactions.

What are the specific applications for this recombinant antibody?

Beyond WB, IP, and IF, it can be used in enzyme-linked immunosorbent assays (ELISA) to quantify protein levels and potentially in flow cytometry (FC) for cellular analysis.

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RNF43
Humanized Anti-RNF43 Recombinant Antibody (TAB-328CT)

Humanized Anti-RNF43 Recombinant Antibody (TAB-328CT) (CAT#: TAB-328CT)

This product is a recombinant humanized antibody that recognizes RNF43. The antibody was purified by affinity chromatography. It can be used for WB, ELISA, FC, IHC, IP and other applications.

Tested Data
Gene Expression
Datasheet
MSDS
COA

SDS-PAGE

Figure 1 Anti-Human RNF43 Recombinant Antibody (TAB-328CT) in SDS-PAGE

Figure 1 Anti-Human RNF43 Recombinant Antibody (TAB-328CT) in SDS-PAGE

Lane M: Marker
Lane 1: TAB-328CT in non-reduced condition.
Lane 2: TAB-328CT in reduced condition.

HPLC

Figure 2 Anti-Human RNF43 Recombinant Antibody (TAB-328CT) in HPLC

Figure 2 Anti-Human RNF43 Recombinant Antibody (TAB-328CT) in HPLC

HPLC analysis of TAB-328CT shows the purity is>94%.

Normal Tissue

Figure 1 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8079/23746_A_7_5_selected.jpg

Figure 2 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8079/23746_B_8_5.jpg

Figure 3 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8079/23746_A_9_3.jpg

Figure 4 Kidney
(Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8079/23746_A_7_5.jpg

Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8079/23746_A_6_6.jpg

Figure 6 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8079/23746_A_9_8.jpg

RNA Expression

Figure 7 RNA cell line category: Cell line enhanced (A549, CACO-2, Hep G2, OE19)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108375-RNF43

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Recombinant human RNF43-Fc protein

Host Species
Humanized

Derivation
Mouse hybridoma cell line

Type
Humanized IgG1, κ

Specificity
Human

Species Reactivity
Human

Applications
Used for immunoassay techniques such as: Western Blot; Enzyme-linked Immunosorbent Assay; Flow Cytometry; Immunohistochemistry; Immunoprecipitation

Related Disease
Colorectal cancer,lung cancer.

Product Property

Purity
>95% as determined by SDS-PAGE

Buffer
PBS

Preservative
No preservatives

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
URCC; SSPCS; RNF124

Gene ID
54894

UniProt ID
Q68DV7

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "RNF43"

Select a product category from the dropdown menu below to view related products.

Please select product type

Mouse Antibody Products Humanized Antibody Products MHC Tetramer Products for Cancer

CAT	Product Name	Application	Type
TAB-327CT	Mouse Anti-RNF43 Recombinant Antibody (TAB-327CT)	WB, ELISA, FC, IHC, IP	Mouse IgG
TAB-327CT-S(P)	Mouse Anti-RNF43 Recombinant Antibody; scFv Fragment (TAB-327CT-S(P))	WB, ELISA, FC, IHC, IP	Mouse scFv
TAB-327CT-F(E)	Mouse Anti-RNF43 Recombinant Antibody; Fab Fragment (TAB-327CT-F(E))	WB, ELISA, FC, IHC, IP	Mouse Fab

CAT	Product Name	Application	Type
TAB-328CT-S(P)	Humanized Anti-RNF43 Recombinant Antibody; scFv Fragment (TAB-328CT-S(P))	WB, ELISA, FC, IHC, IP	Humanized scFv
TAB-328CT-F(E)	Humanized Anti-RNF43 Recombinant Antibody; Fab Fragment (TAB-328CT-F(E))	WB, ELISA, FC, IHC, IP	Humanized Fab

CAT	Product Name	Application
MHC-CN1185	PE-A*02:01/Human RNF43 (ALWPWLLMAT) MHC Tetramer	FCM, IHC
MHC-CN1186	APC-A*02:01/Human RNF43 (ALWPWLLMAT) MHC Tetramer	FCM, IHC
MHC-CN1187	FITC-A*02:01/Human RNF43 (ALWPWLLMAT) MHC Tetramer	FCM, IHC
MHC-CN1188	A*02:01/Human RNF43 (ALWPWLLMAT) MHC Tetramer	FCM, IHC
MHC-CN1189	PE-A*24:02/Human RNF43 (NYQPVWLCL) MHC Tetramer	FCM, IHC

Customer Reviews and Q&As

Customer Review Q&As

Reliable in Signal Pathway Analysis

We've used this antibody extensively in analyzing Wnt signaling pathways. Its high specificity and affinity make it reliable for detecting RNF43 interactions and modifications, providing valuable data for our experiments.

Excellent for Western Blotting

We've integrated this antibody into our Western blotting protocols, where it consistently delivers clear, concise results. It's been pivotal for determining RNF43 expression levels under various experimental conditions.

Adaptable for Various Applications

Its adaptability for use in WB, IP, and IF demonstrates its versatility, which is beneficial for labs requiring multi-faceted analysis tools within their experimental setups.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-328CT, RRID: AB_3111913)

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