Is this antibody humanized?

Yes, this is a humanized recombinant antibody targeting SELPLG. It features a human IgG isotype and is expressed in human cells. The antibody is purified by affinity chromatography and is suitable for research into leukocyte trafficking and adhesion.

What applications can I use this antibody for?

This recombinant antibody (TAB-555CL) is reported for use in Enzyme-linked Immunosorbent Assay (ELISA). It recognizes the human SELPLG protein, a glycoprotein that functions as a high-affinity counter-receptor for P-, E-, and L-selectins.

Human Anti-SELPLG Recombinant Antibody (TAB-555CL)

CAT#: TAB-555CL

This product is a recombinant human antibody that recognizes SELPLG. The antibody was purified by affinity chromatography.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line HEL

Immunofluorescent staining of human cell line HEL shows localization to vesicles.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11256/1847_F9_37_cr5abb5d8e30c09_selected.jpg

Normal Tissue Figure 2 High expression in spleen.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2431/6181_B_8_4_val_selected.jpg

Normal Tissue Figure 3 Kidney

Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69062/170043_A_9_5.jpg

Normal Tissue Figure 4 Lymph node

Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69062/170043_A_9_8.jpg

RNA Expression Figure 5 RNA cell line category: Cell line enhanced (HDLM-2, HMC-1, JURKAT, Karpas-707, RPMI-8226, U-266/70, U-266/84)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000110876-SELPLG

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Specifications

Host Species
Human

Derivation
Humanized

Type
Human IgG

Antibody Isotype
IgG

Specificity
Human

Species Reactivity
Human

Applications
ELISA

Related Disease
Autoimmune Diseases; Cancer; Psoriasis

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
SELPLG; selectin P ligand; CLA; CD162; PSGL1; PSGL-1; P-selectin glycoprotein ligand 1; cutaneous lymphocyte-associated associated antigen

Gene ID
6404

UniProt ID
Q14242

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Breast cancer biomarkers at key points during disease progression

Reliable ELISA Detection

We incorporated this recombinant human antibody into our ELISA workflow to detect SELPLG. The affinity was high, and the dose-response curves were reproducible. As a humanized antibody, it served as an excellent tool for our specific assay setup focusing on adhesion molecules.

Pure And Consistent

The SDS-PAGE analysis data provided with the product was accurate; the purity is definitely greater than 95%. We appreciated the stability of the product when stored at -20°C. It has become a standard reagent in our lab for studying P-selectin glycoprotein ligand 1.

Q&As

Is this antibody humanized?

A: Yes, this is a humanized recombinant antibody targeting SELPLG. It features a human IgG isotype and is expressed in human cells. The antibody is purified by affinity chromatography and is suitable for research into leukocyte trafficking and adhesion.
What applications can I use this antibody for?

A: This recombinant antibody (TAB-555CL) is reported for use in Enzyme-linked Immunosorbent Assay (ELISA). It recognizes the human SELPLG protein, a glycoprotein that functions as a high-affinity counter-receptor for P-, E-, and L-selectins.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-555CL.pdf

MSDS

MSDS - TAB-555CL.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

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MOR-3204	Hi-Affi™ Recombinant Rabbit Anti-SELPLG Monoclonal Antibody (DS3204AB)	WB, IP, IHC	IgG

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HPAB-0598-CN-F(E)	Human Anti-SELPLG Recombinant Antibody; Fab Fragment (HPAB-0598-CN-F(E))	ELISA, FC	Humanized Fab

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HPAB-0597-CN	Human Anti-SELPLG Recombinant Antibody (HPAB-0597-CN)	ELISA, FC	Chimeric (mouse/human) IgG1

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HPAB-0597-CN-S(P)	Mouse Anti-SELPLG Recombinant Antibody; scFv Fragment (HPAB-0597-CN-S(P))	ELISA, FC	Mouse scFv

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HPAB-0597-CN-F(E)	Human Anti-SELPLG Recombinant Antibody; Fab Fragment (HPAB-0597-CN-F(E))	ELISA, FC	Human Fab

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HPAB-0101-LS	Mouse Anti-SELPLG Recombinant Antibody (HPAB-0101-LS)	ELISA	Mouse IgG1

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HPAB-0101-LS-S(P)	Mouse Anti-SELPLG Recombinant Antibody; scFv Fragment (HPAB-0101-LS-S(P))	ELISA	Mouse scFv

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HPAB-0102-LS-S(P)	Mouse Anti-SELPLG Recombinant Antibody; scFv Fragment (HPAB-0102-LS-S(P))	ELISA	Mouse scFv

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HPAB-0103-LS-S(P)	Mouse Anti-SELPLG Recombinant Antibody; scFv Fragment (HPAB-0103-LS-S(P))	ELISA	Mouse scFv

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HPAB-0101-LS-F(E)	Mouse Anti-SELPLG Recombinant Antibody; Fab Fragment (HPAB-0101-LS-F(E))	ELISA	Mouse Fab

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HPAB-0102-LS-F(E)	Mouse Anti-SELPLG Recombinant Antibody; Fab Fragment (HPAB-0102-LS-F(E))	ELISA	Mouse Fab

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HPAB-0103-LS-F(E)	Mouse Anti-SELPLG Recombinant Antibody; Fab Fragment (HPAB-0103-LS-F(E))	ELISA	Mouse Fab

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