Anti-Human SPP1 Recombinant Antibody Fab Fragment (mAb 4-4-2)

Name: Anti-Human SPP1 Recombinant Antibody Fab Fragment (mAb 4-4-2)
SKU: TAB-0258CL-F(E)
Rating: 5 (1 reviews)

CAT#: TAB-0258CL-F(E)

The anti-osteopontin antibodies target differen forms of Opn (full length, ThrOpn and MmpOpn), which has different functions in biomineralization, bone remodeling, immune function and heart. The anti-Opn antibody can be used in treatment and/or prevention of chronic inflammatory disease such as type-2 diabetes (T2D) and cardiovascular disease.

Published Data Gene Expression

Figure 1 ELISA of mAbs against either full length Opn (flOpn), thrombin cleaved Opn (ThrOpn), or metalloproteinase cleaved Opn (MmpOpn).

mAb 4-4-2 shows exclusively binding to ThrOpn.

Figure 2 Immunoreactivity of mAbs in Western Blot analysis against either flOpn (lane 1-4), ThrOpn (lane 5-6), or MmpOpn (lane 7-8).

mAb 4-4-2 shows a weak but exclusive binding to ThrOpn.

Figure 3 Assessment of functional activity of mAbs.

(A) Cell adhesion induced by ThrOpn can just be blocked by mAb 21-5-4 mAb 4-4-2 although specific for ThrOpn does not appear to have inhibitory capacities in this setting. (B) Cell adhesion induced by MmpOpn can be blocked by mAbs more reactive towards MmpOpn such as mAb 7-5-4 and 9-3-1 and by the pan-reactive mAb 21-5-4.

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Subcellular Location Figure 1 IF staining of human cell line RPTEC TERT1

Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27540/1789_G12_1_cr59549927dd49e_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line Hep G2

Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/1763_H3_6_selected.jpg

Normal Tissue Figure 3 Kidney

High expression

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/64396_A_7_5_val_selected.jpg

Normal Tissue Figure 4 Colon

Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/64396_A_8_3.jpg

Normal Tissue Figure 5 Gallbladder

Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/64396_A_6_4.jpg

Normal Tissue Figure 6 Lymph node

Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/64396_A_9_8.jpg

RNA Expression Figure 7 RNA cell line category: Cell line enhanced (EFO-21, Hep G2, RPTEC TERT1)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118785-SPP1

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Specifications

Host Species
Mouse

Specificity
Human

Clone
mAb 4-4-2

Applications
ELISA, WB, FuncS

Related Disease
Type-2 diabetes (T2D), cardiovascular disease

Applications

Application Notes
The antibody was validated for ELISA, Western blot and Function assay. For details, refer to Published Data.

Target

Alternative Names
SPP1; secreted phosphoprotein 1; OPN; BNSP; BSPI; ETA-1; osteopontin; uropontin; nephropontin; SPP1/CALPHA1 fusion; urinary stone protein; early T-lymphocyte activation 1; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)

Gene ID
6696

UniProt ID
A0A024RDE6

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-0258CL-F(E).pdf

MSDS

MSDS - TAB-0258CL-F(E).pdf

COA

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