Hi-Affi™ Recombinant Rabbit Anti-SPP1 Monoclonal Antibody (DS3394AB)
CAT#: MOR-3394
Recombinant Rabbit anti-SPP1 monoclonal antibody for FC, ICC/IF, IHC-P, WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RPTEC TERT1
Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27540/1789_G12_1_cr59549927dd49e_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/1763_H3_6_selected.jpg
Normal Tissue
Figure 3 Kidney
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/64396_A_7_5_val_selected.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/64396_A_8_3.jpg
Normal Tissue
Figure 5 Gallbladder
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/64396_A_6_4.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/64396_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (EFO-21, Hep G2, RPTEC TERT1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118785-SPP1
❮
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Specifications
- Immunogen
- Synthetic peptide (Human).
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- IgG
- Species Reactivity
- Human
- Clone
- DS3394AB
- Applications
- Used for immunoassay techniques such as: Flow Cytometry; Immunocytochemistry/Immunofluorescence; Immunohistochemistry (Paraffin); Western Blot
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0843CL | Anti-Human SPP1 Recombinant Antibody (2K1) | sELISA | |
| TAB-0844CL | Anti-Human SPP1 Recombinant Antibody (4C1) | sELISA | |
| TAB-0849CL | Anti-Human SPP1 Recombinant Antibody (2G2) | WB | |
| TAB-0850CL | Anti-Human SPP1 Recombinant Antibody (2H2) | ELISA, FC | |
| TAB-0843CL-S(P) | Anti-Human SPP1 Recombinant Antibody scFv Fragment (2K1) | sELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0846CL | Anti-Human SPP1 Recombinant Antibody (MOR-6990) | ELISA, Neut | Human antibody |
| TAB-0847CL | Anti-Human SPP1 Recombinant Antibody (MOR-6991) | ELISA | Human antibody |
| TAB-0848CL | Anti-Human SPP1 Recombinant Antibody (MOR-6993) | ELISA, FuncS, Neut | Human antibody |
| TAB-0846CL-S(P) | Anti-Human SPP1 Recombinant Antibody scFv Fragment (MOR-6990) | ELISA | Human antibody |
| TAB-0847CL-S(P) | Anti-Human SPP1 Recombinant Antibody scFv Fragment (MOR-6991) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0814MZ | Chicken Anti-Osteopontin Polyclonal IgY | ELISA, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0068-YC | Human Anti-SPP1 Recombinant Antibody (clone h2K1) | FuncS | Humanized IgG |
| HPAB-0069-YC | Human Anti-SPP1 Recombinant Antibody (clone c2K1) | FuncS | Chimeric (mouse/human) IgG, κ |
| HPAB-0070-YC | Human Anti-SPP1 Recombinant Antibody (clone R2K1v1.7) | ELISA | Humanized IgG |
| HPAB-1363-FY | Mouse Anti-SPP1 Recombinant Antibody (clone 1A12) | Inhib, ELISA, WB | Mouse IgG |
| HPAB-1079WJ | Human Anti-SPP1 Recombinant Antibody (clone MOR-6990) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0068-YC-S(P) | Human Anti-SPP1 Recombinant Antibody (clone h2K1); scFv Fragment | FuncS | Humanized scFv |
| HPAB-0069-YC-S(P) | Human Anti-SPP1 Recombinant Antibody (clone c2K1); scFv Fragment | FuncS | Mouse scFv |
| HPAB-0070-YC-S(P) | Human Anti-SPP1 Recombinant Antibody (clone R2K1v1.7); scFv Fragment | ELISA | Humanized scFv |
| HPAB-1079WJ-S(P) | Human Anti-SPP1 Recombinant Antibody (clone MOR-6990); scFv Fragment | ELISA | Human scFv |
| HPAB-0569-YJ-S(P) | Human Anti-SPP1 Recombinant Antibody (clone OPN-3); scFv Fragment | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0068-YC-F(E) | Human Anti-SPP1 Recombinant Antibody (clone h2K1); Fab Fragment | FuncS | Humanized Fab |
| HPAB-0069-YC-F(E) | Human Anti-SPP1 Recombinant Antibody (clone c2K1); Fab Fragment | FuncS | Human Fab |
| HPAB-0070-YC-F(E) | Human Anti-SPP1 Recombinant Antibody (clone R2K1v1.7); Fab Fragment | ELISA | Humanized Fab |
| HPAB-1363-FY-S(P) | Mouse Anti-SPP1 Recombinant Antibody (clone 1A12); Fab Fragment | WB, ELISA | Mouse Fab |
| HPAB-0569-YJ-F(E) | Human Anti-SPP1 Recombinant Antibody (clone OPN-3); Fab Fragment | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY193 | Mouse Anti-SPP1 (clone 23C3) scFv-Fc Chimera | ELISA | Mouse IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6866 | Anti-SPP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6867 | Anti-Mouse SPP1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC228 | Recombinant Anti-SPP1 (Thrombin x MMP cleavage site) Biparatopic Antibody, Tandem scFv (mAb 4-4-2 x mAb 7-5-4) | ELISA, WB | Tandem scFv |
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