Anti-Human SPP1 Recombinant Antibody scFv Fragment (mAb 21-5-4)

Name: Anti-Human SPP1 Recombinant Antibody scFv Fragment (mAb 21-5-4)
SKU: TAB-0261CL-S(P)
Rating: 5 (1 reviews)

CAT#: TAB-0261CL-S(P)

The anti-osteopontin antibodies target differen forms of Opn (full length, ThrOpn and MmpOpn), which has different functions in biomineralization, bone remodeling, immune function and heart. The anti-Opn antibody can be used in treatment and/or prevention of chronic inflammatory disease such as type-2 diabetes (T2D) and cardiovascular disease.

Published Data Gene Expression

Figure 1 ELISA of mAbs against either full length Opn (flOpn), thrombin cleaved Opn (ThrOpn), or metalloproteinase cleaved Opn (MmpOpn).

21-5-4 mAb binds all Opn forms but is more reactive towards cleaved Opn variants.

Figure 2 Immunoreactivity of mAbs in Western Blot analysis against either flOpn (lane 1-4), ThrOpn (lane 5-6), or MmpOpn (lane 7-8).

21-5-4 mAb binds all Opn forms but is more reactive towards cleaved Opn variants.

Figure 3 Assessment of functional activity of mAbs.

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Subcellular Location Figure 1 IF staining of human cell line RPTEC TERT1

Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27540/1789_G12_1_cr59549927dd49e_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line Hep G2

Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/1763_H3_6_selected.jpg

Normal Tissue Figure 3 Kidney

High expression

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/64396_A_7_5_val_selected.jpg

Normal Tissue Figure 4 Colon

Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/64396_A_8_3.jpg

Normal Tissue Figure 5 Gallbladder

Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/64396_A_6_4.jpg

Normal Tissue Figure 6 Lymph node

Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/64396_A_9_8.jpg

RNA Expression Figure 7 RNA cell line category: Cell line enhanced (EFO-21, Hep G2, RPTEC TERT1)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118785-SPP1

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Specifications

Host Species
Mouse

Specificity
Human

Clone
mAb 21-5-4

Applications
ELISA, WB, FuncS

Related Disease
Type-2 diabetes (T2D), cardiovascular disease

Applications

Application Notes
The antibody was validated for ELISA, Western blot and Function assay. For details, refer to Published Data.

Target

Alternative Names
SPP1; secreted phosphoprotein 1; OPN; BNSP; BSPI; ETA-1; osteopontin; uropontin; nephropontin; SPP1/CALPHA1 fusion; urinary stone protein; early T-lymphocyte activation 1; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)

Gene ID
6696

UniProt ID
A0A024RDE6

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-0261CL-S(P).pdf

MSDS

MSDS - TAB-0261CL-S(P).pdf

COA

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