Mouse Anti-TF Recombinant Antibody (clone 5G9) (CAT#: PABW-121)

Figure 1 Inhibition of breast cancer metastasis by coagulation blocking anti-TF Mab-5G9.

Luciferase-tagged MDA-MB-231 cells were mixed with 1 mg of Mab-5G9 and injected into the tail vein of SCID mice. Bioluminescence was measured 4 hours and 1, 2, and 7 days after tumor cell injection.

Versteeg, H. H., Schaffner, F., Kerver, M., Petersen, H. H., Ahamed, J., Felding-Habermann, B., ... & Ruf, W. (2008). Inhibition of tissue factor signaling suppresses tumor growth. Blood, The Journal of the American Society of Hematology, 111(1), 190-199.

Figure 2 Inhibition of proteolytic PAR2 signaling by anti-TF antibodies on MDA-MB-231 human breast cancer cells.

Mab-10H10 and Mab-5G9 inhibit TF-VIIa signaling in MDA-MB-231 cells. Cells were preincubated for 15 minutes with 50 μg/mL Mab-10H10 or Mab-5G9 or 100 μg/mL polyclonalPAR2-cleavage-blocking antibody, followed by addition of 10 nmol/L VIIa, ternary complex (0.5 nmol/L VIIa, 100 nmol/L X,and 200 nmol/L NAPc2) or 10 nmol/L thrombin (IIa). TR3 mRNA induction over control after 90 minutes was determined by quantitative PCR.

Versteeg, H. H., Schaffner, F., Kerver, M., Petersen, H. H., Ahamed, J., Felding-Habermann, B., ... & Ruf, W. (2008). Inhibition of tissue factor signaling suppresses tumor growth. Blood, The Journal of the American Society of Hematology, 111(1), 190-199.

Figure 3 Mab-5G9 stimulates and Mab-10H10 inhibits TF association with β1 integrin in HaCaT cells.

Cells were incubated for 60 minutes with 50 μg/mL Mab-10H10 or Mab-5G9and integrin β1 were immunoprecipitated from lysates.

Versteeg, H. H., Schaffner, F., Kerver, M., Petersen, H. H., Ahamed, J., Felding-Habermann, B., ... & Ruf, W. (2008). Inhibition of tissue factor signaling suppresses tumor growth. Blood, The Journal of the American Society of Hematology, 111(1), 190-199.