Mouse Anti-TF Recombinant Antibody (clone 5G9)

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Q&As

1. Is the anti-TF recombinant antibody clone 5G9 suitable for use in ELISA?

   A: Yes, the anti-TF recombinant antibody clone 5G9 (PABW-121) is suitable for use in ELISA. It has been validated for use in such assays and provides reliable detection of TF.

2. What is the optimal dilution for using the anti-TF recombinant antibody clone 5G9 in Western blotting?

   A: The optimal dilution for using the anti-TF recombinant antibody clone 5G9 (PABW-121) in Western blotting is typically 1:500 to 1:2000. It is advisable to perform a dilution series to determine the best working concentration for your specific experimental conditions.

3. Can the anti-TF recombinant antibody clone 5G9 be used in immunofluorescence assays?

   A: Yes, the anti-TF recombinant antibody clone 5G9 (PABW-121) can be used in immunofluorescence assays. It provides specific binding to TF, allowing for detailed visualization of TF expression in cells.

4. Is the anti-TF recombinant antibody clone 5G9 effective in immunoprecipitation assays?

   A: Yes, the anti-TF recombinant antibody clone 5G9 (PABW-121) is effective in immunoprecipitation assays. It can specifically bind to TF, enabling its precipitation from complex protein mixtures for further analysis.

5. What are the recommended storage conditions for the anti-TF recombinant antibody clone 5G9?

   A: The recommended storage condition for the anti-TF recombinant antibody clone 5G9 (PABW-121) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.

View the frequently asked questions answered by Creative Biolabs Support.

Citations

1. Price, Joshua MJ, et al. "Detection of tissue factor-positive extracellular vesicles using the ExoView R100 system." Research and Practice in Thrombosis and Haemostasis 7.4 (2023): 100177. https://doi.org/10.1016/j.rpth.2023.100177
   This study investigates the potential of using the ExoView R100 system for the detection of tissue factor (TF)-positive extracellular vesicles (EVs) in plasma, particularly from patients with trauma and ovarian cancer. The researchers utilized an antibody-based capture system on ExoView chips to identify and quantify TF+ EVs. The study demonstrated that TF+ EVs can be effectively captured and fluorescently labeled, allowing for their measurement in both cell culture supernatant and plasma samples. The correlation between TF activity and the ExoView measurements suggests that this method could be valuable for clinical applications in identifying individuals at risk of thrombosis.
   Creative Biolabs provided the anti-TF monoclonal antibody 5G9 (Cat# PABW-121), which was used as the capture antibody on the ExoView chips. This antibody was critical for the successful capture and detection of TF+ EVs, enabling the researchers to validate the ExoView system's ability to measure TF+ EVs in various biological samples accurately. The reliable performance of the 5G9 antibody from Creative Biolabs ensured the specificity and sensitivity of the assay, contributing significantly to the study's findings.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# PABW-121, RRID: AB_3111685)

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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