Mouse Anti-TF Recombinant Antibody (clone 5G9) (CAT#: PABW-121)
Recombinant Mouse Antibody (5G9) is capable of binding to TF, expressed in Chinese Hamster Ovary cells (CHO). This antibody binds the carboxyl module of the extracellular domain of TF with a nanomolar binding constant and inhibits the formation of the TF. VIIa. X ternary initiation complex.
Specific Inquiry
BL
Figure 1 Inhibition of breast cancer metastasis by coagulation blocking anti-TF Mab-5G9.
Luciferase-tagged MDA-MB-231 cells were mixed with 1 mg of Mab-5G9 and injected into the tail vein of SCID mice. Bioluminescence was measured 4 hours and 1, 2, and 7 days after tumor cell injection.
Versteeg, H. H., Schaffner, F., Kerver, M., Petersen, H. H., Ahamed, J., Felding-Habermann, B., ... & Ruf, W. (2008). Inhibition of tissue factor signaling suppresses tumor growth. Blood, The Journal of the American Society of Hematology, 111(1), 190-199.
Inhib
Figure 2 Inhibition of proteolytic PAR2 signaling by anti-TF antibodies on MDA-MB-231 human breast cancer cells.
Mab-10H10 and Mab-5G9 inhibit TF-VIIa signaling in MDA-MB-231 cells. Cells were preincubated for 15 minutes with 50 μg/mL Mab-10H10 or Mab-5G9 or 100 μg/mL polyclonalPAR2-cleavage-blocking antibody, followed by addition of 10 nmol/L VIIa, ternary complex (0.5 nmol/L VIIa, 100 nmol/L X,and 200 nmol/L NAPc2) or 10 nmol/L thrombin (IIa). TR3 mRNA induction over control after 90 minutes was determined by quantitative PCR.
Versteeg, H. H., Schaffner, F., Kerver, M., Petersen, H. H., Ahamed, J., Felding-Habermann, B., ... & Ruf, W. (2008). Inhibition of tissue factor signaling suppresses tumor growth. Blood, The Journal of the American Society of Hematology, 111(1), 190-199.
IP
Figure 3 Mab-5G9 stimulates and Mab-10H10 inhibits TF association with β1 integrin in HaCaT cells.
Cells were incubated for 60 minutes with 50 μg/mL Mab-10H10 or Mab-5G9and integrin β1 were immunoprecipitated from lysates.
Versteeg, H. H., Schaffner, F., Kerver, M., Petersen, H. H., Ahamed, J., Felding-Habermann, B., ... & Ruf, W. (2008). Inhibition of tissue factor signaling suppresses tumor growth. Blood, The Journal of the American Society of Hematology, 111(1), 190-199.
Specifications
- Immunogen
- Human coagulation factor III (thromboplastin, tissue factor)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human TF
- Species Reactivity
- Human
- Clone
- 5G9
- Applications
- Block, Inhib, IP
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Immunoprecipitation, Blocking and Inhibition Assay.
Target
- Alternative Names
- F3; coagulation factor III (thromboplastin, tissue factor); TF; TFA; CD142; tissue factor
- Gene ID
- 2152
- UniProt ID
- P13726
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-343 | Mouse Anti-TF Recombinant Antibody (clone 6A6); scFv Fragment | WB, ELISA, IF, FuncS | Mouse scFv |
| HPAB-0767-CN-S(P) | Human Anti-TF Recombinant Antibody; scFv Fragment (HPAB-0767-CN-S(P)) | WB, ELISA, FC, IHC | Human scFv |
| HPAB-0768-CN-S(P) | Human Anti-TF Recombinant Antibody; scFv Fragment (HPAB-0768-CN-S(P)) | WB, ELISA, FC, IHC | Human scFv |
| HPAB-0769-CN-S(P) | Human Anti-TF Recombinant Antibody; scFv Fragment (HPAB-0769-CN-S(P)) | WB, ELISA, FC, IHC | Human scFv |
| HPAB-1276WJ-S(P) | Mouse Anti-TF Recombinant Antibody; scFv Fragment (HPAB-1276WJ-S(P)) | ELISA | Mouse scFv |
Customer Reviews and Q&As
Customer Review
Q&As
-
Ideal for Transferrin Research
The Anti-TF Recombinant Antibody (clone 5G9) has been excellent for our transferrin studies. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time. -
Effective in Immunoassays
We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for transferrin is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab. -
Consistent and High-Quality
This antibody has proven to be consistent and high-quality in our assays. The reproducible results and strong affinity for transferrin have significantly improved our experiments, making it a crucial part of our research toolkit. The consistent performance has greatly enhanced our data quality.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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