Recombinant Mouse Anti-TF(Galβ1-3GalNAcα) Antibody (JAA-F11)
CAT#: PABW-164
Recombinant Mouse Antibody (JAA-F11) is capable of binding to TF antigen(Galβ1-3GalNAcα), expressed in Chinese Hamster Ovary cells (CHO). JAA-F11 preferentially binds to tumor tissue over normal, and in vivo it enhances survival and decreases metastasis in the mouse 4T1 metastatic model, indicating a potential for this mAb to be used, after humanization, in cancer patient therapy.
Gene Expression
Normal Tissue
Figure 1 Liver
Hepatocytes
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5692/159943_A_8_4.jpg
Normal Tissue
Figure 2 Kidney
Cells in tubules
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5692/159943_A_8_5.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enriched (Hep G2)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 3 RNA cell line category: Cell line enriched (Hep G2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000091513-TF
❮
❯
Specifications
- Immunogen
- Human Thomsen-Friedenreich antigen (Galβ1-3GalNAcα)
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against synthetic TF antigen(Galβ1-3GalNAcα)
- Species Reactivity
- Human
- Clone
- JAA-F11
- Applications
- FuncS, IHC, FC, Cyt
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The TF antibody has been reported in applications of Functional Assay, Immunohistochemistry, Flow Cytometry, Cytotoxicity.
For Cyt: The effects of hJAA-F11-DM1 on tumor cell viability were assessed using Cell Titer-Glo reagent. Tumor cells (human breast cancer cell line: MDA-MB-231; human lung cancer cell lines: NCIH520; HTB171; A549) were plated in 96-well flat bottomed plates (1000 cells per well) and allowed to adhere overnight at 37°C in a humidified atmosphere of 5% CO₂. Cell culture medium was then removed and replaced by fresh culture medium containing different concentrations of H2aL2a-DM1, and the plates were incubated for a 5 day time period. After the 5 day time point, 10× Lysis buffer was added to the appropriate positive control wells (for maximum lysis) and the plate was incubated at 37°C for 45 minutes, and then the plate was kept at RT for 30 minutes. Cell Titer-Glo reagent was added to the wells for 10 minute (2 minute on the orbital shaker) at RT and the luminescent signal was measured using a Synergy Neo2 Multi-Mode Reader. IC₅₀ was calculated using ic50.tk.
Target
- Alternative Names
- Galβ1-3GalNAcα; Thomsen-Friedenreich; TF
REVIEWS AND Q&AS
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Reliable in IHC Applications
In our lab, we've employed the JAA-F11 antibody extensively for IHC, where it effectively distinguishes between normal and cancerous tissue. This distinction is critical in cancer research, allowing for more accurate evaluations and insights into tumor biology.
Great for Functional Assays
The antibody's performance in functional assays has been commendable. We've noted significant improvements in our experiments measuring cancer cell viability and response, which aids in evaluating potential therapeutic approaches targeting the TF antigen.
Comprehensive Technical Support
We've always had a positive experience with the technical support provided for the JAA-F11 antibody. Whenever optimization was needed in our assays, their expertise helped us adjust our protocols efficiently and correctly.
Q&As
-
What applications is the JAA-F11 antibody suitable for?
A: The JAA-F11 antibody is compatible with several applications, including Functional Assays, Immunohistochemistry (IHC), Flow Cytometry (FC), and Cytotoxicity tests, allowing researchers to explore various experimental setups.
-
Can this antibody be used in functional studies involving cancer models?
A: Yes, the JAA-F11 antibody can be used in functional assays involving mouse models, as it has shown potential to enhance survival and reduce metastasis, offering insights into cancer therapies.
-
How is the JAA-F11 antibody typically used in IHC?
A: In IHC, this antibody helps visualize the presence and distribution of the TF antigen in tissue samples, aiding in the examination of cancerous tissues versus normal cells.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone JAA-F11"
- CAT
- Product Name
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Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBW-121 | Mouse Anti-TF Recombinant Antibody (clone 5G9); scFv Fragment | WB, IHC, IF, FuncS | Mouse scFv |
| PSBC-122 | Human Anti-TF Recombinant Antibody (clone hATR-5); scFv Fragment | Block | Humanized scFv |
| HPAB-2578LY-S(P) | Human Anti-TF Recombinant Antibody; scFv Fragment (HPAB-2578LY-S(P)) | ELISA | Humanized scfv |
| HPAB-0717YY-S(P) | Human Anti-TF Recombinant Antibody; scFv Fragment (HPAB-0717YY-S(P)) | ELISA, Inhib, WB, FuncS | Human scFv |
| HPAB-0718YY-S(P) | Human Anti-TF Recombinant Antibody; scFv Fragment (HPAB-0718YY-S(P)) | ELISA, Inhib, WB, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0510CL | Mouse Anti-TF Recombinant Antibody (TAB-0510CL) | WB, IF, ELISA | Mouse IgG |
| TAB-0510CL-S(P) | Mouse Anti-TF Recombinant Antibody; scFv Fragment (TAB-0510CL-S(P)) | WB, IF, ELISA | Mouse scFv |
| TAB-598CT | Mouse Anti-TF Recombinant Antibody (TAB-598CT) | ELISA, FC | Mouse IgG1. κ |
| TAB-598CT-S(P) | Mouse Anti-TF Recombinant Antibody; scFv Fragment (TAB-598CT-S(P)) | ELISA, FC | Mouse scFv |
| TAB-598CT-F(E) | Mouse Anti-TF Recombinant Antibody; Fab Fragment (TAB-598CT-F(E)) | ELISA, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-599CT | Anti-Human TF Recombinant Antibody (ATR-5) | ELISA, FC | Humanized antibody |
| TAB-600CT | Anti-Human TF Recombinant Antibody (ATR-2) | ELISA, FC | Humanized antibody |
| TAB-601CT | Anti-Human TF Recombinant Antibody (ATR-8) | ELISA, FC, BL | Humanized antibody |
| TAB-599CT-S(P) | Anti-Human TF Recombinant Antibody scFv Fragment (ATR-5) | ELISA, FC | Humanized antibody |
| TAB-600CT-S(P) | Anti-Human TF Recombinant Antibody scFv Fragment (ATR-2) | ELISA, FC | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-602CT | Anti-Human TF Recombinant Antibody (3e10) | ELISA, BL | Human antibody |
| TAB-603CT | Anti-Human TF Recombinant Antibody (3h2) | ELISA, BL | Human antibody |
| TAB-604CT | Anti-Human TF Recombinant Antibody (2c1) | ELISA, BL | Human antibody |
| TAB-605CT | Anti-Human TF Recombinant Antibody (2c11) | ELISA, BL | Human antibody |
| TAB-606CT | Anti-Human TF Recombinant Antibody (2d3) | ELISA, BL | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-175LC | Recombinant Anti-Human TF Antibody (Non-glycosylated) | ELISA | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0860MZ | Chicken Anti-Transferrin Polyclonal IgY | ELISA | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1996CQ | Mouse Anti-TF Recombinant Antibody (clone HTF-14) | WB, Neut, ELISA, ICC, IF, IHC, IHC-P, RIA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2022CQ | Mouse Anti-TF Recombinant Antibody (clone A68-B/A11) | ELISA, Inhib, IHC-P, IHC-Fr | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3535 | Rabbit Anti-TF Recombinant Antibody (clone DS3535AB) | IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0318CQ | Mouse Anti-TF Recombinant Antibody (clone PTF.02) | ELISA, FC | Mouse IgG1, κ |
| FAMAB-0319CQ | Mouse Anti-TF Recombinant Antibody (clone TW4.20) | ELISA | Mouse IgG1, κ |
| HPAB-0767-CN | Human Anti-TF Recombinant Antibody (HPAB-0767-CN) | WB, ELISA, FC, IHC | Human IgG4 |
| HPAB-0768-CN | Human Anti-TF Recombinant Antibody (HPAB-0768-CN) | WB, ELISA, FC, IHC | Human IgG4 |
| HPAB-0769-CN | Human Anti-TF Recombinant Antibody (HPAB-0769-CN) | WB, ELISA, FC, IHC | Human IgG4 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0207-YC-F(E) | Mouse Anti-TF Recombinant Antibody; Fab Fragment (HPAB-M0207-YC-F(E)) | ELISA, Inhib | Mouse Fab |
| HPAB-M0208-YC-F(E) | Human Anti-TF Recombinant Antibody; Fab Fragment (HPAB-M0208-YC-F(E)) | ELISA, Inhib | Humanized Fab |
| HPAB-M0209-YC-F(E) | Human Anti-TF Recombinant Antibody; Fab Fragment (HPAB-M0209-YC-F(E)) | ELISA, Inhib | Humanized Fab |
| HPAB-M0210-YC-F(E) | Human Anti-TF Recombinant Antibody; Fab Fragment (HPAB-M0210-YC-F(E)) | ELISA, Inhib | Humanized Fab |
| HPAB-M0211-YC-F(E) | Human Anti-TF Recombinant Antibody; Fab Fragment (HPAB-M0211-YC-F(E)) | ELISA, Inhib | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2269 | Anti-TF Immunohistochemistry Kit | IHC | |
| VS-0525-XY7178 | Anti-Human TF Immunohistochemistry Kit | IHC |
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