Recombinant Mouse Anti-TF(Galβ1-3GalNAcα) Antibody (JAA-F11) (CAT#: PABW-164)

• Immunogen
• Human Thomsen-Friedenreich antigen (Galβ1-3GalNAcα)

• Host Species
• Mouse

• Derivation
• Mouse

• Type
• IgG

• Specificity
• Tested positive against synthetic TF antigen(Galβ1-3GalNAcα)

• Species Reactivity
• Human

• Clone
• JAA-F11

• Applications
• FuncS, IHC, FC, Cyt

• Purity
• >95% by SDS-PAGE and HPLC analysis

• Storage
• Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

• Application Notes
• The TF antibody has been reported in applications of Functional Assay, Immunohistochemistry, Flow Cytometry, Cytotoxicity.
  For Cyt: The effects of hJAA-F11-DM1 on tumor cell viability were assessed using Cell Titer-Glo reagent. Tumor cells (human breast cancer cell line: MDA-MB-231; human lung cancer cell lines: NCIH520; HTB171; A549) were plated in 96-well flat bottomed plates (1000 cells per well) and allowed to adhere overnight at 37°C in a humidified atmosphere of 5% CO₂. Cell culture medium was then removed and replaced by fresh culture medium containing different concentrations of H2aL2a-DM1, and the plates were incubated for a 5 day time period. After the 5 day time point, 10× Lysis buffer was added to the appropriate positive control wells (for maximum lysis) and the plate was incubated at 37°C for 45 minutes, and then the plate was kept at RT for 30 minutes. Cell Titer-Glo reagent was added to the wells for 10 minute (2 minute on the orbital shaker) at RT and the luminescent signal was measured using a Synergy Neo2 Multi-Mode Reader. IC₅₀ was calculated using ic50.tk.

• Alternative Names
• Galβ1-3GalNAcα; Thomsen-Friedenreich; TF

• PFBW-164
• Mouse Anti-TF Recombinant Antibody (clone JAA-F11); Fab Fragment

• PSBW-164
• Mouse Anti-TF Recombinant Antibody (clone JAA-F11); scFv Fragment

• PABW-164-mRNA
• Mouse Anti-TF Antibody (clone JAA-F11), mRNA

Select a product category from the dropdown menu below to view related products.