Anti-TFRC immunotoxin HB21 (scFv)-DT (CAT#: AGTO-G067D)
This immunotoxin was achieved by conjugating/fusing the Anti-TFRC scFv to Diphtheria toxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to tumor cells and the toxin enters cells by receptor-mediated endocytosis. Before the N-terminal 193 amino acids corresponding to the catalytic domain of DT enzyme, -II binding and elongation factor into the eukaryotic cell, the cell can catalyze specific elongation factor EF2 amide His699 of diphtheria ADP2 ribosylation occurs, thereby preventing protein synthesis and causes cell death. It was designed for treatment of malignant.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to endosomes & lysosomes.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28598/546_F8_1_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to endosomes & lysosomes.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28598/546_F8_1_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/207_B2_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/207_B2_1_blue_red_green.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/208_B2_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/208_B2_1_blue_red_green.jpg
Figure 4 Cerebral cortex
(Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_B_9_5.jpg
(Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_B_9_5.jpg
Figure 5 Colon
(Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_8_3.jpg
(Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_8_3.jpg
Figure 6 Testis
(Leydig cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Pachytene spermatocytes
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_6_6.jpg
(Leydig cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Pachytene spermatocytes
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_6_6.jpg
Figure 7 Bone marrow
(Hematopoietic cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_B_6_4.jpg
(Hematopoietic cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_B_6_4.jpg
Figure 8 Lymph node
(Germinal center cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_8_8.jpg
(Germinal center cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/153/2800_A_8_8.jpg
Figure 9 RNA cell line category: Cell line enhanced (HDLM-2)
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000072274-TFRC
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000072274-TFRC
Figure 9 RNA cell line category: Cell line enhanced (HDLM-2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000072274-TFRC
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000072274-TFRC
Specifications
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- HB21
- Molecule Class
- scFv-DT
- Toxin Description
- Diphtheria toxin (Diphtheriatoxin, DT) is a cell toxin produced by diphtheria, is a protein toxin most commonly used in the construction of recombinant immunotoxin. Before the N-terminal 193 amino acids corresponding to the catalytic domain of DT enzyme, -II binding and elongation factor into the eukaryotic cell, the cell can catalyze specific elongation factor EF2 amide His699 of diphtheria ADP2 ribosylation occurs, thereby preventing protein synthesis and causes cell death.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- malignant
Target
- Alternative Names
- TFRC; transferrin receptor; T9; TR; TFR; p90; CD71; TFR1; TRFR; transferrin receptor protein 1; transferrin receptor (p90, CD71); Diphtheria toxin; Diphtheria-toxin; DT; DT388; DT389; DT390; DAB447
- Gene ID
- 7037
- UniProt ID
- P02786
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Flow Cytometry (FC) related Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1176z | Mouse Anti-TFRC Recombinant Antibody (MOB-1176z) | ELISA, WB, IP, IF, IHC, FC | Mouse IgG1, κ |
| HPAB-0238-YJ | Rat Anti-TFRC Recombinant Antibody (HPAB-0238-YJ) | IHC, FC, WB, FuncS | Rat IgG2a |
| HPAB-0239-YJ | Mouse Anti-TFRC Recombinant Antibody (HPAB-0239-YJ) | IHC, FC, WB, FuncS | Chimeric (mouse/rat) IgG1, κ |
| HPAB-0240-YJ | Mouse Anti-TFRC Recombinant Antibody (HPAB-0240-YJ) | IHC, FC, WB, FuncS | Chimeric (mouse/rat) IgG1, κ |
| VS-0723-WK21 | Human Anti-TFRC Recombinant Antibody (clone Pabinafusp Alfa) | FC | Human IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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