Recombinant Anti-human VCAM-1 Intrabody [Tat]

Name: Recombinant Anti-human VCAM-1 Intrabody [Tat]
SKU: IAB-B067(T)
Rating: 5 (1 reviews)

CAT#: IAB-B067(T)

Recombinant anti-VCAM-1 intrabody in fusion with HIV Tat works within cells to bind human VCAM-1, expressed in CHO cells.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to cell junctions.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69867/1706_F12_18_cr57d839e75f94b_selected.jpg

Normal Tissue Figure 2 Kidney

Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: Medium Intensity: Strong Quantity: <25%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/154/2835_A_8_5.jpg

Normal Tissue Figure 3 Testis

Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/154/2835_A_6_6.jpg

Normal Tissue Figure 4 Lymph node

Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/154/2835_A_8_8.jpg

Normal Tissue Figure 5 High expression in spleen

Cells in red pulp Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in white pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/154/2835_B_7_4.jpg

RNA Expression Figure 6 RNA cell line category: Group enriched (HHSteC, HSkMC)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162692-VCAM1

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Specifications

Immunogen
human vascular cell adhesion molecule 1

Host Species
mouse

Type
scFv-Tat

Cell Penetrating Peptide
TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.

Species Reactivity
human

Applications
Formats of immunological tests: Protein fragment complementation; Western blot; Functional studies

Product Property

Purity
>95%, by SDS-PAGE, under reducing conditions

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
VCAM1; vascular cell adhesion molecule 1; CD106; INCAM-100; vascular cell adhesion protein 1; CD106 antigen

Gene ID
7412

UniProt ID
P19320

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Related Signaling Pathways

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - IAB-B067(T).pdf

MSDS

MSDS - IAB-B067(T).pdf

COA

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