Recombinant Anti-human VCAM-1 Intrabody [(D-Arg)₉]

Name: Recombinant Anti-human VCAM-1 Intrabody [(D-Arg)9]
SKU: IAB-B067(A)
Rating: 5 (1 reviews)

CAT#: IAB-B067(A)

Recombinant anti-VCAM-1 intrabody linked to (D-Arg)₉ works within cells to bind human VCAM-1, expressed in CHO cells.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to cell junctions.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69867/1706_F12_18_cr57d839e75f94b_selected.jpg

Normal Tissue Figure 2 Kidney

Bowman's capsule Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: Medium Intensity: Strong Quantity: <25%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/154/2835_A_8_5.jpg

Normal Tissue Figure 3 Testis

Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/154/2835_A_6_6.jpg

Normal Tissue Figure 4 Lymph node

Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/154/2835_A_8_8.jpg

Normal Tissue Figure 5 High expression in spleen

Cells in red pulp Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in white pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/154/2835_B_7_4.jpg

RNA Expression Figure 6 RNA cell line category: Group enriched (HHSteC, HSkMC)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162692-VCAM1

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Specifications

Immunogen
human vascular cell adhesion molecule 1

Host Species
mouse

Type
scFv-(D-Arg)₉

Cell Penetrating Peptide
(D-Arg)₉
It has been proven that polyarginine peptides composed of nine arginine amino acids (Arg-9) are able to translocate across cells very efficiently. This raises the question if molecular dynamics simulations would show a similar pathway for this highly hydrophilic and charged peptide as predicted for the Tat peptide. The simplicity of Arg-9 over other CPPs also helps isolate the role of the Arg sidechain on the translocation without the effect of other sidechains. Therefore, in this work we study computationally the translocation of Arg-9 through a lipid bilayer and show that the translocation mechanism is the same as for the Tat peptide.

Species Reactivity
human

Applications
Products can be used for: Fluorescence-activated cell sorting; Enzyme-linked Immunosorbent Assay; Immunofluorescence; Functional studies

Product Property

Purity
>95% as determined by analysis by RP-HPLC

Storage
Store at – 20 or -70°C upon receipt. Divide antibody into aliquots prior usage and avoid multiple freeze-thaw cycles.

Target

Alternative Names
VCAM1; vascular cell adhesion molecule 1; CD106; INCAM-100; vascular cell adhesion protein 1; CD106 antigen

Gene ID
7412

UniProt ID
P19320

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Related Signaling Pathways

TNF Signaling Pathway

Related Diseases

AGE-RAGE Signaling Pathway in Diabetic Complications

Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - IAB-B067(A).pdf

MSDS

MSDS - IAB-B067(A).pdf

COA

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Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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