Is this anti-VEGF recombinant antibody clone B20-4 effective in ELISA?

Yes, the anti-VEGF recombinant antibody clone B20-4 (PABC-131) is effective in ELISA applications. It has been validated for use in such assays and provides reliable detection of VEGF.

What is the appropriate concentration of anti-VEGF recombinant antibody clone B20-4 for use in IHC?

The appropriate concentration of the anti-VEGF recombinant antibody clone B20-4 (PABC-131) for IHC is typically in the range of 1-10 µg/mL. However, it is recommended to optimize the concentration based on the specific conditions and samples used in the experiment.

Does the anti-VEGF recombinant antibody clone B20-4 cross-react with other species?

The anti-VEGF recombinant antibody clone B20-4 (PABC-131) is known to cross-react with VEGF from various species, including human, mouse, and rat. This cross-reactivity makes it a versatile tool for research involving different model organisms.

Can the anti-VEGF recombinant antibody clone B20-4 be used in flow cytometry analysis?

Yes, the anti-VEGF recombinant antibody clone B20-4 (PABC-131) can be used in flow cytometry analysis. It provides specific binding to VEGF, allowing for the detection of VEGF-expressing cells in flow cytometry assays.

What are the recommended storage conditions for the anti-VEGF recombinant antibody clone B20-4?

The recommended storage condition for the anti-VEGF recombinant antibody clone B20-4 (PABC-131) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.

Human Anti-VEGF Recombinant Antibody (clone B20-4)

CAT#: PABC-131

Recombinant Human Antibody (B20-4) is capable of binding to VEGF, expressed in HEK293 cells. Expressed as the combination of a heavy chain (HC) containing VH from antiVEGF mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from antiVEGF mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody binds and blocks both human and murine vascular endothelial growth factor (VEGF).

Tested Data Published Data

Figure 1 Human Anti-VEGF Recombinant Antibody (PABC-131) in WB

Western blot analysis of PABC-131 was performed by loading VEGF165 Protein, Human / Cynomolgus, Recombinant.
PABC-131 incubation concentration: 2ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Lane 1: Reducing Antigen (0.1μg)
Lane 2: Reducing Antigen (0.3μg)
Lane 3: Reducing Antigen (0.6μg)

ELISA

Figure 2 Human Anti-VEGF Recombinant Antibody (PABC-131) in ELISA

ELISA analysis of PABC-131 was performed by coating with VEGF165 Protein, Human / Cynomolgus, Recombinant.

The secondary antibody: HRP-Anti-Human IgG (H+L)

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ELISA

Figure 1 binding of G6-31 and B20-4.1 as IgG (10 μg/ml) to ELISA wells coated with VEGF homologues is shown.

Liang, W. C., Wu, X., Peale, F. V., Lee, C. V., Meng, Y. G., Gutierrez, J., ... & Fuh, G. (2006). Cross-species vascular endothelial growth factor (VEGF)-blocking antibodies completely inhibit the growth of human tumor xenografts and measure the contribution of stromal VEGF. Journal of Biological Chemistry, 281(2), 951-961.

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Specifications

Immunogen
Human Vascular endothelial growth factor

Host Species
Human

Type
Human IgG

Specificity
Human VEGF

Species Reactivity
Human, Mouse

Clone
B20-4

Applications
ELISA, Neut, Block

Product Property

Purification
Protein A/G Purified

Purity
>95% as determined by SDS-PAGE

Concentration
See the COA

Buffer
PBS

Storage
Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Applications

Application Notes
This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Neutralization, Blocking.

Target

Alternative Names
Vascular endothelial growth factor; VEGF

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Breast cancer biomarkers at key points during disease progression

Michael Brown

Effective for Western Blots

The Anti-VEGF Recombinant Antibody (clone B20-4) has been a fantastic addition to our Western blot assays. Its specificity and sensitivity are top-notch, consistently providing clear results. This antibody has become essential for our experiments.

Jessica Davis

Essential for Immunoprecipitation

This antibody has been a key addition to our Western blot and immunoprecipitation assays. The detection of VEGF is always precise and clear, which has allowed us to produce consistent results without the need to worry about variability. Its performance has greatly enhanced our research quality.

David Martinez

Consistent in Flow Applications

We've used this antibody in several flow cytometry and immunofluorescence applications. The consistent high-quality results and strong affinity for VEGF make it a crucial part of our lab's toolkit. The reproducibility has been excellent, making our complex assays more reliable and effective.

Q&As

Is this anti-VEGF recombinant antibody clone B20-4 effective in ELISA?

A: Yes, the anti-VEGF recombinant antibody clone B20-4 (PABC-131) is effective in ELISA applications. It has been validated for use in such assays and provides reliable detection of VEGF.
What is the appropriate concentration of anti-VEGF recombinant antibody clone B20-4 for use in IHC?

A: The appropriate concentration of the anti-VEGF recombinant antibody clone B20-4 (PABC-131) for IHC is typically in the range of 1-10 µg/mL. However, it is recommended to optimize the concentration based on the specific conditions and samples used in the experiment.
Does the anti-VEGF recombinant antibody clone B20-4 cross-react with other species?

A: The anti-VEGF recombinant antibody clone B20-4 (PABC-131) is known to cross-react with VEGF from various species, including human, mouse, and rat. This cross-reactivity makes it a versatile tool for research involving different model organisms.
Can the anti-VEGF recombinant antibody clone B20-4 be used in flow cytometry analysis?

A: Yes, the anti-VEGF recombinant antibody clone B20-4 (PABC-131) can be used in flow cytometry analysis. It provides specific binding to VEGF, allowing for the detection of VEGF-expressing cells in flow cytometry assays.
What are the recommended storage conditions for the anti-VEGF recombinant antibody clone B20-4?

A: The recommended storage condition for the anti-VEGF recombinant antibody clone B20-4 (PABC-131) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.

View the frequently asked questions answered by Creative Biolabs Support.

Citations

Schiffmann, Lars Mortimer, et al. "Tumour-infiltrating neutrophils counteract anti-VEGF therapy in metastatic colorectal cancer." British journal of cancer 120.1 (2019): 69-78. https://doi.org/10.1038/s41416-018-0198-3

This study investigates the impact of tumour-infiltrating neutrophils on the efficacy of anti-VEGF therapy in metastatic colorectal cancer. It highlights that the presence of CD177+ neutrophils in tumors is an adverse prognostic factor for patients treated with bevacizumab, an antibody targeting vascular endothelial growth factor (VEGF). The study demonstrates that a novel bi-specific anti-VEGF/anti-Ang2 compound, BI-880, can overcome resistance to VEGF inhibition by reducing hypoxia and improving vascular normalization in experimental models.
At Creative Biolabs, we are proud to have contributed to this important research by providing the anti-VEGF antibody B20 used in the syngeneic tumor models. Our role was crucial in evaluating the efficacy of BI-880 in combination with VEGF inhibition. This study underscores the potential of combined VEGF/Ang2 inhibition as a promising strategy to enhance treatment outcomes for colorectal cancer patients, highlighting our commitment to advancing cancer research and innovative therapeutic solutions.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# PABC-131, RRID: AB_3111578)

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - PABC-131.pdf

MSDS

MSDS - PABC-131.pdf

COA

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