Human Anti-VEGF Recombinant Antibody (clone B20-4)
CAT#: PABC-131
Recombinant Human Antibody (B20-4) is capable of binding to VEGF, expressed in HEK293 cells. Expressed as the combination of a heavy chain (HC) containing VH from antiVEGF mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from antiVEGF mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody binds and blocks both human and murine vascular endothelial growth factor (VEGF).
Published Data
Tested Data
ELISA
Figure 1 binding of G6-31 and B20-4.1 as IgG (10 μg/ml) to ELISA wells coated with VEGF homologues is shown.
Liang, W. C., Wu, X., Peale, F. V., Lee, C. V., Meng, Y. G., Gutierrez, J., ... & Fuh, G. (2006). Cross-species vascular endothelial growth factor (VEGF)-blocking antibodies completely inhibit the growth of human tumor xenografts and measure the contribution of stromal VEGF. Journal of Biological Chemistry, 281(2), 951-961.
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WB
Figure 1 Human Anti-VEGF Recombinant Antibody (PABC-131) in WB
Western blot analysis of PABC-131 was performed by loading VEGF165 Protein, Human / Cynomolgus, Recombinant.
PABC-131 incubation concentration: 2ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Lane 1: Reducing Antigen (0.1μg)
Lane 2: Reducing Antigen (0.3μg)
Lane 3: Reducing Antigen (0.6μg)
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Specifications
- Immunogen
- Human Vascular endothelial growth factor
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human VEGF
- Species Reactivity
- Human, Mouse
- Clone
- B20-4
- Applications
- ELISA, Neut, Block
Product Property
- Purification
- Protein A/G Purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- See the COA
- Buffer
- PBS
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Neutralization, Blocking.
Target
- Alternative Names
- Vascular endothelial growth factor; VEGF
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Customer Review
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Michael Brown10.Mar,24
Effective for Western Blots
The Anti-VEGF Recombinant Antibody (clone B20-4) has been a fantastic addition to our Western blot assays. Its specificity and sensitivity are top-notch, consistently providing clear results. This antibody has become essential for our experiments.
Jessica Davis18.May,23
Essential for Immunoprecipitation
This antibody has been a key addition to our Western blot and immunoprecipitation assays. The detection of VEGF is always precise and clear, which has allowed us to produce consistent results without the need to worry about variability. Its performance has greatly enhanced our research quality.
David Martinez27.Sep,22
Consistent in Flow Applications
We've used this antibody in several flow cytometry and immunofluorescence applications. The consistent high-quality results and strong affinity for VEGF make it a crucial part of our lab's toolkit. The reproducibility has been excellent, making our complex assays more reliable and effective.
Q&As
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Is this anti-VEGF recombinant antibody clone B20-4 effective in ELISA?
A: Yes, the anti-VEGF recombinant antibody clone B20-4 (PABC-131) is effective in ELISA applications. It has been validated for use in such assays and provides reliable detection of VEGF.
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What is the appropriate concentration of anti-VEGF recombinant antibody clone B20-4 for use in IHC?
A: The appropriate concentration of the anti-VEGF recombinant antibody clone B20-4 (PABC-131) for IHC is typically in the range of 1-10 µg/mL. However, it is recommended to optimize the concentration based on the specific conditions and samples used in the experiment.
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Does the anti-VEGF recombinant antibody clone B20-4 cross-react with other species?
A: The anti-VEGF recombinant antibody clone B20-4 (PABC-131) is known to cross-react with VEGF from various species, including human, mouse, and rat. This cross-reactivity makes it a versatile tool for research involving different model organisms.
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Can the anti-VEGF recombinant antibody clone B20-4 be used in flow cytometry analysis?
A: Yes, the anti-VEGF recombinant antibody clone B20-4 (PABC-131) can be used in flow cytometry analysis. It provides specific binding to VEGF, allowing for the detection of VEGF-expressing cells in flow cytometry assays.
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What are the recommended storage conditions for the anti-VEGF recombinant antibody clone B20-4?
A: The recommended storage condition for the anti-VEGF recombinant antibody clone B20-4 (PABC-131) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.
View the frequently asked questions answered by Creative Biolabs Support.
Citations
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Schiffmann, Lars Mortimer, et al. "Tumour-infiltrating neutrophils counteract anti-VEGF therapy in metastatic colorectal cancer." British journal of cancer 120.1 (2019): 69-78. https://doi.org/10.1038/s41416-018-0198-3This study investigates the impact of tumour-infiltrating neutrophils on the efficacy of anti-VEGF therapy in metastatic colorectal cancer. It highlights that the presence of CD177+ neutrophils in tumors is an adverse prognostic factor for patients treated with bevacizumab, an antibody targeting vascular endothelial growth factor (VEGF). The study demonstrates that a novel bi-specific anti-VEGF/anti-Ang2 compound, BI-880, can overcome resistance to VEGF inhibition by reducing hypoxia and improving vascular normalization in experimental models.
At Creative Biolabs, we are proud to have contributed to this important research by providing the anti-VEGF antibody B20 used in the syngeneic tumor models. Our role was crucial in evaluating the efficacy of BI-880 in combination with VEGF inhibition. This study underscores the potential of combined VEGF/Ang2 inhibition as a promising strategy to enhance treatment outcomes for colorectal cancer patients, highlighting our commitment to advancing cancer research and innovative therapeutic solutions.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABC-131, RRID: AB_3111578)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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