Human Anti-VEGF Recombinant Antibody (clone B20-4) (CAT#: PABC-131)
Recombinant Human Antibody (B20-4) is capable of binding to VEGF, expressed in HEK293 cells. Expressed as the combination of a heavy chain (HC) containing VH from antiVEGF mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from antiVEGF mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody binds and blocks both human and murine vascular endothelial growth factor (VEGF).
Specific Inquiry
ELISA
Figure 1 binding of G6-31 and B20-4.1 as IgG (10 μg/ml) to ELISA wells coated with VEGF homologues is shown.
Liang, W. C., Wu, X., Peale, F. V., Lee, C. V., Meng, Y. G., Gutierrez, J., ... & Fuh, G. (2006). Cross-species vascular endothelial growth factor (VEGF)-blocking antibodies completely inhibit the growth of human tumor xenografts and measure the contribution of stromal VEGF. Journal of Biological Chemistry, 281(2), 951-961.
Specifications
- Immunogen
- Human Vascular endothelial growth factor
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human VEGF
- Species Reactivity
- Human, Mouse
- Clone
- B20-4
- Applications
- ELISA, Neut, Block
Product Property
- Purification
- Protein A/G Purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- See the COA
- Buffer
- PBS
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Neutralization, Blocking.
Target
- Alternative Names
- Vascular endothelial growth factor; VEGF
Related Resources
Bladder Cancer
Rheumatoid Arthritis
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone B20-4"
See other products for "VEGF"
Select a product category from the dropdown menu below to view related products.
Please select product type
ScFv Antibody Products
Humanized Antibody Products
Human Antibody Products
Chimeric Antibody Products
Mouse Antibody Products
Fc Glycosylation Antibody Products
Deglycosylated Antibody Products
Rabbit Monoclonal Antibody Products
IgG Antibody Products
Fab Antibody Products
Single Domain Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-1036-S(P) | Recombinant Human Anti-VEGF Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
| HPAB-1377LY-S(P) | Mouse Anti-VEGF Recombinant Antibody; scFv Fragment (HPAB-1377LY-S(P)) | ELISA, Inhib | Mouse scfv |
| HPAB-J0264-YC-S(P) | Human Anti-VEGF Recombinant Antibody (clone G12); scFv Fragment | ELISA, Neut | Human scFv |
| HPAB-J0265-YC-S(P) | Human Anti-VEGF Recombinant Antibody (clone E9); scFv Fragment | ELISA, Neut | Human scFv |
| HPAB-J0269-YC-S(P) | Mouse Anti-VEGF Recombinant Antibody (clone EGF165); scFv Fragment | ELISA, WB | Mouse scFv |
Customer Reviews and Q&As
Customer Review
Q&As
-
Effective for Western Blots
The Anti-VEGF Recombinant Antibody (clone B20-4) has been a fantastic addition to our Western blot assays. Its specificity and sensitivity are top-notch, consistently providing clear results. This antibody has become essential for our experiments. -
Essential for Immunoprecipitation
This antibody has been a key addition to our Western blot and immunoprecipitation assays. The detection of VEGF is always precise and clear, which has allowed us to produce consistent results without the need to worry about variability. Its performance has greatly enhanced our research quality. -
Consistent in Flow Applications
We've used this antibody in several flow cytometry and immunofluorescence applications. The consistent high-quality results and strong affinity for VEGF make it a crucial part of our lab's toolkit. The reproducibility has been excellent, making our complex assays more reliable and effective.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABC-131, RRID: AB_3111578)
Copy citation
Popular products with customers
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: FC, IHC, FuncS, Inhib, Cyt
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, ELISA, FuncS, IB, FC, SPR, Apop
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, IHC, WB
Application: ELISA, Neut, FuncS
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
