Anti-Human VEGF Recombinant Antibody (FD006) (CAT#: TAB-0346CL)

• Host Species
• Human

• Type
• Human antibody

• Specificity
• Human

• Clone
• FD006

• Applications
• ELISA, PK, Inhib, IHC, WB

• Related Disease
• Colon cancer, tumor, angiogenesis

• Application Notes
• The VEGF antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Pharmacokinetic, Inhibition, Immunohistochemistry, Western Blot.
  ELISA: ELISA plates were coated with 100 μl/well 0.5 μg/ml VEGF. Bevacizumab or FD006 were diluted (10, 2, 0.4, 0.08, 0.016 and 0.0032 μg/ml) and added for 2 hours' incubation in 37. After three times of washing, the Peroxidase-conjugated affinipure goat anti-human immunoglobulin G (IgG) was added as the secondary antibody for 45 minutes at room temperature. Binding signals were visualized using o-phenylenediamine dihydrochloride (OPD) substrate and the light absorbance was measured with an ELISA reader at 492 nm.
  Immunohistochemistry: The rat eyeballs were harvested and fixed in 4% paraformaldehyde. The corneas were excised, embedded in paraffin, cut into 5-μm sections and then prepared for hematoxylin and eosin (H&E) staining. The cornea sections were fixed in acetone. The primary antibodies were VEGF-A, fms-like tyrosine kinase (VEGFR-1, Flt-1) and kinase insert domain receptor (VEGFR-2, Flk-1), matrix metalloproteinase-9 (MMP-9) and intercellular adhesion molecule-1 (ICAM-1). Goat anti-mouse IgG was used as the secondary antibody. Then stained samples were observed and photographed using inverted microscopy.
  Western Blot: The rats were euthanized, and the corneas were harvested from the treated eyes. The corneas were dissected and frozen at -70°C, then homogenized in ice-cold RIPA lysis buffer solution. After being centrifuged for 5 minutes at 12,000 revolutions per minute (rpm), the supernatants of the samples were collected. Certain amounts of the protein were separated by electrophoresis on an 8% and 12% sodium dodecyl sulfate-polyacrylamide gel, and transferred to nitrocellulose membranes. Then the membranes were blocked using 5% skim milk in TBST (20 mM Tris, 150 mM NaCl and 0.1% Tween-20) and incubated overnight with primary antibodies: anti-VEGF, MMP-9, VEGF receptor 1, ICAM-1 and VEGF receptor 2. The secondary polyclonal antibodies were used to detect bound primary antibodies: anti-mouse, anti-rabbit, and anti-goat. After washing, an enhanced chemiluminescence solution were used to visualize specific lanes.

• Alternative Names
• VEGF; Vascular endothelial growth factor

• TAB-0346CL-S(P)
• Anti-Human VEGF Recombinant Antibody scFv Fragment (FD006)

• TAB-0346CL-F(E)
• Anti-Human VEGF Recombinant Antibody Fab Fragment (FD006)

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