The anti-VEGF antibody specifically target VEGF which is the most significant positive regulator of angiogenesis. The specific antibody has good biological activity, and it can inhibit vascular endothelial cell proliferation, tumor growth and corneal neovascularization. Thus the antibody can be used for the preparation of antigen-specific drugs and angiogenesis inhibiting drugs.
Figure 1 Antigen binding identification of FD006 to bind VEGF.
The binding activity of FD006 by ELISA.
Wang, Q., Yang, J., Tang, K., Luo, L., Wang, L., Tian, L., ... & Lv, M. (2014). Pharmacological characteristics and efficacy of a novel anti-angiogenic antibody FD006 in corneal neovascularization. BMC biotechnology, 14(1), 17.
Figure 2 Antigen binding identification of FD006 to bind VEGF.
Affinity detection of FD006 by binding kinetics assays.
Wang, Q., Yang, J., Tang, K., Luo, L., Wang, L., Tian, L., ... & Lv, M. (2014). Pharmacological characteristics and efficacy of a novel anti-angiogenic antibody FD006 in corneal neovascularization. BMC biotechnology, 14(1), 17.
Figure 3 Antigen binding identification of FD006 to bind VEGF.
The inhibitory effects of FD006 on VEGF-induced human umbilical vein endothelial cell (HUVEC) proliferation.
Wang, Q., Yang, J., Tang, K., Luo, L., Wang, L., Tian, L., ... & Lv, M. (2014). Pharmacological characteristics and efficacy of a novel anti-angiogenic antibody FD006 in corneal neovascularization. BMC biotechnology, 14(1), 17.
Figure 4 Hematoxylin and eosin (HE) staining observation of corneas.
At day 7, corneal neovascularization of various sizes (black arrowhead) were present in the superficial central corneal stroma after alkali burn (×200) and neovascularization containing RBCs in the corneal stroma were indicated with black arrowheads. Cornea stroma exhibited edema and mononuclear inflammatory response. In this plane of section, the corneal epithelium is relatively normal. Bar = 100 μm.
Wang, Q., Yang, J., Tang, K., Luo, L., Wang, L., Tian, L., ... & Lv, M. (2014). Pharmacological characteristics and efficacy of a novel anti-angiogenic antibody FD006 in corneal neovascularization. BMC biotechnology, 14(1), 17.
Figure 5 The protein expression of neovascularization-related signal molecules in the cornea.
β-actin served as an internal standard. The protein expression of VEGF, MMP-9, VEGFR-1, VEGFR-2 and ICAM-1 in the cornea was significantly weaker in antibody- or dexamethasone- treated samples.
Wang, Q., Yang, J., Tang, K., Luo, L., Wang, L., Tian, L., ... & Lv, M. (2014). Pharmacological characteristics and efficacy of a novel anti-angiogenic antibody FD006 in corneal neovascularization. BMC biotechnology, 14(1), 17.
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• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
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• Animal-free production
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CAT | Product Name | Application | Type |
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MOB-1036-F(E) | Recombinant Anti-VEGF Antibody Fab Fragment | RIA, IF, FuncS | Fab |
MHH-1036-F(E) | Recombinant Human Anti-VEGF Antibody Fab Fragment | ELISA, IF, FuncS | Fab |
HPAB-0746LY-F(E) | Human Anti-VEGF Recombinant Antibody; Fab Fragment (HPAB-0746LY-F(E)) | ELISA | Humanized Fab |
HPAB-1377LY-F(E) | Mouse Anti-VEGF Recombinant Antibody; Fab Fragment (HPAB-1377LY-F(E)) | ELISA, Inhib | Mouse Fab |
HPAB-0307LY-F(E) | Human Anti-VEGF Recombinant Antibody (clone XPA.10.072); Fab Fragment | ELISA, Inhib | Human Fab2 |
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