Does this antibody have biological activity?

Yes, the anti-VEGF antibody (clone FD006) has good biological activity. It can inhibit vascular endothelial cell proliferation and corneal neovascularization. We have validated it for Inhibition (Inhib), Pharmacokinetic (PK), ELISA, IHC, and WB.

What is the immunogen and species reactivity?

This is a fully Human recombinant antibody (IgG) targeting human VEGF. It was generated to specifically bind VEGF, a key regulator of angiogenesis. It is suitable for use in preparation of antigen-specific drugs and angiogenesis inhibiting research.

Anti-Human VEGF Recombinant Antibody (FD006)

CAT#: TAB-0346CL

The anti-VEGF antibody specifically target VEGF which is the most significant positive regulator of angiogenesis. The specific antibody has good biological activity, and it can inhibit vascular endothelial cell proliferation, tumor growth and corneal neovascularization. Thus the antibody can be used for the preparation of antigen-specific drugs and angiogenesis inhibiting drugs.

Published Data

ELISA

Figure 1 Antigen binding identification of FD006 to bind VEGF.

The binding activity of FD006 by ELISA.

Wang, Q., Yang, J., Tang, K., Luo, L., Wang, L., Tian, L., ... & Lv, M. (2014). Pharmacological characteristics and efficacy of a novel anti-angiogenic antibody FD006 in corneal neovascularization. BMC biotechnology, 14(1), 17.

Figure 2 Antigen binding identification of FD006 to bind VEGF.

Affinity detection of FD006 by binding kinetics assays.

Inhib

Figure 3 Antigen binding identification of FD006 to bind VEGF.

The inhibitory effects of FD006 on VEGF-induced human umbilical vein endothelial cell (HUVEC) proliferation.

IHC

Figure 4 Hematoxylin and eosin (HE) staining observation of corneas.

At day 7, corneal neovascularization of various sizes (black arrowhead) were present in the superficial central corneal stroma after alkali burn (×200) and neovascularization containing RBCs in the corneal stroma were indicated with black arrowheads. Cornea stroma exhibited edema and mononuclear inflammatory response. In this plane of section, the corneal epithelium is relatively normal. Bar = 100 μm.

Figure 5 The protein expression of neovascularization-related signal molecules in the cornea.

β-actin served as an internal standard. The protein expression of VEGF, MMP-9, VEGFR-1, VEGFR-2 and ICAM-1 in the cornea was significantly weaker in antibody- or dexamethasone- treated samples.

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Specifications

Host Species
Human

Type
Human antibody

Specificity
Human

Clone
FD006

Applications
ELISA, PK, Inhib, IHC, WB

Related Disease
Colon cancer, tumor, angiogenesis

Applications

Application Notes
The VEGF antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Pharmacokinetic, Inhibition, Immunohistochemistry, Western Blot.
ELISA: ELISA plates were coated with 100 μl/well 0.5 μg/ml VEGF. Bevacizumab or FD006 were diluted (10, 2, 0.4, 0.08, 0.016 and 0.0032 μg/ml) and added for 2 hours' incubation in 37. After three times of washing, the Peroxidase-conjugated affinipure goat anti-human immunoglobulin G (IgG) was added as the secondary antibody for 45 minutes at room temperature. Binding signals were visualized using o-phenylenediamine dihydrochloride (OPD) substrate and the light absorbance was measured with an ELISA reader at 492 nm.
Immunohistochemistry: The rat eyeballs were harvested and fixed in 4% paraformaldehyde. The corneas were excised, embedded in paraffin, cut into 5-μm sections and then prepared for hematoxylin and eosin (H&E) staining. The cornea sections were fixed in acetone. The primary antibodies were VEGF-A, fms-like tyrosine kinase (VEGFR-1, Flt-1) and kinase insert domain receptor (VEGFR-2, Flk-1), matrix metalloproteinase-9 (MMP-9) and intercellular adhesion molecule-1 (ICAM-1). Goat anti-mouse IgG was used as the secondary antibody. Then stained samples were observed and photographed using inverted microscopy.
Western Blot: The rats were euthanized, and the corneas were harvested from the treated eyes. The corneas were dissected and frozen at -70°C, then homogenized in ice-cold RIPA lysis buffer solution. After being centrifuged for 5 minutes at 12,000 revolutions per minute (rpm), the supernatants of the samples were collected. Certain amounts of the protein were separated by electrophoresis on an 8% and 12% sodium dodecyl sulfate-polyacrylamide gel, and transferred to nitrocellulose membranes. Then the membranes were blocked using 5% skim milk in TBST (20 mM Tris, 150 mM NaCl and 0.1% Tween-20) and incubated overnight with primary antibodies: anti-VEGF, MMP-9, VEGF receptor 1, ICAM-1 and VEGF receptor 2. The secondary polyclonal antibodies were used to detect bound primary antibodies: anti-mouse, anti-rabbit, and anti-goat. After washing, an enhanced chemiluminescence solution were used to visualize specific lanes.

Target

Alternative Names
VEGF; Vascular endothelial growth factor

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Breast cancer biomarkers at key points during disease progression

Inhibits Angiogenesis Effectively

This anti-VEGF antibody (FD006) demonstrated significant inhibition of vascular endothelial cell proliferation in our assays. It is a potent tool for studying angiogenesis. We also found it very effective in Immunohistochemistry for visualizing VEGF distribution in tissues.

Validated for Pharmacokinetics

We used this human antibody for pharmacokinetic (PK) studies and ELISA. The binding was specific and dose-dependent. The provided validation data was very helpful, and the antibody performed exactly as described in our own independent experiments.

Q&As

Does this antibody have biological activity?

A: Yes, the anti-VEGF antibody (clone FD006) has good biological activity. It can inhibit vascular endothelial cell proliferation and corneal neovascularization. We have validated it for Inhibition (Inhib), Pharmacokinetic (PK), ELISA, IHC, and WB.
What is the immunogen and species reactivity?

A: This is a fully Human recombinant antibody (IgG) targeting human VEGF. It was generated to specifically bind VEGF, a key regulator of angiogenesis. It is suitable for use in preparation of antigen-specific drugs and angiogenesis inhibiting research.

View the frequently asked questions answered by Creative Biolabs Support.

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Related Diseases

Bladder Cancer

Rheumatoid Arthritis

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-0346CL.pdf

MSDS

MSDS - TAB-0346CL.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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