How is the scFv linked to the cytokine in this product?

The Anti-mesothelin scFv is fused to the C terminus of the p35 subunit of IL12 through a short linker (GSADGG). This design ensures the immunocytokine retains the ability to bind mesothelin as well as the biological activity of IL12.

How effective is this product in tumor models?

In research, this immunocytokine significantly inhibited human malignant mesothelioma (NCI-H226) grown in the peritoneal cavity of nude mice. It showed anti-tumor activity comparable to that of the SS1P immunotoxin and is designed for treating mesothelin-positive tumors.

Anti-mesothelin Antibody-Cytokine Fusion Protein, IL12-scFv (SS1)

CAT#: ACFP-SH061

This antibody-cytokine fusion protein was achieved by conjugating/fusing the Anti-mesothelin scFv to the C terminus of the p35 subunit of IL12 through a short linker (GSADGG). It was expressed in CHO and purified with affinity chromatography. The immunocytokine retains the ability to bind the mesothelin as well as the biological activity of IL12. The immunocytokine retained sufficient bioactivity of IL12 and significantly inhibited human malignant mesothelioma (NCI-H226) grown in the peritoneal cavity of nude mice and showed comparable anti-tumor activity to that of the SS1P immunotoxin. This immunocytokine was designed for treating mesothelin-positive tumors.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line HeLa

Immunofluorescent staining of human cell line HeLa shows localization to vesicles.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17172/1276_A4_4_selected.jpg

Normal Tissue Figure 2 Fallopian tube

Ciliated cells (cilia axoneme)
Staining:

High

Intensity: Strong

Ciliated cells (tip of cilia)
Staining:

High

Intensity: Strong

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2216/6076_A_7_7.jpg

RNA Expression Figure 3 RNA cell line category: Cell line enhanced (CAPAN-2, EFO-21, HeLa, hTCEpi, OE19)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000102854-MSLN

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Specifications

Target
mesothelin

Molecule Class
IL12-scFv

Species Reactivity
Human

Expression Host
CHO

Clone
SS1

Related Disease
Mesothelin-positive tumors

Cytokine 1
IL12

Cytokines 1 Description
Interleukin 12 (IL-12) is an interleukin that is naturally produced by dendritic cells, macrophages, neutrophils, and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation.

Cytokine 1 Species
Human

Applications
This product can be used therapeutically to deliver biologically active ligands to a desired tissue.

Target

Alternative Names
MSLN; mesothelin; MPF; SMRP; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor

Gene ID
10232

UniProt ID
Q13421

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Breast cancer biomarkers at key points during disease progression

Effective Linker Design

The short linker (GSADGG) used to fuse the scFv to the IL12 p35 subunit seems to maintain the stability of the molecule well. We observed significant inhibition in our human malignant mesothelioma models grown in the peritoneal cavity.

Comparable To Immunotoxins

In our comparative studies, this immunocytokine showed anti-tumor activity comparable to the SS1P immunotoxin. It retained sufficient IL12 bioactivity to be a potent tool for our research into mesothelin-positive tumor treatments.

Q&As

How is the scFv linked to the cytokine in this product?

A: The Anti-mesothelin scFv is fused to the C terminus of the p35 subunit of IL12 through a short linker (GSADGG). This design ensures the immunocytokine retains the ability to bind mesothelin as well as the biological activity of IL12.
How effective is this product in tumor models?

A: In research, this immunocytokine significantly inhibited human malignant mesothelioma (NCI-H226) grown in the peritoneal cavity of nude mice. It showed anti-tumor activity comparable to that of the SS1P immunotoxin and is designed for treating mesothelin-positive tumors.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

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Datasheet

Datasheet - ACFP-SH061.pdf

MSDS

MSDS - ACFP-SH061.pdf

COA

Certificate of Analysis Lookup

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