Mouse Anti-vWF Recombinant Antibody (clone RU5) (CAT#: PABC-133)

Figure 1 Characterization of VWF-A3 mutants.

Binding of conformation-dependent antibody RU5. VWF was immobilized in microtiter plate wells via polyclonal antibody
DD3. Immobilized VWF was incubated with RU5, and bound RU5 was detected as described under "Experimental Procedures." Wild-type VWF and ΔA3-VWF, which lacks the A3 domain, were used as a positive and negative control, respectively. Binding of RU5 to VWF mutants is expressed as a percentage of wt-VWF binding. Each data point represents the mean ±S.D. of three experiments performed in duplicate.

Romijn, R. A. , Westein, E. , Bouma, B. , Schiphorst, M. E. , Sixma, J. J. , & Lenting, P. J. , et al. (2003). Mapping the collagen-binding site in the von willebrand factor-a3 domain. Journal of Biological Chemistry, 278(17), 15035-15039.

Figure 2 Binding of WT-VWF and VWF mutant proteins to immobilized MMRN1, with or without inhibitors.

Effect of RU5 (2 µg/ml, a VWF A3 domain antibody that inhibits collagen binding) and mIgG (2 µg/ml; negative control) on VWF-MMRN1 binding.

D'Andra, N. P., Tasneem, S., Farndale, R. W., Bihan, D., Sadler, J. E., Sebastian, S., ... & Hayward, C. P. (2016). The functions of the A1A2A3 domains in von Willebrand factor include multimerin 1 binding. Thrombosis and haemostasis, 116(07), 87-95.