Mouse Anti-vWF Recombinant Antibody (clone RU5) (CAT#: PABC-133)
Recombinant Mouse Antibody (RU5) is capable of binding to vWF, expressed in Chinese Hamster Ovary cells (CHO). This antibody inhibits binding of vWF to collagen.
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Custom Production
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ELISA
Figure 1 Characterization of VWF-A3 mutants.
Binding of conformation-dependent antibody RU5. VWF was immobilized in microtiter plate wells via polyclonal antibody DD3. Immobilized VWF was incubated with RU5, and bound RU5 was detected as described under "Experimental Procedures." Wild-type VWF and ΔA3-VWF, which lacks the A3 domain, were used as a positive and negative control, respectively. Binding of RU5 to VWF mutants is expressed as a percentage of wt-VWF binding. Each data point represents the mean ±S.D. of three experiments performed in duplicate.
Romijn, R. A. , Westein, E. , Bouma, B. , Schiphorst, M. E. , Sixma, J. J. , & Lenting, P. J. , et al. (2003). Mapping the collagen-binding site in the von willebrand factor-a3 domain. Journal of Biological Chemistry, 278(17), 15035-15039.
Inhib
Figure 2 Binding of WT-VWF and VWF mutant proteins to immobilized MMRN1, with or without inhibitors.
Effect of RU5 (2 µg/ml, a VWF A3 domain antibody that inhibits collagen binding) and mIgG (2 µg/ml; negative control) on VWF-MMRN1 binding.
D'Andra, N. P., Tasneem, S., Farndale, R. W., Bihan, D., Sadler, J. E., Sebastian, S., ... & Hayward, C. P. (2016). The functions of the A1A2A3 domains in von Willebrand factor include multimerin 1 binding. Thrombosis and haemostasis, 116(07), 87-95.
Specifications
- Immunogen
- Human Von Willebrand Factor
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human vWF
- Species Reactivity
- Human
- Clone
- RU5
- Applications
- ELISA, WB, IHC, IF, Inhib, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody RU5 has been reported in applications of Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence, Inhibition, Functional Assay. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.
Target
- Alternative Names
- VWF; VWD; Von Willebrand Factor; Coagulation Factor VIII VWF; VWF
- Gene ID
- 7450
- UniProt ID
- P04275
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-132 | Mouse Anti-VWF Recombinant Antibody (clone 82D6A3); Fab Fragment | WB, IF, FuncS | Mouse Fab |
| PFBW-132 | Mouse Anti-VWF Recombinant Antibody (clone NMC-4); Fab Fragment | Block | Mouse Fab |
| FAMAB-0297-YC-F(E) | Human Anti-VWF Recombinant Antibody (clone h82D6A3); Fab Fragment | ELISA, WB | Humanized Fab |
| FAMAB-0298-YC-F(E) | Mouse Anti-VWF Recombinant Antibody (clone m-82D6A3); Fab Fragment | ELISA, WB | Mouse Fab |
| HPAB-0343-WJ-F(E) | Mouse Anti-VWF Recombinant Antibody; Fab Fragment (HPAB-0343-WJ-F(E)) | ELISA, WB | Mouse Fab |
Customer Reviews and Q&As
Customer Review
Q&As
Great for IHC
I utilized the Mouse Anti-vWF Recombinant Antibody (clone RU5) for immunohistochemistry on patient tissue samples and was impressed by the clear and specific staining patterns. It's now a staple in my lab for detecting von Willebrand factor.
Reliable for ELISA and WB
The Mouse Anti-vWF Recombinant Antibody (clone RU5) has proven to be quite successful in our cardiovascular studies. Validation across many assays demonstrates its reliability for both ELISA and Western blot applications, which improves our understanding of blood coagulation.
Clear bands and minimal background noise
We chose Mouse Anti-vWF Recombinant Antibody (clone RU5) because of its recombinant nature, which ensures consistency and reliability. Our Western blot gels have never looked better, with consistently distinct bands and minimal background noise.
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For Research Use Only. Not For Clinical Use.
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