GM-CSF-DT immunotoxin

This immunotoxin was achieved by conjugating/fusing the Granulocyte-Macrophage Colony-Stimulating Factor to Diphtheria toxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to malignant or differentiated myeloid cells. Once bind, the toxin enters cells by receptor-mediated endocytosis, then Before the N-terminal 193 amino acids corresponding to the catalytic domain of DT enzyme; -II binding and elongation factor into the eukaryotic cell; the cell can catalyze specific elongation factor EF2 amide His699 of diphtheria ADP2 ribosylation occurs; thereby preventing protein synthesis and causes cell death. It was designed for treatment of acute myeloid leukemia (AML).