Z-domain of protein A scaffold protein anti-Human c-Jun
CAT#: SZA-L217
This c-Jun specific binding protein (Z-domain of protein A) was investigated by an α-helix shuffling strategy. The primary scaffold protein was from a naive combinatorial library of the three-helix bundle Z domain derived from staphylococcal protein A. A hierarchical library was constructed through selective re-randomization of six amino acid positions in one of the two α-helices of the domain, making up the Taq DNA polymerase binding surface. After selections using monovalent phage display technology, second generation variants were identified having affinities (KD=5 mM) for c-Jun as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31174/1313_G11_5_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3801/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows moderate nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3801/10121_A_6_2_selected.jpg
Normal Tissue
Figure 4 IHC staining of human fallopian tube
High expression. RNA expression: 314.1 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7780/18871_A_8_7_val_selected.jpg
Normal Tissue
Figure 5 IHC staining of human lymph node
Low expression. RNA expression: 52.1 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7780/18871_A_8_8_val_selected.jpg
Normal Tissue
Figure 6 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3801/10121_B_7_5.jpg
Normal Tissue
Figure 7 Thyroid gland
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3801/10121_B_2_5.jpg
Normal Tissue
Figure 8 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3801/10121_A_9_3.jpg
Normal Tissue
Figure 9 Liver
Hepatocytes
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3801/10121_A_7_4.jpg
Normal Tissue
Figure 10 Kidney
Distal tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Proximal tubules (cell body)
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3801/10121_A_8_5.jpg
Normal Tissue
Figure 11 Testis
Sertoli cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3801/10121_A_5_6.jpg
RNA Expression
Figure 12 RNA cell line category: Cell line enhanced (RH-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000177606-JUN
❮
❯
Specifications
- Scaffold Name
- Z-domain of protein A
- Origin
- Staphylococcal protein A
- Core Structure
- 3-α helixes
- Variable Regions
- 13 Residues on first and second helix surface
- Target
- c-Jun
- Species Reactivity
- Human
- Expression Host
- BL21(DE3)
- Affinity Constant
- 5 mM
- Applications
- ELISA; IHC; Microscopy; FC; WB; FuncS
Target
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Related Signaling Pathways
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Downloadable Resources
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Datasheet
MSDS
COA
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