Rabbit Anti-ACVRL1 Recombinant Antibody (clone 8B6)
CAT#: ZG-0528J
This product is a rabbit antibody that recognizes ACVRL1.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-ACVRL1 Antibody (ZG-0528J) in WB
Western Blot
Positive WB detected in: Daudi whole cell lysate, U87 whole cell lysate
All lanes: ACVRL1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 57 kDa
Observed band size: 65 kDa
IHC
Figure 2 Rabbit Anti-ACVRL1 Antibody (ZG-0528J) in IHC
IHC image of ZG-0528J diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Normal Tissue
Figure 1 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong granular cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7041/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7041/21213_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7041/21213_A_7_3.jpg
Normal Tissue
Figure 4 Gallbladder
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7041/21213_A_5_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7041/21213_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: Low Intensity: Weak Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7041/21213_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7041/21213_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, EFO-21, HUVEC TERT2, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000139567-ACVRL1
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Specifications
- Immunogen
- A synthesized peptide derived from human ACVRL1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ACVRL1
- Species Reactivity
- Human
- Clone
- 8B6
- Applications
- ELISA, WB, IHC
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200
Target
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Excellent Western Blot
Clone 8B6 is a robust rabbit antibody for detecting ACVRL1. We used it in Western blot at a 1:1000 dilution, and it gave a strong, specific signal for the receptor. The antibody is affinity purified and reacts well with human samples, aiding our TGF-beta signaling research.
Precise IHC Staining
We validated this antibody for immunohistochemistry, using a dilution of 1:100. It provided precise staining of ACVRL1 in tissue sections. The liquid formulation with sodium azide and glycerol is stable at -20°C, and we have had no issues with degradation during storage.
Q&As
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What are the recommended dilutions for WB and IHC?
A: For Western Blot (WB), the recommended dilution is 1:500-1:5000. For Immunohistochemistry (IHC), the dilution is 1:50-1:200. This rabbit antibody (clone 8B6) recognizes Human ACVRL1.
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What immunogen was used to generate this antibody?
A: The antibody was generated using a synthesized peptide derived from human ACVRL1. It is a Recombinant Rabbit Antibody purified by affinity purification and validated for ELISA, WB, and IHC.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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