Rabbit Anti-AFP Recombinant Antibody (clone R04-3C6) (CAT#: VS3-XY3254)
It is a recombinant rabbit antibody targeting the AFP (Alpha fetoprotein), which binds copper, nickel, fatty acids, bilirubin and serum albumin. It has been determined using Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation and Flow cytometry.
Specific Inquiry
Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol & the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10607/1816_H10_5_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol & the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10607/1816_H10_5_selected.jpg
Figure 2 IHC staining of human placenta
(Immunohistochemical staining of human placenta shows strong positivity in plasma.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10607/25809_A_2_7_selected.jpg
(Immunohistochemical staining of human placenta shows strong positivity in plasma.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10607/25809_A_2_7_selected.jpg
Figure 3 RNA cell line category: Cell line enriched (Hep G2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000081051-AFP
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000081051-AFP
Figure 3 RNA cell line category: Cell line enriched (Hep G2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000081051-AFP
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000081051-AFP
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human AFP
- Species Reactivity
- Human
- Clone
- R04-3C6
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Related Disease
- Alpha-Fetoprotein Deficiency
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- AFPD; FETA; HPAFP
- Gene ID
- 174
- UniProt ID
- P02771
- Long Name
- Alpha fetoprotein
- Cellular Localization
- Secreted
- Post Translation Modifications
- Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated.
- Protein Refseq
- NP_001341646.2; NP_001125.1
- Function
- Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-011 | Recombinant Anti-Human AFP VHH Single Domain Antibody | CA, IP, IF, FuncS | Llama VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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