Mouse Anti-ALK Recombinant Antibody (VS-0224-XY7)
CAT#: VS-0224-XY7
The product is a mouse monoclonal antibody, which specifically recognizes ECD region of human ALK (ALK receptor tyrosine kinase). It can be used for Enzyme-linked immunosorbent assay, Immunofluorescence and Immunocytochemistry.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10694/1416_A5_3_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10694/26100_B_8_5.jpg
Normal Tissue
Figure 3 Cerebellum
Cells in molecular layer Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Purkinje cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10694/26100_B_7_8.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10694/26075_A_7_3.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10694/26075_A_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enriched (SH-SY5Y)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171094-ALK
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Specifications
- Immunogen
- Mouse myeloma cell line NS0-derived recombinant human ALK/CD246 (AA 19-1038)
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human ALK
- Species Reactivity
- Human
- Applications
- ELISA, Immunofluorescence, Immunocytochemistry
- Related Disease
- Neuroblastoma
Product Property
- Purification
- Protein A purified
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested in Immunofluorescence and Immunocytochemistry at a concentration of 8-25 ug/mL.
Target
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Related Diseases
EGFR Tyrosine Kinase Inhibitor Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ALK"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1446z | Mouse Anti-ALK Recombinant Antibody (clone 26D9) | WB, FC, ICC, IF | Mouse IgG1, κ |
| HPAB-1817LY | Mouse Anti-ALK Recombinant Antibody (HPAB-1817LY) | ELISA, WB, FC | Mouse IgG |
| HPAB-1818LY | Mouse Anti-ALK Recombinant Antibody (HPAB-1818LY) | ELISA, WB, FC | Mouse IgG |
| HPAB-1819LY | Mouse Anti-ALK Recombinant Antibody (HPAB-1819LY) | ELISA, WB, FC | Mouse IgG |
| VS3-FY2624 | Mouse Anti-ALK/p80 Recombinant Antibody (VS3-FY2624) | WB, FC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0147 | Hi-Affi™ Rabbit Anti-ALK Recombinant Antibody (clone DS147AB) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-004CN | Human Anti-ALK Recombinant Antibody (clone ESBA521) | ELISA, WB, FuncS | Human IgG |
| NS-004CN-F(E) | Human Anti-ALK Recombinant Antibody (clone ESBA521); Fab Fragment | ELISA, WB | Human Fab |
| NS-004CN-S(P) | Human Anti-ALK Recombinant Antibody (clone ESBA521); scFv Fragment | ELISA, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1817LY-F(E) | Mouse Anti-ALK Recombinant Antibody; Fab Fragment (HPAB-1817LY-F(E)) | ELISA, WB, FC | Mouse Fab |
| HPAB-1818LY-F(E) | Mouse Anti-ALK Recombinant Antibody; Fab Fragment (HPAB-1818LY-F(E)) | ELISA, WB, FC | Mouse Fab |
| HPAB-1819LY-F(E) | Mouse Anti-ALK Recombinant Antibody; Fab Fragment (HPAB-1819LY-F(E)) | ELISA, WB, FC | Mouse Fab |
| HPAB-1820LY-F(E) | Mouse Anti-ALK Recombinant Antibody; Fab Fragment (HPAB-1820LY-F(E)) | ELISA, WB, FC | Mouse Fab |
| HPAB-1821LY-F(E) | Mouse Anti-ALK Recombinant Antibody; Fab Fragment (HPAB-1821LY-F(E)) | ELISA, WB, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1817LY-S(P) | Mouse Anti-ALK Recombinant Antibody; scFv Fragment (HPAB-1817LY-S(P)) | ELISA, WB, FC | Mouse scFv |
| HPAB-1818LY-S(P) | Mouse Anti-ALK Recombinant Antibody; scFv Fragment (HPAB-1818LY-S(P)) | ELISA, WB, FC | Mouse scFv |
| HPAB-1819LY-S(P) | Mouse Anti-ALK Recombinant Antibody; scFv Fragment (HPAB-1819LY-S(P)) | ELISA, WB, FC | Mouse scFv |
| HPAB-1820LY-S(P) | Mouse Anti-ALK Recombinant Antibody; scFv Fragment (HPAB-1820LY-S(P)) | ELISA, WB, FC | Mouse scFv |
| HPAB-1821LY-S(P) | Mouse Anti-ALK Recombinant Antibody; scFv Fragment (HPAB-1821LY-S(P)) | ELISA, WB, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC625 | Recombinant Anti-ALK Vesicular Antibody, EV Displayed (VS-0425-YC625) | ELISA, FC, Stim, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY319 | Anti-ALK Immunohistochemistry Kit | IHC |
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