Mouse Anti-ANXA2 Recombinant Antibody (clone 1A1E1)
CAT#: ZG-0185U
Mouse anti-ANXA2 antibody (clone 1A1E1) displayed high specificity against the ANXA2 protein of Human.
Tested Data
Gene Expression
IHC
Figure 2 Mouse Anti-ANXA2 Antibody (ZG-0185U) in IHC
Immunohistochemical of paraffin-embedded Human pancreas tissue using ZG-0185U at dilution of 1:200.
WB
Figure 1 Mouse Anti-ANXA2 Antibody (ZG-0185U) in WB
All Lanes: Mouse anti ANXA2 Monoclonal antibody at 1μg/ml
lane 1: HepG2 whole cell lysate
Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size: 39,41kd
Observed band size: 50KD
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46964/1865_G3_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61798/138247_B_9_5.jpg
Normal Tissue
Figure 3 Lung
Alveolar cells type I Staining: Medium Intensity: Moderate Quantity:>75% Alveolar cells type I Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Macrophages Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61798/138247_A_3_4.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61798/138247_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61798/138247_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61798/138247_A_7_5.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61798/138247_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000182718-ANXA2
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Specifications
- Immunogen
- Recombinant Human ANXA2 protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human ANXA2
- Species Reactivity
- Human
- Clone
- 1A1E1
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Antiphospholipid Syndrome and Upper Tract Urothelial Carcinoma.
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000,IHC:1:50-1:500
Target
- Alternative Names
- P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270; annexin A2; annexin II; annexin-2; calpactin I heavy chain; calpactin I heavy polypeptide; calpactin-1 heavy chain; chromobindin 8; epididymis secretory protein Li 270; epididymis secretory sperm binding protein; lipocortin II; placental anticoagulant protein IV; protein I
- Gene ID
- 302
- UniProt ID
- P07355
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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