Mouse Anti-ANXA2 Recombinant Antibody (clone 16G3)
CAT#: MOB-1394z
This product is a mouse antibody that recognizes human ANXA2. It can be used in the following applications: WB, IP, IHC, IF, FCM, ELISA.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46964/1865_G3_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61798/138247_B_9_5.jpg
Normal Tissue
Figure 3 Lung
Alveolar cells type I Staining: Medium Intensity: Moderate Quantity:>75% Alveolar cells type I Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Macrophages Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61798/138247_A_3_4.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61798/138247_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61798/138247_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61798/138247_A_7_5.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61798/138247_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000182718-ANXA2
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Specifications
- Immunogen
- Human Annexin A2
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human ANXA2
- Species Reactivity
- Human
- Clone
- 16G3
- Applications
- WB, IP, IHC, IF, FC, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- ANXA2; Chromobindin 8; HEL-S-270; Annexin-2; Protein I; P36; Lipocortin II; ANX2L4; Epididymis Secretory Protein Li 270; ANX2; LPC2; Placental Anticoagulant Protein IV; Calpactin I Heavy Polypeptide; Annexin II
- UniProt ID
- P07355
REVIEWS AND Q&AS
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High Immunoprecipitation Efficiency
I achieved high precipitation efficiency with this antibody in immunoprecipitation assays. It specifically binds to the target protein, making the results clear and reliable.
High Performance in ELISA
High performance in ELISA assays, providing clear and quantifiable results.
Effective in Flow Cytometry
Utilized this antibody for flow cytometry with excellent results. The fluorescence intensity was robust, and it helped us achieve clear and distinguishable cell populations.
Q&As
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Does this antibody recognize proteins that are scarce?
A: Indeed, it is quite sensitive; nevertheless, for very low-abundance proteins, more sophisticated detection techniques or signal amplification stages would be necessary.
-
How can I select the ideal lysis buffer for my specimens?
A: Select a lysis buffer (like RIPA buffer) that preserves the integrity of the proteins and works with your downstream applications.
-
Is there a particular buffer that is suggested for reconstitution of antibodies?
A: Reconstitute at pH 7.4 in sterile PBS. To determine the precise volume to use, refer to the datasheet's instructions.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "ANXA2"
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| VS-0325-XY119 | Anti-ANXA2 Immunohistochemistry Kit | IHC | |
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| VS-0525-XY410 | Anti-Human ANXA2 Immunohistochemistry Kit | IHC | |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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