Mouse Anti-APOE Recombinant Antibody (clone 2E8) (CAT#: HPAB-0115CQ)

Figure 1 Immunoreactivity of apoE variants with anti-apoE mAbs 2E8.

Serial dilutions of apoE variants were incubated in polystyrene microwells to which anti-apoE mAb 6C5 had been adsorbed. The wells were then washed and exposed to '251-labeled 2E8 and, after washing, the bound radioactivity was determined. The bound '2SI-labeled mAb mass was calculated from the specific activity of the labeled mAb.

Raffai, R., Maurice, R., Weisgraber, K., Innerarity, T., Wang, X. I. N. G. B. O., MacKenzie, R.,... & Milne, R. (1995). Molecular characterization of two monoclonal antibodies specific for the LDL receptor-binding site of human apolipoprotein E. Journal of lipid research, 36(9), 1905-1918.

Figure 2 Sensorgrams of apoE variants with 1D7,2E8, and 3H1 using SPR.

These sensorgrams depict the kinetics of 1D7, 2E8, and 3H1 binding to apoE3, apoE(Argl36→Ser), apoE(His14O→Ala), apoE(Lys145→Ala), apoE(Arg150→Ala), and apoE(Arg158→Cys). The association phase of each sensogram begins at approximately 180 sec and the dissociation phase at 850 sec. The results that are illustrated were obtained with concentrations of 150 nM, 150 nM, and 50 nM for 1D7,2E8, and 3H1, respectively

Raffai, R., Maurice, R., Weisgraber, K., Innerarity, T., Wang, X. I. N. G. B. O., MacKenzie, R.,... & Milne, R. (1995). Molecular characterization of two monoclonal antibodies specific for the LDL receptor-binding site of human apolipoprotein E. Journal of lipid research, 36(9), 1905-1918.

Figure 1 Mouse Anti-APOE Recombinant Antibody (HPAB-0115CQ) in SDS-PAGE

SDS-PAGE analysis of HPAB-0115CQ in non-reduced and reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 150-200 kDa.