CytoStream™ Rabbit Anti-BAD Recombinant Antibody (VS13-YC83)
CAT#: VS13-YC83
The CytoStream™ Rabbit Anti-BAD Recombinant Antibody is specific for human BAD. BAD encodes a pro-apoptotic BCL-2 family protein that regulates apoptosis through phosphorylation and interaction with proteins like BCL-xL, with alternative splicing variants found. The anti-BAD antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28185/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in subset of cells in germinal and non-germinal center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/62105/ihc_selected.jpg
Normal Tissue
Figure 3 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4205/11845_A_9_8.jpg
Normal Tissue
Figure 4 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4205/11825_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28185/61308_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28185/61308_A_9_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28185/61308_A_8_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000002330-BAD
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Specifications
- Immunogen
- Recombinant human Bad, aa 1-50.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human BAD
- Species Reactivity
- Human
- Applications
- WB, ICC, IF, IHC-P, FC, IP
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500
Immunocytochemistry (ICC): 1/50
Immunofluorescence (IF): 1/50
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50
Target
- Alternative Names
- BCL2 Associated Agonist Of Cell Death; Bcl-XL/Bcl-2-Associated Death Promoter; Bcl2 Antagonist Of Cell Death; Bcl-2-Binding Component 6; Bcl-2-Like Protein 8; Bcl2-L-8; BCL2L8; BCL2-Antagonist Of Cell Death Protein;
- Gene ID
- 572
- UniProt ID
- Q92934
- Long Name
- BCL2 Associated Agonist Of Cell Death
- Cellular Localization
- Mitochondrion outer membrane; Cytoplasm
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Related Signaling Pathways
cAMP Signaling Pathway
ErbB Signaling Pathway
PI3K-Akt Signaling Pathway
Related Diseases
Colorectal Cancer
Endometrial Cancer
Amyotrophic Lateral Sclerosis
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "BAD"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0064MZ | Chicken Anti-BAD Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0317 | Hi-Affi™ Rabbit Anti-BAD Recombinant Antibody (clone DS317AB) | IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1677-CN | Rabbit Anti-BAD Polyclonal Antibody (MRO-1677-CN) | IF, IHC, FC | Rabbit IgG |
| VS3-QX100 | Mouse Anti-BAD Recombinant Antibody (clone 27AT381) | WB | Mouse IgG1 |
| VS3-XY115 | Mouse Anti-BAD Recombinant Antibody (clone 1B1) | ELISA, WB, FC | Mouse IgG1 |
| VS3-XY116 | Mouse Anti-BAD Recombinant Antibody (clone 2H4) | WB, IP, IF, IHC | Mouse IgG1 |
| VS3-XY117 | Mouse Anti-BAD Recombinant Antibody (clone 1G5B3) | ELISA, WB, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-XY1 | Mouse Anti-BAD Agonistic Antibody (VS-0724-XY1) | WB, IHC, ICC | Mouse IgG2a |
| VS-0724-XY2 | Rat Anti-Bad Agonistic Antibody (VS-0724-XY2) | WB, ICC | Rat IgG2a |
| VS-0724-XY3 | Mouse Anti-BAD Agonistic Antibody (VS-0724-XY3) | ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY46 | Rabbit Anti-NHP BAD Recombinant Antibody (VS-1024-XY46) | WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY680 | Anti-BAD Immunohistochemistry Kit | IHC |
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