CytoStream™ Rabbit Anti-BAD Recombinant Antibody (VS13-YC83) (CAT#: VS13-YC83)
The CytoStream™ Rabbit Anti-BAD Recombinant Antibody is specific for human BAD. BAD encodes a pro-apoptotic BCL-2 family protein that regulates apoptosis through phosphorylation and interaction with proteins like BCL-xL, with alternative splicing variants found. The anti-BAD antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28185/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28185/if_selected.jpg
Figure 2 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in subset of cells in germinal and non-germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/62105/ihc_selected.jpg
(Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in subset of cells in germinal and non-germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/62105/ihc_selected.jpg
Figure 3 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4205/11845_A_9_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4205/11845_A_9_8.jpg
Figure 4 Cerebral cortex
(Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4205/11825_B_8_5.jpg
(Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4205/11825_B_8_5.jpg
Figure 5 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28185/61308_A_9_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28185/61308_A_9_3.jpg
Figure 6 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28185/61308_A_9_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28185/61308_A_9_4.jpg
Figure 7 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28185/61308_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28185/61308_A_8_5.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000002330-BAD
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000002330-BAD
Specifications
- Immunogen
- Recombinant human Bad, aa 1-50.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human BAD
- Species Reactivity
- Human
- Applications
- WB, ICC, IF, IHC-P, FC, IP
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500
Immunocytochemistry (ICC): 1/50
Immunofluorescence (IF): 1/50
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50
Target
- Alternative Names
- BCL2 Associated Agonist Of Cell Death; Bcl-XL/Bcl-2-Associated Death Promoter; Bcl2 Antagonist Of Cell Death; Bcl-2-Binding Component 6; Bcl-2-Like Protein 8; Bcl2-L-8; BCL2L8; BCL2-Antagonist Of Cell Death Protein;
- Gene ID
- 572
- UniProt ID
- Q92934
- Long Name
- BCL2 Associated Agonist Of Cell Death
- Cellular Localization
- Mitochondrion outer membrane; Cytoplasm
Recommended Products
Secondary Antibody
Isotype Control
Related Resources
cAMP Signaling Pathway
ErbB Signaling Pathway
PI3K-Akt Signaling Pathway
Colorectal Cancer
Endometrial Cancer
Amyotrophic Lateral Sclerosis
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0064MZ | Chicken Anti-BAD Polyclonal IgY | WB | Chicken antibody |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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