Rabbit Anti-BCL2L1 Recombinant Antibody (clone 5E9) (CAT#: ZG-0119U)
Rabbit anti-BCL2L1 antibody (clone 5E9) displayed high specificity against the BCL2L1 protein of Human, Mouse and Rat.
Specific Inquiry
WB
Figure 1 Rabbit Anti-BCL2L1 Antibody (ZG-0119U) in WB
Positive WB detected in: K562 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate. All lanes: Bcl-XL antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 27, 19, 26 kDa
Observed band size: 30 kDa
IHC
Figure 2 Rabbit Anti-BCL2L1 Antibody (ZG-0119U) in IHC
IHC image of ZG-0119U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human Bcl-XL
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human BCL2L1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 5E9
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Absolute Glaucoma and Tongue Carcinoma
Product Property
- Purification
- Affinity Purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- BCLX; BCL2L; Bcl-X; PPP1R52; BCL-XL/S; bcl-2-like protein 1; apoptosis regulator Bcl-X; protein phosphatase 1, regulatory subunit 52
- Gene ID
- 598
- UniProt ID
- Q07817
Related Resources
Amyotrophic Lateral Sclerosis
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1570z | Mouse Anti-BCL2L1 Recombinant Antibody (clone 24B10) | ELISA, IF, FuncS | Mouse IgG2a |
| MOB-0914MZ | Mouse Anti-BCL2L1 Recombinant Antibody (clone 8C3.6) | FC, WB | Mouse IgG3 |
| MRO-1685-CN | Rabbit Anti-BCL2L1 Polyclonal Antibody (MRO-1685-CN) | WB, IF, IHC | Rabbit IgG |
| ZG-0186U | Mouse Anti-BCL2L1 Recombinant Antibody (clone 3D1C1) | ELISA, WB, IHC | Mouse IgG1 |
| VS3-QX114 | Mouse Anti-BCL2L1 Recombinant Antibody (clone 804CT19.1.4) | WB | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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