Mouse Anti-BMI1 Recombinant Antibody (VS3-CJ101)
CAT#: VS3-CJ101
This product is a mouse antibody that recognizes BMI1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30471/1564_A5_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30472/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in cortical cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/ihc_selected.jpg
Normal Tissue
Figure 4 Testis
Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_6_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_8_8.jpg
Normal Tissue
Figure 6 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_B_8_5.jpg
Normal Tissue
Figure 7 Nasopharynx
Respiratory epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_B_8_2.jpg
Normal Tissue
Figure 8 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_7_3.jpg
Normal Tissue
Figure 9 Kidney
Collecting ducts Staining: Medium Intensity: Strong Quantity: <25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_7_5.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (HDLM-2, U-937)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168283-BMI1
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Specifications
- Immunogen
- Recombinant fragment of human BMI1 (C-terminus)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human BMI1
- Species Reactivity
- Human
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/mL (lot specific)
- Buffer
- 50% Glycerol, 1% BSA, PBS, pH7.4.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in ELISA (1:15000-1:20000), Western Blot (1:100-1:2000).
Target
- Alternative Names
- FLVI2/BMI1; PCGF4; RNF51; flvi-2/bmi-1
- Gene ID
- 648
- UniProt ID
- P35226
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Monoubiquitinated (By similarity).
May be polyubiquitinated; which does not lead to proteasomal degradation.
- Protein Refseq
- NP_001190991.1; NP_005171.4
- Function
- Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:15386022, PubMed:16359901, PubMed:26151332, PubMed:16714294, PubMed:21772249, PubMed:25355358, PubMed:27827373).
The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A (PubMed:21772249, PubMed:25355358).
In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2 (PubMed:15386022, PubMed:26151332, PubMed:21772249).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
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- Product Name
Secondary Antibody
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Recommended Dilution Buffer
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- Product Name
See other products for "BMI1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4024z | Mouse Anti-BMI1 Recombinant Antibody (clone 43H11) | WB, FC, ICC, IF, IHC | Mouse IgG1 |
| ZG-0973F | Mouse Anti-BMI1 Recombinant Antibody (clone 229F6) | IHC-Fr, IP, WB | Mouse IgG1 |
| ZG-0570J | Rabbit Anti-BMI1 Recombinant Antibody (clone 1F2) | ELISA, WB, IHC | Rabbit IgG |
| VS3-CJ895 | Mouse Anti-BMI1 Recombinant Antibody (VS3-CJ895) | WB, ICC | Mouse IgG1, κ |
| VS3-CJ896 | Rabbit Anti-BMI1 Recombinant Antibody (VS3-CJ896) | WB, ICC, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0365 | Hi-Affi™ Rabbit Anti-BMI1 Recombinant Antibody (clone DS365AB) | WB, IHC, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0449 | PE-A*02:01/Human BMI1 (TLQDIVYKL) MHC Tetramer | FCM, IHC | |
| MHC-CN0450 | APC-A*02:01/Human BMI1 (TLQDIVYKL) MHC Tetramer | FCM, IHC | |
| MHC-CN0451 | FITC-A*02:01/Human BMI1 (TLQDIVYKL) MHC Tetramer | FCM, IHC | |
| MHC-YF337 | A*02:01/Human BMI1 (CLPSPSTPV) MHC Pentamer | FCM | |
| MHC-YF338 | A*02:01/Human BMI1 (TLQDIVYKL) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY31 | AbPlus™ Anti-BMI1 Magnetic Beads (229F6) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC21 | Rabbit Anti-BMI1 Antibody (VS-0924-YC21) - Cancer Stem Cell Marker | IF | Rabbit IgG |
| VS-0924-YC22 | Rabbit Anti-BMI1 Antibody (VS-0924-YC22) - Cancer Stem Cell Marker | IHC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY249 | Anti-BMI1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY792 | Anti-Mouse BMI1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY791 | Anti-Human BMI1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC101 | CytoStream™ Mouse Anti-BMI1 Recombinant Antibody (VS13-YC101) | WB, ICC, IF, IHC-P, FC | Mouse IgG2a |
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