Mouse Anti-BMI1 Recombinant Antibody (VS3-CJ22) (CAT#: VS3-CJ22)
This product is a mouse antibody that recognizes BMI1.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30471/1564_A5_1_selected.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30471/1564_A5_1_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30472/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30472/if_selected.jpg
Figure 3 IHC staining of human adrenal gland
(Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in cortical cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/ihc_selected.jpg
(Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in cortical cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/ihc_selected.jpg
Figure 4 Testis
(Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_6_6.jpg
(Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_6_6.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_8_8.jpg
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_8_8.jpg
Figure 6 Cerebral cortex
(Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_B_8_5.jpg
(Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_B_8_5.jpg
Figure 7 Nasopharynx
(Respiratory epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_B_8_2.jpg
(Respiratory epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_B_8_2.jpg
Figure 8 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_7_3.jpg
Figure 9 Kidney
(Collecting ducts Staining: Medium Intensity: Strong Quantity: <25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_7_5.jpg
(Collecting ducts Staining: Medium Intensity: Strong Quantity: <25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_7_5.jpg
Figure 10 RNA cell line category: Cell line enhanced (HDLM-2, U-937)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168283-BMI1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168283-BMI1
Specifications
- Immunogen
- KLH-conjugated synthetic peptide from the N-terminal region of human Bmi1
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human BMI1
- Species Reactivity
- Human
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/mL (lot specific)
- Buffer
- 50% Glycerol, 1% BSA, PBS, pH7.4.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).
Target
- Alternative Names
- PCGF4; RNF51; FLVI2/BMI1; flvi-2/bmi-1
- Gene ID
- 648
- UniProt ID
- P35226
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Monoubiquitinated (By similarity).
May be polyubiquitinated; which does not lead to proteasomal degradation.
- Protein Refseq
- NP_001190991.1; NP_005171.4
- Function
- Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:15386022, PubMed:16359901, PubMed:26151332, PubMed:16714294, PubMed:21772249, PubMed:25355358, PubMed:27827373).
The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A (PubMed:21772249, PubMed:25355358).
In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2 (PubMed:15386022, PubMed:26151332, PubMed:21772249).
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4024z | Mouse Anti-BMI1 Recombinant Antibody (clone 43H11) | WB, FC, ICC, IF, IHC | Mouse IgG1 |
| MOB-0263CT | Mouse Anti-BMI1 Recombinant Antibody (clone EML250) | WB | Mouse IgG1 |
| ZG-0973F | Mouse Anti-BMI1 Recombinant Antibody (clone 229F6) | IHC-Fr, IP, WB | Mouse IgG1 |
| ZG-0570J | Rabbit Anti-BMI1 Recombinant Antibody (clone 1F2) | ELISA, WB, IHC | Rabbit IgG |
| VS3-CJ101 | Mouse Anti-BMI1 Recombinant Antibody (VS3-CJ101) | WB, ELISA | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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