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Mouse Anti-BMI1 Recombinant Antibody (VS3-CJ22) (CAT#: VS3-CJ22)

This product is a mouse antibody that recognizes BMI1.


See more Engineered Antibody products for BMI1
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  • Gene Expression
  • Datasheet
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  • COA
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30471/1564_A5_1_selected.jpg
Figure 2 IF staining of human cell line A-431
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30472/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human adrenal gland
Figure 3 IHC staining of human adrenal gland
(Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in cortical cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/ihc_selected.jpg
Figure 4 Testis
Figure 4 Testis
(Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_6_6.jpg
Figure 5 Lymph node
Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_8_8.jpg
Figure 6 Cerebral cortex
Figure 6 Cerebral cortex
(Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_B_8_5.jpg
Figure 7 Nasopharynx
Figure 7 Nasopharynx
(Respiratory epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_B_8_2.jpg
Figure 8 Colon
Figure 8 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_7_3.jpg
Figure 9 Kidney
Figure 9 Kidney
(Collecting ducts Staining: Medium Intensity: Strong Quantity: <25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_7_5.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (HDLM-2, U-937)
Figure 10 RNA cell line category: Cell line enhanced (HDLM-2, U-937)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168283-BMI1

Specifications

  • Immunogen
  • KLH-conjugated synthetic peptide from the N-terminal region of human Bmi1
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human BMI1
  • Species Reactivity
  • Human
  • Applications
  • WB, ELISA
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Protein G affinity purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Format
  • Liquid
  • Concentration
  • 1 mg/mL (lot specific)
  • Buffer
  • 50% Glycerol, 1% BSA, PBS, pH7.4.
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).

Target

  • Alternative Names
  • PCGF4; RNF51; FLVI2/BMI1; flvi-2/bmi-1
  • Cellular Localization
  • Cytoplasm, Nucleus
  • Post Translation Modifications
  • Monoubiquitinated (By similarity).
    May be polyubiquitinated; which does not lead to proteasomal degradation.
  • Function
  • Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:15386022, PubMed:16359901, PubMed:26151332, PubMed:16714294, PubMed:21772249, PubMed:25355358, PubMed:27827373).
    The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A (PubMed:21772249, PubMed:25355358).
    In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2 (PubMed:15386022, PubMed:26151332, PubMed:21772249).

Recommended Products

Isotype Control

Secondary Antibody

Recommended Dilution Buffer

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "BMI1"

Recombinant Antibody

CAT Product Name Application Type
MOB-4024z Mouse Anti-BMI1 Recombinant Antibody (clone 43H11) WB, FC, ICC, IF, IHC Mouse IgG1
ZG-0570J Rabbit Anti-BMI1 Recombinant Antibody (clone 1F2) ELISA, WB, IHC Rabbit IgG
VS3-CJ101 Mouse Anti-BMI1 Recombinant Antibody (VS3-CJ101) WB, ELISA Mouse IgG
VS3-CJ895 Mouse Anti-BMI1 Recombinant Antibody (VS3-CJ895) WB, ICC Mouse IgG1, κ
VS3-CJ896 Rabbit Anti-BMI1 Recombinant Antibody (VS3-CJ896) WB, ICC, IF, IHC Rabbit IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0365 Hi-Affi™ Rabbit Anti-BMI1 Recombinant Antibody (clone DS365AB) WB, IHC, ICC Rabbit IgG

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-CN0449 PE-A*02:01/Human BMI1 (TLQDIVYKL) MHC Tetramer FCM, IHC
MHC-CN0450 APC-A*02:01/Human BMI1 (TLQDIVYKL) MHC Tetramer FCM, IHC
MHC-CN0451 FITC-A*02:01/Human BMI1 (TLQDIVYKL) MHC Tetramer FCM, IHC
MHC-YF337 A*02:01/Human BMI1 (CLPSPSTPV) MHC Pentamer FCM
MHC-YF338 A*02:01/Human BMI1 (TLQDIVYKL) MHC Pentamer FCM

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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