Rabbit Anti-BTK (pY551) Recombinant Antibody (clone R07-9C1)
CAT#: VS3-WK2141
This product is a rabbit recombinant antibody that is specific to phosphorylated BTK. The target of this antibody is phospho-BTK (Tyr551). The clone R07-9C1 can be used in WB analysis at a dilution of 1/500-1/1000 and IF analysis at a dilution of 1/50-1/200. The molecular weight of BTK (pTyr551) observed in WB experiments using this antibody is 76 kDa.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2028/1770_B11_1_selected.jpg
Normal Tissue
Figure 2 Colon
Enterocytes - Mucosal lymphoid cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1198/2770_A_9_3.jpg
Normal Tissue
Figure 3 High expression in lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1198/2770_A_8_8.jpg
Normal Tissue
Figure 4 Spleen
Cells in red pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in white pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1198/2770_B_8_4.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (Daudi, HEL, HMC-1, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000010671-BTK
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Tyr551 of human BTK
- Species Reactivity
- Human
- Clone
- R07-9C1
- Applications
- WB, ICC, IF
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
Target
- Alternative Names
- Bruton Tyrosine Kinase; Bruton Agammaglobulinemia Tyrosine Kinase; B-Cell Progenitor Kinase; Brutons Tyrosine Kinase; EC 2.7.10.2; AGMX1; ATK; BPK; Tyrosine-Protein Kinase BTK Isoform (Lacking Exon 13 To 17); Dominant-Negative Kinase-Deficient Brutons Tyrosine Kinase; Tyrosine-Protein Kinase BTK Isoform (Lacking Exon 14)
- Gene ID
- 695
- UniProt ID
- Q06187
- Long Name
- Bruton Tyrosine Kinase
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Related Signaling Pathways
BCR Signaling Pathway
Related Diseases
Primary Immunodeficiency
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "BTK"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2243z | Mouse Anti-BTK Recombinant Antibody (clone 21F12) | WB, FC, ICC, IF, IHC | Mouse IgG2b |
| MOB-112CQ | Mouse Anti-BTK Recombinant Antibody (clone CBLS-194) | WB, ICC | Mouse IgG1 |
| MRO-2304-CN | Rabbit Anti-BTK Recombinant Antibody (clone CBACN-597) | WB, IP | Rabbit IgG |
| ZG-0353F | Mouse Anti-Btk Recombinant Antibody (ZG-0353F) | WB, IHC, IF, ELISA | Mouse IgG |
| ZG-0354F | Mouse Anti-BTK Recombinant Antibody (ZG-0354F) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0393 | Hi-Affi™ Rabbit Anti-BTK Recombinant Antibody (clone DS393AB) | ICC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY34 | AbPlus™ Anti-BTK Magnetic Beads (CBACN-075) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY57 | Mouse Anti-NHP BTK Recombinant Antibody (clone 7F12H4) | WB, IHC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY267 | Anti-BTK Immunohistochemistry Kit | IHC | |
| VS-0525-XY854 | Anti-Mouse BTK Immunohistochemistry Kit | IHC | |
| VS-0525-XY853 | Anti-Human BTK Immunohistochemistry Kit | IHC |
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