Rabbit Anti-C3 Recombinant Antibody (clone 4D12) (CAT#: ZG-0576J)
This product is a rabbit antibody that recognizes C3.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Rabbit Anti-C3 Antibody (ZG-0576J) in WB
Western Blot
Positive WB detected in: THP-1 whole cell lysate, K562 whole cell lysate
All lanes: C3 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 188 kDa
Observed band size: 115 kDa
IHC
Figure 2 Rabbit Anti-C3 Antibody (ZG-0576J) in IHC
IHC image of ZG-0576J diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
FC
Figure 3 Rabbit Anti-C3 Antibody (ZG-0576J) in FC
Overlay histogram showing HepG2 cells stained with ZG-0576J (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.
Specifications
- Immunogen
- A synthesized peptide derived from human C3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human C3
- Species Reactivity
- Human
- Clone
- 4D12
- Applications
- ELISA, WB, IHC, FC
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Flow Cytometry.
WB:1:500-1:5000, IHC:1:50-1:200, FC:1:20-1:200
Target
Related Resources
Systemic Lupus Erythematosus
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "C3"
Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products
ScFv Antibody Products
Neutralizing Antibody Products
Blocking Antibody Products
Rabbit Monoclonal Antibody Products
Fab Antibody Products
Single Domain Antibody Products
Antibody Magnetic Beads
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-142 | Recombinant Human Anti-human C3 Antibody | ELISA, WB, IP, FuncS | IgG |
| MOR-0028-FY | Mouse Anti-C3 Recombinant Antibody (clone 5G9) | FuncS | Mouse IgG2a |
| MOR-0029-FY | Mouse Anti-C3 Recombinant Antibody (clone 3E7) | FC, FuncS, IF | Mouse IgG1 |
| FAMAB-1725CQ | Human Anti-C3 Recombinant Antibody (clone MF9) | ELISA, IHC | Human IgG1-Fc |
| FAMAB-1728CQ | Human Anti-C3 Recombinant Antibody (clone KF7) | SPR, ELISA | Human IgG1-Fc |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for ZG-0576J. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA, IP, FC, FuncS, Neut, IF, IHC
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: ELISA, FC, IP, FuncS, IF, Neut, WB
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: WB, IF, FuncS
Application: ELISA
Application: ELISA, PD, Activ, PK, Stim
For Research Use Only. Not For Clinical Use.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
