Rabbit Anti-C3 Recombinant Antibody (clone 8C3)
CAT#: ZG-0577J
This product is a rabbit antibody that recognizes C3.
Tested Data
Gene Expression
IHC
Figure 1 Rabbit Anti-C3 Antibody (ZG-0577J) in IHC
IHC image of ZG-0577J diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Normal Tissue
Figure 1 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Proximal tubules (cell body)
Staining: Medium
Intensity: Moderate
Quantity:>75%
Proximal tubules (microvilli)
Staining: Medium
Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3563/10702_A_9_5.jpg
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Specifications
- Immunogen
- A synthesized peptide derived from human C3 / C3a
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human C3
- Species Reactivity
- Human
- Clone
- 8C3
- Applications
- ELISA, IHC
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Immunohistochemistry.
IHC:1:50-1:200
Target
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Related Diseases
Systemic Lupus Erythematosus
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| NEUT-080CQ | Mouse Anti-C3 Recombinant Antibody (clone K13/16) | ELISA, IP, WB, Neut | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| PABC-558 | Recombinant Llama Anti-C3 Single Domain Antibody (PABC-558) | ELISA, Inhib | Llama VHH |
| CAT | Product Name | Application | Type |
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| VS-0525-XY880 | Anti-Mouse C3 Immunohistochemistry Kit | IHC | |
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