Mouse Anti-CALR Recombinant Antibody (VS3-FY2641) (CAT#: VS3-FY2641)
The anti-CALR antibody is a mouse recombinant monoclonal antibody that with high affinity for CALR. The calculated molecular weight of the protein is 48.1 kDa. It exhibits cross-reactivity with human, mouse and rat. This monoclonal antibody is potential for conducting research on Myelofibrosis and Thrombocythemia 1. The antibody can be utilized for detection of CALR by Western Blot, Immunohistochemistry, Flow cytometry, ELISA.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19952/ihc_selected.jpg
(Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19952/ihc_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to Endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79775/1900_C3_3_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to Endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79775/1900_C3_3_blue_red_green.jpg
Figure 3 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2242/8396_B_7_5.jpg
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2242/8396_B_7_5.jpg
Figure 4 Thyroid gland
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2242/8396_B_1_5.jpg
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2242/8396_B_1_5.jpg
Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000179218-CALR
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000179218-CALR
Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000179218-CALR
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000179218-CALR
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Calreticulin of human, mouse and rat origins
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC, FC, ELISA
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 48.1 kDa.
- Related Disease
- Myelofibrosis and Thrombocythemia 1
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/50-1/200
Flow cytometry: 1/50-1/200
ELISA: 1/1000-1/10000
Target
- Alternative Names
- Calreticulin; Sicca Syndrome Antigen A (Autoantigen Ro; Calreticulin); Endoplasmic Reticulum Resident Protein 60; Calregulin; CRP55; ERp60; HACBP; Grp60; Epididymis Secretory Sperm Binding Protein Li 99n
- Gene ID
- 811
- Sequence Similarities
- Belongs to the calreticulin family.
- Cellular Localization
- Endoplasmic reticulum lumen
Cytoplasm, cytosol
Secreted, extracellular space, extracellular matrix
Cell surface
Sarcoplasmic reticulum lumen
Cytoplasmic vesicle, secretory vesicle, Cortical granule
Cytolytic granule
- Post Translation Modifications
- Glycosylation at Asn344
Ubiquitination at Lys24, Lys48, Lys55, and Lys159
Modification sites at PhosphoSitePlus
Glycosylation from Gly
Glycosylation from GlyGen 3 sites, 10 N-linked glycans (1 site), 2 O-linked glycans (2 sites)
- Protein Refseq
- NP_004334.1
- Function
- Involved in maternal gene expression regulation.
May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity).
Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy (By similarity).
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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