Mouse Anti-CALR Recombinant Antibody (VS3-FY2641)
CAT#: VS3-FY2641
The anti-CALR antibody is a mouse recombinant monoclonal antibody that with high affinity for CALR. The calculated molecular weight of the protein is 48.1 kDa. It exhibits cross-reactivity with human, mouse and rat. This monoclonal antibody is potential for conducting research on Myelofibrosis and Thrombocythemia 1. The antibody can be utilized for detection of CALR by Western Blot, Immunohistochemistry, Flow cytometry, ELISA.
Gene Expression
Subcellular Location
Figure 1 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19952/ihc_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to Endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79775/1900_C3_3_blue_red_green.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2242/8396_B_7_5.jpg
Normal Tissue
Figure 4 Thyroid gland
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2242/8396_B_1_5.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000179218-CALR
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Calreticulin of human, mouse and rat origins
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC, FC, ELISA
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 48.1 kDa.
- Related Disease
- Myelofibrosis and Thrombocythemia 1
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/50-1/200
Flow cytometry: 1/50-1/200
ELISA: 1/1000-1/10000
Target
- Alternative Names
- Calreticulin; Sicca Syndrome Antigen A (Autoantigen Ro; Calreticulin); Endoplasmic Reticulum Resident Protein 60; Calregulin; CRP55; ERp60; HACBP; Grp60; Epididymis Secretory Sperm Binding Protein Li 99n
- Gene ID
- 811
- Sequence Similarities
- Belongs to the calreticulin family.
- Cellular Localization
- Endoplasmic reticulum lumen
Cytoplasm, cytosol
Secreted, extracellular space, extracellular matrix
Cell surface
Sarcoplasmic reticulum lumen
Cytoplasmic vesicle, secretory vesicle, Cortical granule
Cytolytic granule
- Post Translation Modifications
- Glycosylation at Asn344
Ubiquitination at Lys24, Lys48, Lys55, and Lys159
Modification sites at PhosphoSitePlus
Glycosylation from Gly
Glycosylation from GlyGen 3 sites, 10 N-linked glycans (1 site), 2 O-linked glycans (2 sites)
- Protein Refseq
- NP_004334.1
- Function
- Involved in maternal gene expression regulation.
May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity).
Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CALR"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0287CT | Mouse Anti-CALR Recombinant Antibody (clone EML269) | IHC-P, WB | Mouse IgG1 |
| HPAB-0446-YJ | Mouse Anti-CALR Recombinant Antibody (clone FMMU-CRT-10) | FC, WB, CLEIA, ELISA, IHC | Mouse IgG1, κ |
| HPAB-0447-YJ | Human Anti-CALR Recombinant Antibody (clone chFMMU-CRT-10) | ELISA, WB, IHC, FC | Chimeric (mouse/human) IgG1, κ |
| VS3-CJ840 | Rabbit Anti-CALR Recombinant Antibody (VS3-CJ840) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS-0923-FY25 | Recombinant Mouse Anti-CALR Antibody (VS-0923-FY25) | ELISA, WB | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0076MZ | Chicken Anti-Calreticulin Polyclonal IgY | IHC | Chicken antibody |
| BRD-0772MZ | Chicken Anti-Calreticulin Polyclonal IgY | Blocking Assay (BLOCK), IHC (Paraffin) (IHC (P)), ICC (ICC), Flow Cytometry (Flow), IHC (IHC) | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1043 | PE-A*02:01/Human CALR (MLLSVPLLLG) MHC Tetramer | FCM | |
| MHC-LC1044 | APC-A*02:01/Human CALR (MLLSVPLLLG) MHC Tetramer | FCM | |
| MHC-LC1045 | BV421-A*02:01/Human CALR (MLLSVPLLLG) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0443 | Hi-Affi™ Rabbit Anti-CALR Recombinant Antibody (clone DS443AB) | WB, IHC, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0271-YC-S(P) | Mouse Anti-CALR Recombinant Antibody (clone 8B2-H6-10.7); scFv Fragment | FC, WB | Mouse scFv |
| HPAB-0446-YJ-S(P) | Mouse Anti-CALR Recombinant Antibody (clone FMMU-CRT-10); scFv Fragment | ELISA, WB, IHC, FC | Mouse scFv |
| HPAB-0447-YJ-S(P) | Mouse Anti-CALR Recombinant Antibody (clone chFMMU-CRT-10); scFv Fragment | ELISA, WB, IHC, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0271-YC-F(E) | Mouse Anti-CALR Recombinant Antibody (clone 8B2-H6-10.7); Fab Fragment | FC, WB | Mouse Fab |
| HPAB-0446-YJ-F(E) | Mouse Anti-CALR Recombinant Antibody (clone FMMU-CRT-10); Fab Fragment | ELISA, WB, IHC, FC | Mouse Fab |
| HPAB-0447-YJ-F(E) | Human Anti-CALR Recombinant Antibody (clone chFMMU-CRT-10); Fab Fragment | ELISA, WB, IHC, FC | Chimeric (mouse/human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC841 | AbPlus™ Anti-CALR Magnetic Beads (VS-0724-YC841) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY61 | Mouse Anti-NHP CALR Recombinant Antibody (clone FMC 75) | WB, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY297 | Anti-CALR Immunohistochemistry Kit | IHC | |
| VS-0525-XY946 | Anti-Mouse CALR Immunohistochemistry Kit | IHC | |
| VS-0525-XY947 | Anti-Human CALR Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC114 | CytoStream™ Rabbit Anti-CALR Recombinant Antibody (VS13-YC114) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
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