Mouse Anti-CCR3 Recombinant Antibody (clone 7B11) (CAT#: FAMAB-0308-CN)

Figure 1 Involvement of CCR3 in MAPkinase activation by eotaxin.

Eosinophils were preincubated with blocking monoclonal anti-CCR3 antibodies (7B11) or isotypespecific control antibodies for 30 minutes and stimulated with eotaxin (10-8 mol/L) for 1 minute. Cytosolic extracts underwent Western blotting with antibodies against dual threonine-tyrosine–phosphorylated ERK2 (a-PT/PY-ERK1/2) (A) or dual threoninetyrosine–phosphorylated p38 (a-PT/PY-p38) (B). 7B11 blocked the phosphorylation of both ERK2 and p38.

Kampen, G. T., Stafford, S., Adachi, T., Jinquan, T., Quan, S., Grant, J. A., ... & Alam, R. (2000). Eotaxin induces degranulation and chemotaxis of eosinophils through the activation of ERK2 and p38 mitogen-activated protein kinases: Presented in part at the annual meetings of the American Academy of Allergy, Asthma, and Immunology in Washington, DC, March 13-18, 1998, and in Orlando, FL, February 26-March 3, 1999. Blood, 95(6), 1911-1917.

Figure 2 CCR3 functional assays.

Western blot analysis demonstrating the kinetics of phosphorylation of the p42 mitogen-activated protein kinase in eotaxin-stimulated PBECs. PBECs were preincubated with media alone or pertussis toxin (1 μg/ml, 1 h) and stimulated with eotaxin (100 ng/ml) for up to 1 min. Similar results were noted when lysates were analyzed by immunoblot for eotaxin-induced tyrosine phosphorylation with and without preincubation with the CCR3-blocking mAb 7B11. (These are representative blots of n = 3 for primary epithelial cells and n = 3 for 16-HBE cells.) To ensure equal loading in each lane, we load each lane with 10 μg of total protein (measured by the bicinchoninic acid assay) and run a parallel immunoblot probed with p42/p44 anti-ERK Ab.

Stellato, C., Brummet, M. E., Plitt, J. R., Shahabuddin, S., Baroody, F. M., Liu, M. C., ... & Beck, L. A. (2001). Cutting edge: expression of the CC chemokine receptor CCR3 in human airway epithelial cells. The Journal of Immunology, 166(3), 1457-1461.