Mouse Anti-CCR3 Recombinant Antibody (clone 7B11)
CAT#: FAMAB-0308-CN
The antibody 7B11 is specific for CCR3 and has the properties of a true receptor antagonist. 7B11 blockes binding of various radiolabeled chemokines to either CCR3 transfectants, or eosinophils. Pretreatment of eosinophils with this mAb blockes chemotaxis and calcium flux induced by all CCR3 ligands.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69514/1943_C10_3_selected.jpg
Normal Tissue
Figure 2 Esophagus
Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3795/10573_B_6_1.jpg
Normal Tissue
Figure 3 Liver
Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3795/10573_A_7_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3795/10573_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3795/10573_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3795/10573_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enriched (RPMI-8226)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000183625-CCR3
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Specifications
- Immunogen
- L1.2 transfectants expressing high levels of CCR3
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CCR3
- Species Reactivity
- Human
- Clone
- 7B11
- Applications
- FC, IF, WB
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Flow Cytometry, Immunofluorescence and Western Blot.
Target
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Exceptional Specificity
In my flow cytometry tests, the Mouse Anti-CCR3 Recombinant Antibody (clone 7B11) demonstrated exceptional specificity. It stained CCR3-positive cells only, avoiding background interference.
Compatibility with Western Blot
I found the Western Blot assay results to be really impressive. The CCR3 protein was clearly identified by the antibody at the anticipated molecular weight.
Useful in Studying Allergic Inflammation
We have used this antibody in asthmatic mouse models, and its accurate identification of CCR3 has improved our knowledge of the mechanisms underlying allergic inflammation.
Q&As
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Can this antibody be used in Western Blotting?
A: Indeed, CCR3 protein expression in cell lysates can be found using Western Blotting and the Mouse Anti-CCR3 Recombinant Antibody (clone 7B11).
-
What are the potential research applications of this antibody?
A: Applications of research include the study of Th2 immune responses, asthma etiology, allergic inflammation, and eosinophil biology.
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Is the antibody suitable for use in flow cytometry?
A: Without a doubt, flow cytometry is a suitable method for examining CCR3 expression on the surface of different cell types.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| MOB-1624z | Mouse Anti-CCR3 Recombinant Antibody (clone 27C5) | WB, ELISA, FC | Mouse IgG1 |
| MOB-0369CT | Mouse Anti-CCR3 Recombinant Antibody (clone 6F9) | FC, IF, IHC-Fr | Mouse IgG2a |
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| CAT | Product Name | Application | Type |
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| NEUT-266CQ | Rat Anti-CCR3 Recombinant Antibody (clone CBL302) | FC, IHC, CyTOF, Neut | Rat IgG2a |
| CAT | Product Name | Application | Type |
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| MOR-0521 | Hi-Affi™ Rabbit Anti-CCR3 Recombinant Antibody (clone DS521AB) | WB, IHC-P, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0308-CN-S(P) | Mouse Anti-CCR3 Recombinant Antibody (clone 7B11); scFv Fragment | FC, IF | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0308-CN-F(E) | Mouse Anti-CCR3 Recombinant Antibody (clone 7B11); Fab Fragment | FC, IF | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1047 | AbPlus™ Anti-CCR3 Magnetic Beads (VS-0724-YC1047) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
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| VS-0425-YC731 | Recombinant Anti-CCR3 Vesicular Antibody, EV Displayed (VS-0425-YC731) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
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