Mouse Anti-CD3 Recombinant Antibody (clone 898H2-6-15) (CAT#: FAMAB-0043CQ)

This product is a recombinant mouse antibody clone 898H2-6-15, which specifically binds to CD3.


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ELISA

Figure 1 ELISA binding reactivity of dilutions of 898H2-6-15 mAb to porcine CD3Ɛγ single-chain fusion protein coated plates.

Figure 1 ELISA binding reactivity of dilutions of 898H2-6-15 mAb to porcine CD3Ɛγ single-chain fusion protein coated plates.

Porcine CD3Ɛγ single-chain fusion protein binding reactivity spans almost 2 log range of 898H2-6-15 mAb dilutions.

Peraino, J. S., Hermanrud, C. E., Springett, L., Zhang, H., Li, G., Srinivasan, S., ... & Wang, Z. (2012). Expression and characterization of recombinant soluble porcine CD3 ectodomain molecules: mapping the epitope of an anti-porcine CD3 monoclonal antibody 898H2-6-15. Cellular immunology, 276(1-2), 162-167.

ELISA

Figure 2 Competition ELISA. 898H2-6-15 binding to porcine CD3ec single-chain fusion protein coated plates can be competitively inhibited by addition of soluble porcine CD3Ɛγ single-chain fusion protein.

Figure 2 Competition ELISA. 898H2-6-15 binding to porcine CD3ec single-chain fusion protein coated plates can be competitively inhibited by addition of soluble porcine CD3Ɛγ single-chain fusion protein.

898H2-6-15 mAb is pre-incubated with the porcine CD3ec single-chain fusion protein at the indicated concentrations and then added to the plate. Data representative of three experiments.

Peraino, J. S., Hermanrud, C. E., Springett, L., Zhang, H., Li, G., Srinivasan, S., ... & Wang, Z. (2012). Expression and characterization of recombinant soluble porcine CD3 ectodomain molecules: mapping the epitope of an anti-porcine CD3 monoclonal antibody 898H2-6-15. Cellular immunology, 276(1-2), 162-167.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG2a, κ
  • Specificity
  • Porcine CD3
  • Species Reactivity
  • Porcine
  • Clone
  • 898H2-6-15
  • Applications
  • ELISA, FuncS
  • Related Disease
  • Autoimmune disease

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Enzyme-linked Immunosorbent Assay.

Target

  • Alternative Names
  • CD3; CD3 T-Cell Co-receptor;CD3D; CD3E; CD3G

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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Chimeric Antibody

CAT Product Name Application Type
TAB-0417CL-S(P) Chimeric Anti-Human CD3 Recombinant Antibody scFv Fragment ELISA, FC, FuncS Chimeric (mouse/human)
TAB-071LC-S(P) Anti-Human CD3 Recombinant Antibody scFv Fragment ELISA Chimeric antibody (mouse/human)
TAB-072LC-S(P) Chimeric Anti-Human CD3 Recombinant Antibody scFv Fragment ELISA Chimeric antibody (mouse/human)
TAB-071LC-F(E) Anti-Human CD3 Recombinant Antibody Fab Fragment ELISA Chimeric antibody (mouse/human)
TAB-072LC-F(E) Chimeric Anti-Human CD3 Recombinant Antibody Fab Fragment ELISA Chimeric antibody (mouse/human)

Blocking Antibody

CAT Product Name Application Type
NEUT-339CQ Mouse Anti-CD3 Recombinant Antibody (clone SPV-T3b) FC, Block, IHC-Fr, IF, WB Mouse IgG2a
NEUT-340CQ Mouse Anti-CD3 Recombinant Antibody (clone UCHT1) FC, IHC-Fr, IHC-P, ICC, IP, WB, Activ, Block Mouse IgG1, κ

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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