Mouse Anti-CD3 Recombinant Antibody (clone 898H2-6-15) (CAT#: FAMAB-0043CQ)

This product is a recombinant mouse antibody clone 898H2-6-15, which specifically binds to CD3.

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ELISA

Figure 1 ELISA binding reactivity of dilutions of 898H2-6-15 mAb to porcine CD3Ɛγ single-chain fusion protein coated plates.

Porcine CD3Ɛγ single-chain fusion protein binding reactivity spans almost 2 log range of 898H2-6-15 mAb dilutions.

Peraino, J. S., Hermanrud, C. E., Springett, L., Zhang, H., Li, G., Srinivasan, S., ... & Wang, Z. (2012). Expression and characterization of recombinant soluble porcine CD3 ectodomain molecules: mapping the epitope of an anti-porcine CD3 monoclonal antibody 898H2-6-15. Cellular immunology, 276(1-2), 162-167.

ELISA

Figure 2 Competition ELISA. 898H2-6-15 binding to porcine CD3ec single-chain fusion protein coated plates can be competitively inhibited by addition of soluble porcine CD3Ɛγ single-chain fusion protein.

898H2-6-15 mAb is pre-incubated with the porcine CD3ec single-chain fusion protein at the indicated concentrations and then added to the plate. Data representative of three experiments.

Peraino, J. S., Hermanrud, C. E., Springett, L., Zhang, H., Li, G., Srinivasan, S., ... & Wang, Z. (2012). Expression and characterization of recombinant soluble porcine CD3 ectodomain molecules: mapping the epitope of an anti-porcine CD3 monoclonal antibody 898H2-6-15. Cellular immunology, 276(1-2), 162-167.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG2a, κ
  • Specificity
  • Porcine CD3
  • Species Reactivity
  • Porcine
  • Clone
  • 898H2-6-15
  • Applications
  • ELISA, FuncS
  • Related Disease
  • Autoimmune disease

Applications

  • Application Notes
  • This antibody has been tested for use in Enzyme-linked Immunosorbent Assay.

Target

  • Alternative Names
  • CD3; CD3 T-Cell Co-receptor;CD3D; CD3E; CD3G

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Blocking Antibody

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scFv Fragment Antibody

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Single-domain Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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