Mouse Anti-CD3 Recombinant Antibody (clone FN18)
CAT#: FAMAB-0044CQ
This product is a recombinant mouse antibody clone FN18, which specifically binds to CD3.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG, κ
- Specificity
- Rhesus monkey CD3
- Species Reactivity
- Rhesus monkey
- Clone
- FN18
- Applications
- ELISA, WB, FC, FuncS
- Related Disease
- Autoimmune disease
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Enzyme-linked Immunosorbent Assay
The CD3 ectodomain or their homo- and heterodimers at 10 μg/ml in PBS were adsorbed to Costar 96-well EIA/Radioimmunoassay flat bottom plates (Corning Inc., NY, Cat# 3590) overnight at 4 centigrade or 2 h at room temperature and then washed three times with PBS containing 0.1% Tween 20. Wells were then incubated for 1 h at room temperature with 3% gelatin in PBS to prevent non-specific binding to the plastic. The wells were then washed as earlier, and incubated for 1 h with FN18 or SP34 mAb (10μg/ml) in PBS containing 0.1% Tween 20. Wells were washed and incubated for another hour with 100 μl goat anti-mouse IgG1 mAb conjugated with alkaline phosphatase(1:1000) or alkaline phosphatase conjugated Protein A for SP34. Wells were washed and alkaline phosphatase substrate (Pierce) was added following the manufacturer's directions. After 30 min, color development was stopped with NaOH and the optical density (OD) was measured with an Enzyme-linked Immunosorbent Assay reader using a 405 nm filter. Each sample was performed in triplicate. Soluble competition Enzyme-linked Immunosorbent Assay was performed by binding εγ heterodimer to the plastic as above and blocking and washing as above. FN18 antibody at 2 μg/ml was mixed with various concentrations competitor CD3 chains. Detection of FN18 binding to the solid phase was done as above. Solid phase competition Enzyme-linked Immunosorbent Assay was performed by mixing various ratios of CD3 chain competitors with εγ heterodimer and attaching these mixtures to the plates. FN18 binding to the solid phase was detected as before. UCHT1 served as an IgG1 isotype control for FN18.
Target
- Alternative Names
- CD3; CD3 T-Cell Co-receptor;CD3D; CD3E; CD3G
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High purity and quality
This antibody's purification quality is excellent. Because of the high purity, our assays perform better and yield consistent, repeatable findings each time.
Excellent technical support
Although there were some early problems, the technical assistance was quick and effective. They offered insightful commentary and helpful corrections that enabled us to get the best outcomes. Excellent client support.
Useful in knockout validation
This antibody was exactly what we needed to validate our knockdown research. The antibody's dependability was validated by the obvious specificity and the absence of signal in knockout samples.
Q&As
-
What concentration is the antibody supplied at?
A: The standard concentration of the antibody is 1 mg/mL, however this can change. See the product datasheet for further information.
-
Are there any known issues with cross-reactivity?
A: The antibody has a low degree of cross-reactivity by design. However, if utilizing the antibody in a species for which it has not been formally validated, always conduct control tests to evaluate potential cross-reactivity with non-target proteins.
-
Can I get technical support if I have issues with the antibody?
A: Yes, there is access to extensive technical help. For individualized support, get in touch with the manufacturer's customer care and provide them with comprehensive details about your experiment and any problems you are having.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# FAMAB-0044CQ, RRID: AB_3111304)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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