Mouse Anti-CD3 Recombinant Antibody (clone FN18) (CAT#: FAMAB-0044CQ)

This product is a recombinant mouse antibody clone FN18, which specifically binds to CD3.


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ELISA

Figure 1 The FN18 reactivity by ELISA of the chromatographed CD3 chain fractions, attached to the plastic plate surface, from the ε, γ and δ chain refoldings are shown.

Figure 1 The FN18 reactivity by ELISA of the chromatographed CD3 chain fractions, attached to the plastic plate surface, from the ε, γ and δ chain refoldings are shown.

ELISA OD values are on the vertical axis, elution fractions on the horizontal axis and the individual and joint chain refoldings
are listed on the angular axis. The peak reactivity corresponds to the peak of εγ chain elution. Detection of FN18 binding to the bottom layer is detected by anti-mouse IgG1 mAb conjugated with alkaline phosphatase.

Wang, Z., & Neville Jr, D. M. (2004). Expression and characterization of recombinant soluble monkey CD3 molecules: mapping the FN18 polymorphic epitope. Molecular immunology, 40(16), 1179-1188.

ELISA

Figure 2 The εγ chain reactivity by ELISA spans a 2 log range of FN18 dilutions. εγ chains are attached to the plastic plate surface.

Figure 2 The εγ chain reactivity by ELISA spans a 2 log range of FN18 dilutions. εγ chains are attached to the plastic plate surface.

Wang, Z., & Neville Jr, D. M. (2004). Expression and characterization of recombinant soluble monkey CD3 molecules: mapping the FN18 polymorphic epitope. Molecular immunology, 40(16), 1179-1188.

ELISA

Figure 3 Competition ELISA.

Figure 3 Competition ELISA.

εγ chains are attached to the plastic plate. FN18 is preincubated with the various CD3 chain elution fractions at the indicated concentrations and then added to plate. After washing, detection of FN18 binding to the plate.

Wang, Z., & Neville Jr, D. M. (2004). Expression and characterization of recombinant soluble monkey CD3 molecules: mapping the FN18 polymorphic epitope. Molecular immunology, 40(16), 1179-1188.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG, κ
  • Specificity
  • Rhesus monkey CD3
  • Species Reactivity
  • Rhesus monkey
  • Clone
  • FN18
  • Applications
  • ELISA, WB, FC, FuncS
  • Related Disease
  • Autoimmune disease

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Enzyme-linked Immunosorbent Assay
    The CD3 ectodomain or their homo- and heterodimers at 10 μg/ml in PBS were adsorbed to Costar 96-well EIA/Radioimmunoassay flat bottom plates (Corning Inc., NY, Cat# 3590) overnight at 4 centigrade or 2 h at room temperature and then washed three times with PBS containing 0.1% Tween 20. Wells were then incubated for 1 h at room temperature with 3% gelatin in PBS to prevent non-specific binding to the plastic. The wells were then washed as earlier, and incubated for 1 h with FN18 or SP34 mAb (10μg/ml) in PBS containing 0.1% Tween 20. Wells were washed and incubated for another hour with 100 μl goat anti-mouse IgG1 mAb conjugated with alkaline phosphatase(1:1000) or alkaline phosphatase conjugated Protein A for SP34. Wells were washed and alkaline phosphatase substrate (Pierce) was added following the manufacturer's directions. After 30 min, color development was stopped with NaOH and the optical density (OD) was measured with an Enzyme-linked Immunosorbent Assay reader using a 405 nm filter. Each sample was performed in triplicate. Soluble competition Enzyme-linked Immunosorbent Assay was performed by binding εγ heterodimer to the plastic as above and blocking and washing as above. FN18 antibody at 2 μg/ml was mixed with various concentrations competitor CD3 chains. Detection of FN18 binding to the solid phase was done as above. Solid phase competition Enzyme-linked Immunosorbent Assay was performed by mixing various ratios of CD3 chain competitors with εγ heterodimer and attaching these mixtures to the plates. FN18 binding to the solid phase was detected as before. UCHT1 served as an IgG1 isotype control for FN18.

Target

  • Alternative Names
  • CD3; CD3 T-Cell Co-receptor;CD3D; CD3E; CD3G

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Chimeric Antibody

CAT Product Name Application Type
TAB-071LC Anti-Human CD3 Recombinant Antibody ELISA Chimeric antibody (mouse/human)
TAB-072LC Chimeric Anti-Human CD3 Recombinant Antibody ELISA Chimeric antibody (mouse/human)
TAB-071LC-S(P) Anti-Human CD3 Recombinant Antibody scFv Fragment ELISA Chimeric antibody (mouse/human)
TAB-072LC-S(P) Chimeric Anti-Human CD3 Recombinant Antibody scFv Fragment ELISA Chimeric antibody (mouse/human)
TAB-071LC-F(E) Anti-Human CD3 Recombinant Antibody Fab Fragment ELISA Chimeric antibody (mouse/human)

Humanized Antibody

Blocking Antibody

CAT Product Name Application Type
NEUT-339CQ Mouse Anti-CD3 Recombinant Antibody (clone SPV-T3b) FC, Block, IHC-Fr, IF, WB Mouse IgG2a
NEUT-340CQ Mouse Anti-CD3 Recombinant Antibody (clone UCHT1) FC, IHC-Fr, IHC-P, ICC, IP, WB, Activ, Block Mouse IgG1, κ

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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