Mouse Anti-CD47 Recombinant Antibody (clone 2D14)
CAT#: VS11-QX36
This product is a recombinant mouse antibody that binds the extracellular domain of the human CD47. This antibody can be used for the following applications: ELISA, FC. This antibody is capable of detecting CD47 expressed on the cell surface in a flow cytometry.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16055/204_F3_1_selected.jpg
Subcellular Location
Figure 2 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows strong cytoplasmic and membranous positivity in urothelial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16055/205_F3_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/124851_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/124851_A_8_3.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/124851_A_4_6.jpg
Normal Tissue
Figure 7 Prostate
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/124851_A_2_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000196776-CD47
❮
❯
Specifications
- Immunogen
- Recombinant human CD47 extracellular domain
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human CD47
- Species Reactivity
- Human
- Clone
- 2D14
- Applications
- ELISA, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- 1 mg/mL (Please refer to the vial label for the specific concentration.)
- Buffer
- 0.2 μm filtered solution in PBS
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipped at 4°C.
Target
- Alternative Names
- CD47 Molecule variant GLT_V6F; GLTSFFIAI
- Gene ID
- 961
- UniProt ID
- Q08722
- Long Name
- Leukocyte surface antigen CD47
- Cellular Localization
- Vesicles, Plasma membrane
- Function
- Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
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- Product Name
Secondary Antibody
- CAT
- Product Name
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| TAB-279LC-S(P) | Human Anti-CD47 Recombinant Antibody; scFv Fragment (TAB-279LC-S(P)) | ELISA | Human scFv |
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|---|---|---|---|
| TAB-280LC | Human Anti-CD47 Recombinant Antibody (TAB-280LC) | ELISA | Humanized antibody |
| TAB-281LC | Human Anti-CD47 Recombinant Antibody (TAB-281LC) | ELISA | Humanized antibody |
| TAB-282LC | Human Anti-CD47 Recombinant Antibody (TAB-282LC) | ELISA | Humanized antibody |
| TAB-283LC | Human Anti-CD47 Recombinant Antibody (TAB-283LC) | ELISA | Humanized antibody |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| HPAB-0093-CN-F(E) | Human Anti-CD47 Recombinant Antibody; Fab Fragment (HPAB-0093-CN-F(E)) | ELISA, FC | Chimeric (mouse/human) Fab |
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| HPAB-N0332-YC-F(E) | Mouse Anti-CD47 Recombinant Antibody; Fab Fragment (HPAB-N0332-YC-F(E)) | ELISA, FC, Block | Mouse Fab |
| HPAB-N0333-YC-F(E) | Mouse Anti-CD47 Recombinant Antibody; Fab Fragment (HPAB-N0333-YC-F(E)) | ELISA, FC, Block | Mouse Fab |
| HPAB-N0334-YC-F(E) | Human Anti-CD47 Recombinant Antibody; Fab Fragment (HPAB-N0334-YC-F(E)) | ELISA, Block | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1082LY-S(P) | Human Anti-CD47 Recombinant Antibody; scFv Fragment (HPAB-1082LY-S(P)) | ELISA | Human scfv |
| HPAB-1083LY-S(P) | Human Anti-CD47 Recombinant Antibody; scFv Fragment (HPAB-1083LY-S(P)) | ELISA | Human scfv |
| HPAB-1084LY-S(P) | Human Anti-CD47 Recombinant Antibody; scFv Fragment (HPAB-1084LY-S(P)) | ELISA | Human scfv |
| HPAB-1085LY-S(P) | Human Anti-CD47 Recombinant Antibody; scFv Fragment (HPAB-1085LY-S(P)) | ELISA | Human scfv |
| HPAB-1086LY-S(P) | Human Anti-CD47 Recombinant Antibody; scFv Fragment (HPAB-1086LY-S(P)) | ELISA | Human scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0310-CN | Mouse Anti-CD47 Recombinant Antibody (clone 1A9) | IF, IHC, FC | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP496-YC | Recombinant Alpaca Anti-CD47 Single Domain Antibody (HPAB-AP496-YC) | FC, BL, FuncS | Alpaca VHH |
| HPAB-AP497-YC | Recombinant Alpaca Anti-CD47 Single Domain Antibody (HPAB-AP497-YC) | FC | Alpaca VHH |
| NBD-0016-YJ-VHH | Camelid Anti-CD47 Recombinant Single Domain Antibody (NBD-0016-YJ-VHH) | ELISA, SPR | Camelid VHH |
| NBD-0017-YJ-VHH | Camelid Anti-CD47 Recombinant Single Domain Antibody (NBD-0017-YJ-VHH) | ELISA, SPR | Camelid VHH |
| NBD-0018-YJ-VHH | Camelid Anti-CD47 Recombinant Single Domain Antibody (NBD-0018-YJ-VHH) | ELISA, SPR | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY99 | Mouse Anti-NHP CD47 Recombinant Antibody (clone MEM-122) | FC | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY55 | CytoStream™ Mouse Anti-CD47 Recombinant Antibody (clone B6H12) | FC | Mouse IgG1, kappa |
| VS-0225-XY56 | CytoStream™ Mouse Anti-CD47 Recombinant Antibody (clone CC2C6) | FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC635 | Recombinant Anti-CD47 Vesicular Antibody, EV Displayed (VS-0425-YC635) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
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| VS-0525-XY1191 | Anti-CD47 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1192 | Anti-Human CD47 Immunohistochemistry Kit | IHC |
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