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  • Mouse Anti-CD47 Recombinant Antibody (clone 2D14)

Mouse Anti-CD47 Recombinant Antibody (clone 2D14) (CAT#: VS11-QX36)

This product is a recombinant mouse antibody that binds the extracellular domain of the human CD47. This antibody can be used for the following applications: ELISA, FC. This antibody is capable of detecting CD47 expressed on the cell surface in a flow cytometry.

Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
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  • Fc Engineering:
  • Modalities:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location
Figure 1 IF staining of human cell line A-431
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16055/204_F3_1_selected.jpg
Figure 2 IHC staining of human urinary bladder
Figure 2 IHC staining of human urinary bladder
(Immunohistochemical staining of human urinary bladder shows strong cytoplasmic and membranous positivity in urothelial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/ihc_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16055/205_F3_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Figure 4 Cerebral cortex
(Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/124851_B_8_5.jpg
Figure 5 Colon
Figure 5 Colon
(Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/124851_A_8_3.jpg
Figure 6 Testis
Figure 6 Testis
(Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/124851_A_4_6.jpg
Figure 7 Prostate
Figure 7 Prostate
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44659/124851_A_2_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000196776-CD47
Figure 8 RNA cell line category: Low cell line specificity
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000196776-CD47

Specifications

  • Immunogen
  • Recombinant human CD47 extracellular domain
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • Mouse IgG1, κ
  • Specificity
  • Human CD47
  • Species Reactivity
  • Human
  • Clone
  • 2D14
  • Applications
  • ELISA, FC
  • Conjugate
  • Unconjugated

Product Property

  • Clonality
  • Monoclonal
  • Purification
  • Protein A purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • 1 mg/mL (Please refer to the vial label for the specific concentration.)
  • Buffer
  • 0.2 μm filtered solution in PBS
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
  • Shipping
  • Shipped at 4°C.

Target

  • Alternative Names
  • CD47 Molecule variant GLT_V6F; GLTSFFIAI
  • Long Name
  • Leukocyte surface antigen CD47
  • Cellular Localization
  • Vesicles, Plasma membrane
  • Function
  • Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.

Recommended Products

Isotype Control

Recommended Dilution Buffer

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "CD47"

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Please select product type
Human Antibody Products Humanized Antibody Products Blocking Antibody Products Neutralizing Antibody Products Rabbit Monoclonal Antibody Products Single Domain Antibody Products ScFv Antibody Products IgG Antibody Products Fab Antibody Products IgM Antibody Products Non-Human Primate (NHP) Antibody Products Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
CAT Product Name Application Type
TAB-278LC Human Anti-CD47 Recombinant Antibody (TAB-278LC) Block, FuncS Human IgG, κ
TAB-279LC Human Anti-CD47 Recombinant Antibody (TAB-279LC) Block, FuncS Human IgG, κ
TAB-278LC-S(P) Human Anti-CD47 Recombinant Antibody; scFv Fragment (TAB-278LC-S(P)) ELISA Human scFv
TAB-279LC-S(P) Human Anti-CD47 Recombinant Antibody; scFv Fragment (TAB-279LC-S(P)) ELISA Human scFv
TAB-278LC-F(E) Human Anti-CD47 Recombinant Antibody; Fab Fragment (TAB-278LC-F(E)) ELISA Human Fab

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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