Mouse Anti-CD48 Recombinant Antibody (clone 6F9H2) (CAT#: ZG-0260U)

Figure 1 Mouse Anti-CD48 Antibody (ZG-0260U) in FC

Positive WB detected in: Jurkat whole cell lysate, Raji whole cell lysate, Daudi whole cell lysate, Mouse liver tissue. All lanes: CD48 antibody at 1:4000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 28, 20KDa
Observed band size: 43 KDa
Exposure time:5min

Figure 2 Mouse Anti-CD48 Antibody (ZG-0260U) in IF

Immunofluorescence staining of JK cells with ZG-0260U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Mouse IgG (H+L).

Figure 3 Mouse Anti-CD48 Antibody (ZG-0260U) in FC

Overlay Peak curve showing JK cells stained with ZG-0260U (red line) at 1:200. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.