Mouse Anti-CD74 Recombinant Antibody (VS3-CJ338)
CAT#: VS3-CJ338
This product is a mouse antibody that recognizes CD74.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human spleen
Immunohistochemical staining of human spleen shows strong granular cytoplasmic positivity.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10592/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79994/170659_A_6_8_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/203_F11_1_blue_red_green.jpg
Normal Tissue
Figure 5 Colon
Mucosal lymphoid cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_8_5.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_8_8.jpg
Normal Tissue
Figure 8 Tonsil
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_6_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (CAPAN-2, Daudi, HDLM-2, REH, RPMI-8226, U-266/70, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000019582-CD74
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Specifications
- Immunogen
- KLH-conjugated synthetic peptide encompassing a sequence within human CD74
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CD74
- Species Reactivity
- Human
- Applications
- IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Immunogen affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.2% BSA, PBS.
- Preservative
- 0.01% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Immunohistochemistry (1:100-1:300).
Target
- Alternative Names
- II; p33; CLIP; DHLAG; HLADG; Ia-GAMMA
- Gene ID
- 972
- UniProt ID
- P04233
- Cellular Localization
- Cell membrane, Endoplasmic reticulum, Endosome, Golgi apparatus, Lysosome, Membrane
- Post Translation Modifications
- N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
- Protein Refseq
- NP_001020329.1; NP_001020330.1; NP_004346.1
- Function
- Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
Class-II-associated invariant chain peptide
Binds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome.
Isoform p41:
Stabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs). Has antiviral activity by stymieing the endosomal entry of Ebola virus and coronaviruses, including SARS-CoV-2 (PubMed:32855215).
Disrupts cathepsin-mediated Ebola virus glycoprotein processing, which prevents viral fusion and entry. This antiviral activity is specific to p41 isoform (PubMed:32855215).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0004CL-VHH | Anti-Human CD74 VHH Single Domain Antibody (TAB-0004CL-VHH) | ELISA, FC, Internalization, Inhib, FuncS | VHH |
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| TAB-0007CL-VHH | Anti-Human CD74 VHH Single Domain Antibody (TAB-0007CL-VHH) | ELISA, FC, Internalization, Inhib, FuncS | VHH |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0569CL | Human Anti-CD74 Recombinant Antibody (TAB-0569CL) | IHC, WB | Human IgG |
| TAB-0570CL | Human Anti-CD74 Recombinant Antibody (TAB-0570CL) | ELISA, FC | Human IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-408CQ | Mouse Anti-CD74 Recombinant Antibody (clone LN2) | FC, IHC-Fr, IHC-P, ICC, IP, WB, Block | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0579 | Hi-Affi™ Rabbit Anti-CD74 Recombinant Antibody (clone DS579AB) | ICC, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0388-FY-S(P) | Human Anti-CD74 Recombinant Antibody; scFv Fragment (HPAB-0388-FY-S(P)) | FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0388-FY-F(E) | Human Anti-CD74 Recombinant Antibody; Fab Fragment (HPAB-0388-FY-F(E)) | FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY55 | AbPlus™ Anti-CD74 Magnetic Beads (4ALN2) | IP, Protein Purification | |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY71 | CytoStream™ Mouse Anti-CD74 Recombinant Antibody (clone LN2) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY83 | Human Anti-CD74 (clone LL1) scFv-Fc Chimera | FC, ELISA, Cyt | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC330 | Recombinant Anti-CD74 Vesicular Antibody, EV Displayed (VS-0425-YC330) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1217 | Anti-CD74 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1218 | Anti-Mouse CD74 Immunohistochemistry Kit | IHC |
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