Rabbit Anti-CD8A Recombinant Antibody (clone 2F2) (CAT#: ZG-0598J)

Figure 1 Rabbit Anti-CD8A Antibody (ZG-0598J) in WB

Western Blot
Positive WB detected in: MCF-7 whole cell lysate, THP-1 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate
All lanes: CD8A antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 26, 22, 31 kDa
Observed band size: 31 kDa

Figure 2 Rabbit Anti-CD8A Antibody (ZG-0598J) in IHC

IHC image of ZG-0598J diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Figure 3 Rabbit Anti-CD8A Antibody (ZG-0598J) in IHC

IHC image of ZG-0598J diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Figure 4 Rabbit Anti-CD8A Antibody (ZG-0598J) in IP

Immunoprecipitating CD8A in HepG2 whole cell lysate
Lane 1: Rabbit control IgG instead of ZG-0598J in HepG2 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2:ZG-0598J(3µg)+ HepG2 whole cell lysate(500µg)
Lane 3: HepG2 whole cell lysate (10µg)