Rat Anti-Cd8a Recombinant Antibody (clone YTS105.18); scFv Fragment (CAT#: HPAB-S0183-YC-S(P))
Unlike YTS156.7, the YTS105.18 epitope is exposed in the pMHCI/CD8αβ complex. The interacting elements between mouse CD8α and the rat antibody YTS105.18 have been identified in the CD8αα/YTS105.18 Fab co-crystal structure. The two potential models for the pMHCI/CD8αβ complex, in which CD8α occupies an α1 or α2 position, respectively, indicate that inhibition of pMHCI/CD8αβ interaction by mAb YTS105.18 would occur only if CD8αβ binds pMHCI with CD8α in the α1 position, whereas YTS105.18 Fab would not block pMHCI/CD8αβ interactions in which CD8α adopts an α2 position.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SH-SY5Y
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37756/1373_A3_1_selected.jpg
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37756/1373_A3_1_selected.jpg
Figure 2 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37756/1492_D3_2_blue_red_green.jpg
(Immunofluorescent staining of human cell line RH-30 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37756/1492_D3_2_blue_red_green.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37756/1377_A3_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37756/1377_A3_1_blue_red_green.jpg
Figure 4 Lymph node
(Germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12/97_A_7_8.jpg
(Germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12/97_A_7_8.jpg
Figure 5 RNA cell line category: Cell line enhanced (JURKAT, MOLT-4, RPMI-8226)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000153563-CD8A
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000153563-CD8A
Specifications
- Host Species
- Rat
- Type
- Rat scFv
- Specificity
- Mouse CD8A
- Species Reactivity
- Mouse
- Clone
- YTS105.18
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone YTS105.18"
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Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-369-VHH | Recombinant Anti-Mouse Cd8a VHH Single Domain Antibody | IHC, ICC, IP, ChiP, Neut | Llama VHH |
| NABG-041 | Recombinant Anti-Human CD8A VHH Single Domain Antibody | IHC, FC, CA, FuncS | Llama VHH |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-184-S(P) | Recombinant Anti-human CD8A Antibody scFv Fragment | IP, IF, Biosensors, FuncS | scFv |
| MHH-184-S(P) | Recombinant Human Anti-human CD8A Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| FAMAB-0009-CN-S(P) | Mouse Anti-CD8A Recombinant Antibody (clone CT8); scFv Fragment | FC | Mouse scFv |
| FAMAB-0010-CN-S(P) | Mouse Anti-CD8A Recombinant Antibody (clone EP72); scFv Fragment | FC | Mouse scFv |
| FAMAB-0011-CN-S(P) | Mouse Anti-CD8A Recombinant Antibody (clone 3-298); scFv Fragment | FC | Mouse scFv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABZ-144 | Rat Anti-CD8A Recombinant Antibody (clone YTS 105.18) | Neut | Rat IgG |
| HPAB-0188-CN | Mouse Anti-CD8A Recombinant Antibody (HPAB-0188-CN) | FC | Mouse IgG |
| HPAB-0189-CN | Mouse Anti-CD8A Recombinant Antibody (HPAB-0189-CN) | FC | Mouse IgG |
| HPAB-0037-YJ | Human Anti-CD8A Recombinant Antibody (HPAB-0037-YJ) | IA | Humanized IgG |
| FAMAB-1088CQ | Human Anti-CD8A Recombinant Antibody (clone YTC 141.1HL) | FC, IHC | Human IgG1 |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-144 | Rat Anti-CD8A Recombinant Antibody (clone YTS 105.18); scFv Fragment | Neut | Rat scFv |
| HPAB-0188-CN-F(E) | Mouse Anti-CD8A Recombinant Antibody; Fab Fragment (HPAB-0188-CN-F(E)) | FC | Mouse Fab |
| HPAB-0189-CN-F(E) | Mouse Anti-CD8A Recombinant Antibody; Fab Fragment (HPAB-0189-CN-F(E)) | FC | Mouse Fab |
| FAMAB-0009-CN-F(E) | Mouse Anti-CD8A Recombinant Antibody (clone CT8); Fab Fragment | FC | Mouse Fab |
| FAMAB-0010-CN-F(E) | Mouse Anti-CD8A Recombinant Antibody (clone EP72); Fab Fragment | FC | Mouse Fab |
Mouse Antibody
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-428CQ | Mouse Anti-CD8A Recombinant Antibody (clone UCHT4) | FC, IHC-Fr, IHC-P, IP, Separation, Block | Mouse IgG2a, κ |
| NEUT-431CQ | Rat Anti-CD8A Recombinant Antibody (clone 53-6.7) | ICC, IF, FC, IHC-Fr, IP, FuncS, Block | Rat IgG2a, κ |
| NEUT-432CQ | Rat Anti-CD8A Recombinant Antibody (clone MAB0811) | Block, Depletion | Rat IgG2a |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-429CQ | Mouse Anti-CD8A Recombinant Antibody (clone CT-8) | Neut, FC, IHC, IHC-Fr, IHC-P, IP, ICC | Mouse IgG1, κ |
| NEUT-430CQ | Mouse Anti-CD8A Recombinant Antibody (clone LT8) | WB, Neut | Mouse IgG1 |
| NEUT-433CQ | Mouse Anti-CD8A Recombinant Antibody (clone 76-2-11) | Neut, FC, IHC-Fr, IHC-P, IP | Mouse IgG2a, κ |
| NEUT-434CQ | Mouse Anti-Cd8a Recombinant Antibody (clone OX-8) | WB, Neut, FC, IHC, IHC-Fr, IHC-P, IP, CyTOF® | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0582 | Hi-Affi™ Rabbit Anti-CD8A Recombinant Antibody (clone DS582AB) | IHC | Rabbit IgG |
| MOR-0589 | Hi-Affi™ Rabbit Anti-CD8A Recombinant Antibody (clone DS589AB) | IHC-P | Rabbit IgG |
| MOR-0590 | Hi-Affi™ Rabbit Anti-CD8A Recombinant Antibody (clone DS590AB), PE | FC | Rabbit IgG |
| MOR-0057-FY | Rabbit Anti-CD8A Recombinant Antibody (clone AFY0028) | ICC, IF, IHC-P, WB | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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