What is the specificity of the clone YTS105.18?

The YTS105.18 clone specifically recognizes the Mouse CD8A antigen. Interestingly, its epitope is exposed in the pMHCI/CD8αβ complex. Structural studies indicate that this antibody can inhibit the pMHCI/CD8αβ interaction only if CD8α occupies the α1 position in the complex.

How should I store this antibody for long-term use?

For long-term storage, we recommend aliquoting the antibody and storing it at -20°C. You should avoid repeated freeze/thaw cycles to maintain the antibody's stability and activity. For short-term use (1-2 weeks), it can be stored at +4°C.

Rat Anti-Cd8a Recombinant Antibody (clone YTS105.18)

CAT#: HPAB-S0183-YC

Unlike YTS156.7, the YTS105.18 epitope is exposed in the pMHCI/CD8αβ complex. The interacting elements between mouse CD8α and the rat antibody YTS105.18 have been identified in the CD8αα/YTS105.18 Fab co-crystal structure. The two potential models for the pMHCI/CD8αβ complex, in which CD8α occupies an α1 or α2 position, respectively, indicate that inhibition of pMHCI/CD8αβ interaction by mAb YTS105.18 would occur only if CD8αβ binds pMHCI with CD8α in the α1 position, whereas YTS105.18 Fab would not block pMHCI/CD8αβ interactions in which CD8α adopts an α2 position.

Published Data Gene Expression

Figure 1 YTS156.7.7, but not YTS105.18, blocks MHC class I and CD8 interaction and subsequent T cell activation.

(a) Ca²⁺ mobilization in OT-I RAG^- splenocytes treated with H-2K^b tetramers loaded with the OVA peptide after pre-staining with mAbs 53.6.7 (red line), YTS105.18 (purple line) or YTS156.7.7 (green line). The tetramers were added at 1 min (arrow) and the sample re-analyzed for a further 4 min. The H-2K^b/OVA tetramer trace is taken from a 5 min time course and overlaid here as a representation of a responding population. Response to the H-2K^b/OVA tetramers in the absence of CD8 antibodies is shown (blue line), as well as response to H-2K^b/SIYR tetramers (black line) as a negative control. All traces indicate the median of the responding population. (b) Equivalent plot to show relative Ca²⁺ mobilization in OT-I RAG^- splenocytes treated with H-2K^b/OVA tetramers after pre-staining now with only the Fabs of mAbs 53.6.7 (red line), YTS105.18 (purple line) or YTS156.7.7 (green line). The baselines for all traces are normalized to the response to H-2K^b/OVA tetramers in the absence of any Fabs (blue line).

Shore, D. A., Issafras, H., Landais, E., Teyton, L., & Wilson, I. A. (2008). The crystal structure of CD8 in complex with YTS156. 7.7 Fab and interaction with other CD8 antibodies define the binding mode of CD8 αβ to MHC class I. Journal of molecular biology,384(5), 1190-1202.

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Subcellular Location and Protein Expression Figure 1 IF staining of human cell line SH-SY5Y

Immunofluorescent staining of human cell line SH-SY5Y shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37756/1373_A3_1_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line RH-30

Immunofluorescent staining of human cell line RH-30 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37756/1492_D3_2_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37756/1377_A3_1_blue_red_green.jpg

Normal Tissue Figure 4 Lymph node

Germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12/97_A_7_8.jpg

RNA Expression Figure 5 RNA cell line category: Cell line enhanced (JURKAT, MOLT-4, RPMI-8226)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000153563-CD8A

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Specifications

Host Species
Rat

Type
Rat IgG

Specificity
Mouse CD8A

Species Reactivity
Mouse

Clone
YTS105.18

Applications
ELISA

Product Property

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Buffer
PBS

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
Ly-2; Ly-B; Ly-35; Lyt-2; BB154331

Gene ID
12525

UniProt ID
P01731

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Breast cancer biomarkers at key points during disease progression

Precise Epitope Mapping

We chose this rat antibody because the YTS105.18 epitope is known to be exposed in the pMHCI/CD8αβ complex. It performed beautifully in our ELISA setups, allowing us to study these specific immune interactions without interference. The recombinant production ensures we get the same performance with every lot.

Effective For Mouse Cd8a

This product was highly effective for detecting Mouse CD8A in our samples. The purity was confirmed by SDS-PAGE and HPLC, which gave us confidence in our quantitative results. It is a robust tool for investigating cytotoxic T lymphocyte surface glycoproteins in a research setting.

Q&As

What is the specificity of the clone YTS105.18?

A: The YTS105.18 clone specifically recognizes the Mouse CD8A antigen. Interestingly, its epitope is exposed in the pMHCI/CD8αβ complex. Structural studies indicate that this antibody can inhibit the pMHCI/CD8αβ interaction only if CD8α occupies the α1 position in the complex.
How should I store this antibody for long-term use?

A: For long-term storage, we recommend aliquoting the antibody and storing it at -20°C. You should avoid repeated freeze/thaw cycles to maintain the antibody's stability and activity. For short-term use (1-2 weeks), it can be stored at +4°C.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-S0183-YC.pdf

MSDS

MSDS - HPAB-S0183-YC.pdf

COA

Certificate of Analysis Lookup

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