Rabbit Anti-CDK4 Recombinant Antibody (clone 8F2) (CAT#: ZG-0612J)

This product is a rabbit antibody that recognizes CDK4.

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Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

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We require custom production
  • Tested Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
WB

Figure 1 Rabbit Anti-CDK4 Antibody (ZG-0612J) in WB

Figure 1 Rabbit Anti-CDK4 Antibody (ZG-0612J) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate
All lanes: CDK4 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34, 21 kDa
Observed band size: 34 kDa

IP

Figure 2 Rabbit Anti-CDK4 Antibody (ZG-0612J) in IP

Figure 2 Rabbit Anti-CDK4 Antibody (ZG-0612J) in IP

Immunoprecipitating CDK4 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of ZG-0612J in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/1500)
Lane 2:ZG-0612J(2µg)+ Hela whole cell lysate(500µg)
Lane 3: Hela whole cell lysate (10µg)


Specifications

  • Immunogen
  • A synthesized peptide derived from human CDK4
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human CDK4
  • Species Reactivity
  • Human
  • Clone
  • 8F2
  • Applications
  • ELISA, WB, IP
  • Conjugate
  • Uconjugated
  • Related Disease
  • Cancer

Product Property

  • Purification
  • Affinity purified
  • Format
  • Liquid
  • Concentration
  • See the COA
  • Buffer
  • PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunoprecipitation.
    WB:1:500-1:5000, IP:1:200-1:1000

Target

  • Alternative Names
  • CMM3; PSK-J3; cyclin-dependent kinase 4; cell division protein kinase 4

Related Resources

  • Related Diseases
  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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