Human Anti-CDK4 Recombinant Antibody (clone AK2) (CAT#: VS-0423-CJ3)

• Host Species
• Human

• Type
• Human IgG

• Specificity
• Human CDK4

• Species Reactivity
• Human

• Clone
• AK2

• Applications
• ELISA, IF

• Conjugate
• Unconjugated

• Related Disease
• Tumors

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• PBS

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been reported for use in ELISA, Immunofluorescence.

• Alternative Names
• CMM3; PSK-J3

• Gene ID
• 1019

• UniProt ID
• P11802

• Sequence Similarities
• Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

• Cellular Localization
• Cytoplasm, Membrane, Nucleus

• Post Translation Modifications
• Phosphorylation at Thr-172 is required for enzymatic activity. Phosphorylated, in vitro, at this site by CCNH-CDK7, but, in vivo, appears to be phosphorylated by a proline-directed kinase. In the cyclin D-CDK4-CDKN1B complex, this phosphorylation and consequent CDK4 enzyme activity, is dependent on the tyrosine phosphorylation state of CDKN1B. Thus, in proliferating cells, CDK4 within the complex is phosphorylated on Thr-172 in the T-loop. In resting cells, phosphorylation on Thr-172 is prevented by the non-tyrosine-phosphorylated form of CDKN1B.

• Protein Refseq
• NP_000066.1

• Function
• Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Isotype Control

• MRO-A1587-YJ
• Human IgG Isotype control, Polyclonal

Secondary Antibody

• 2ND-024CQ
• Goat Anti-Human IgG (H&L) Antibody

• 2ND-025CQ
• Rabbit Anti-Human IgG (H&L) Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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